A5072477237- Cardiac Valve Diseases and Treatments
- Mollusks and Parasites Studies
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Bone Metabolism and Diseases
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Infective Endocarditis Diagnosis and Management
- Advanced Proteomics Techniques and Applications
- Extracellular vesicles in disease
- Insect and Arachnid Ecology and Behavior
- Marine Biology and Environmental Chemistry
- Bone and Dental Protein Studies
- Metabolomics and Mass Spectrometry Studies
- Periodontal Regeneration and Treatments
- Parasite Biology and Host Interactions
- Marine Biology and Ecology Research
- Cancer, Hypoxia, and Metabolism
- Orthopedic Infections and Treatments
- Microbial Community Ecology and Physiology
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Genetic diversity and population structure
- Aquatic Invertebrate Ecology and Behavior
- Polar Research and Ecology
Institute of Cytology
2019-2025
Weizmann Institute of Science
2023-2025
St Petersburg University
2015-2022
Federal Almazov North-West Medical Research Centre
2022
For decades, antigen presentation on major histocompatibility complex class I for T cell-mediated immunity has been considered the primary function of proteasome-derived peptides1,2. However, whether products proteasomal degradation play additional parts in mounting immune responses remains unknown. Antimicrobial peptides serve as a first line defence against invading pathogens before adaptive system responds. Although protective antimicrobial across numerous tissues is well established,...
Dental stem cells are heterogeneous in their properties. Despite common origin from neural crest cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp (DPSC) periodontal ligament (PDLSC) pluripotency neuroepithelial markers transcription, morphological features, osteoblast/odontoblast differentiation proteomic profile during osteogenic differentiation. The data were collected paired...
Calciprotein particles (CPPs) represent an inherent mineral buffering system responsible for the scavenging of excessive Ca2+ and PO43− ions in order to prevent extraskeletal calcification, although contributing development endothelial dysfunction during circulation bloodstream. Here, we performed label-free proteomic profiling identify functional consequences CPP internalisation by cells (ECs) found molecular signatures significant disturbances mitochondrial lysosomal physiology, including...
ABSTRACT The assembly of proteins into functional complexes is critical to life’s processes. While textbooks depict complex as occurring between fully synthesized proteins, we know today that thousands in the human proteome assemble co-translationally during their synthesis. Why this process takes place, however, remains unknown. We show co-translational governed by biophysical and structural characteristics protein complex, involves mutually stabilized, intertwined subunits. Consequently,...
The lack of suitable autologous grafts and the impossibility using synthetic prostheses for small artery reconstruction make it necessary to develop alternative efficient vascular grafts. In this study, we fabricated an electrospun biodegradable poly(ε-caprolactone) (PCL) prosthesis poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) (PHBV/PCL) loaded with iloprost (a prostacyclin analog) as antithrombotic drug cationic amphiphile antibacterial activity. were characterized in...
Major adverse cardiovascular events occurring upon coronary artery bypass graft surgery are typically accompanied by endothelial dysfunction. Total arterial revascularisation, which employs both left and right internal thoracic arteries instead of the saphenous vein to create a bypass, is associated with better mid- long-term outcomes. We suggested that molecular profiles human cells (HCAECs) mammary (HITAECs) coherent in terms transcriptomic proteomic signatures, were then investigated RNA...
Calciprotein particles (CPPs) are indispensable scavengers of excessive Ca2+ and PO43− ions in blood, being internalised recycled by liver spleen macrophages, monocytes, endothelial cells (ECs). Here, we performed a pathway enrichment analysis cellular compartment-specific proteomes primary human coronary artery ECs (HCAEC) internal thoracic (HITAEC) treated with (amorphous) or secondary (crystalline) CPPs (CPP-P CPPs, respectively). Exposure to CPP-P CPP-S induced notable upregulation of:...
Abstract Osteogenic differentiation is crucial in normal bone formation and pathological calcification, such as calcific aortic valve disease (CAVD). Understanding the proteomic transcriptomic landscapes underlying this can unveil potential therapeutic targets for CAVD. In study, we employed RNA sequencing transcriptomics proteomics on a timsTOF Pro platform to explore multiomics profiles of interstitial cells (VICs) osteoblasts during osteogenic differentiation. For proteomics, utilized 3...
Heart valve calcification is an active cellular and molecular process that partly remains unknown. Osteogenic differentiation of interstitial cells (VIC) a central mechanism in calcific aortic disease (CAVD). Studying mechanisms CAVD progression clearly needed. In this study, we compared osteogenic human VIC isolated from healthy donors or patients with by RNA-seq transcriptomics early timepoint (48 h) shotgun proteomics at later (10th day). Bioinformatic analysis revealed genes pathways...
Abstract Mesenchymal stem cells (MSCs) are widely used in therapy, but the differences between MSCs of various origins and their ability to undergo osteogenic differentiation produce extracellular matrix not fully understood. To address this, we conducted a comparative analysis mesenchymal cell primary cultures from 6 human sources, including osteoblast-like adult femur, adipose-derived cells, Wharton’s jelly-derived gingival fibroblasts, dental pulp periodontal ligament cells. We analyzed...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 552:177-193 (2016) - DOI: https://doi.org/10.3354/meps11770 Measuring physiological similarity of closely related littorinid species: a proteomic insight Arina L. Maltseva1,*, Marina A. Varfolomeeva1, Arseniy Lobov1, Natalia Mikhailova1,2, Paul E. Renaud3, Alexey V. Grishankov1, Kseniia Y. Volovik1, Andrei I....
Background The introduction of DNA-based molecular markers made a revolution in biological systematics. However, cases very recent divergence events, the neutral may be too slow, and analysis adaptive part genome is more informative to reconstruct evolutionary history young species. advantage proteomics its ability reflect biochemical machinery life. It help both identify rapidly evolving genes interpret their functions. Methods Here we applied comparative gel-based proteomic several species...
Despite the popularity of mesenchymal stem cells (MSCs), many fundamental aspects their physiology still have not been understood. The information accumulated to date argues that MSCs from different sources vary in differentiation potential and, probably, molecular mechanisms trilineage differentiation. Therefore, this review consists two parts. Firstly, we focus on data inter- and intra-source variation MSCs. We discuss detail MSC at single-cell level direct omics comparison four main...
The culture of osteoblasts (OB) human origin is a useful experimental model in studying bone biology, osteogenic differentiation, functions proteins, oncological processes tissue, testing drugs against desires, and many other fields. purpose the present study to share workflow that has established conditions efficiently isolate grow OB cells obtained from surgically removed bones donors. protocol described here also shows how determine cell phenotype. Here we provide characteristics isolated...
Implementation of small-diameter tissue-engineered vascular grafts (TEVGs) into clinical practice is still delayed due to the frequent complications, including thrombosis, aneurysms, neointimal hyperplasia, calcification, atherosclerosis, and infection. Here, we conjugated a vasodilator/platelet inhibitor, iloprost, an antimicrobial cationic amphiphilic drug, 1,5-bis-(4-tetradecyl-1,4-diazoniabicyclo [2.2.2]octan-1-yl) pentane tetrabromide, luminal surface electrospun poly(ε-caprolactone)...
Despite the great progress in field of bone tissue regeneration, early initiating mechanisms osteogenic differentiation are not well understood. Cells capable transformation vary from mesenchymal stem cells various origins to mural vessels. The pathological calcification thought be similar those formation. Notch signaling has been shown play an important role differentiation, as calcification. Nevertheless, despite its known tissue- and context-specificity, information about different is...
Calcific aortic valve disease (CAVD) is one of the dangerous forms vascular calcification. CAVD leads to calcification and disturbance blood flow. Despite high mortality, there no targeted therapy against or Osteogenic differentiation interstitial cells (VICs) key factors progression inhibition this process seems a fruitful target for potential therapy. By our previous study we assumed that inhibitors Notch pathway might be effective suppress leaflet We tested CB-103 crenigacestat...
Abstract Osteogenic differentiation is crucial in normal bone formation and pathological calcification, such as calcific aortic valve disease (CAVD). Understanding the proteomic transcriptomic landscapes underlying this can unveil potential therapeutic targets for CAVD. In study, we employed timsTOF Pro platform to explore profiles of interstitial cells (VICs) osteoblasts during osteogenic differentiation, utilizing three data acquisition/analysis techniques: Data-Dependent Acquisition...
Abstract Reproductive isolation is the key attribute of biological species and establishment reproductive barriers an essential event for speciation. Among mechanisms isolation, gamete incompatibility due to variability interaction proteins may drive fast divergence even in sympatry. However, number available models study this phenomenon limited. In case internally fertilized invertebrates, sperm competition are restricted a single taxon: insects. Here, we propose group closely related...
North Atlantic Littorina species ( periwinkles) of the subgenus Neritrema are represented by two groups morphologically and phylogenetically closely related species-two sister 'obtusata' group: obtusata (Linnaeus, 1758) L.
Background Whereas the risk factors for structural valve degeneration (SVD) of glutaraldehyde-treated bioprosthetic heart valves (BHVs) are well studied, those responsible failure BHVs fixed with alternative next-generation chemicals remain largely unknown. This study aimed to investigate reasons behind development SVD in ethylene glycol diglycidyl ether-treated BHVs. Methods and Results Ten excised because SVD, 5 calcified aortic (AVs) replaced calcific AV disease were collected their...