A5085147677- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Intracranial Aneurysms: Treatment and Complications
- Cerebrovascular and Carotid Artery Diseases
- 3D Printing in Biomedical Research
- Electron and X-Ray Spectroscopy Techniques
- Human Health and Disease
- Hemostasis and retained surgical items
- Extracellular vesicles in disease
- Infectious Aortic and Vascular Conditions
- Blood properties and coagulation
- Healthcare Systems and Public Health
- Aortic Disease and Treatment Approaches
- Neurological Disorders and Treatments
- Silk-based biomaterials and applications
- Angiogenesis and VEGF in Cancer
- Periodontal Regeneration and Treatments
- Aortic aneurysm repair treatments
- Polymer Surface Interaction Studies
Kemerovo State University
2019-2023
Russian Academy of Sciences
2015
The lack of suitable autologous grafts and the impossibility using synthetic prostheses for small artery reconstruction make it necessary to develop alternative efficient vascular grafts. In this study, we fabricated an electrospun biodegradable poly(ε-caprolactone) (PCL) prosthesis poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) (PHBV/PCL) loaded with iloprost (a prostacyclin analog) as antithrombotic drug cationic amphiphile antibacterial activity. were characterized in...
Tissue-engineered vascular graft for the reconstruction of small arteries is still an unmet clinical need, despite fact that a number promising prototypes have entered preclinical development. Here we test Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)Poly(ε-caprolactone) 4-mm-diameter grafts equipped with endothelial growth factor (VEGF), basic fibroblast (bFGF) and stromal cell-derived 1α (SDF-1α) surface coated heparin iloprost (PHBV/PCL[VEGF-bFGF-SDF]Hep/Ilo, n = 8) in sheep carotid artery...
Tissue-engineered vascular grafts are widely tested as a promising substitute for both arterial bypass and replacement surgery. We previously demonstrated that incorporation of VEGF into electrospun tubular scaffolds from poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) enhances formation an endothelial cell monolayer. However, overdose can induce tumor-like vasculature; thereby, other bioactive factors needed to support VEGF-driven endothelialization successful recruitment...
Implementation of small-diameter tissue-engineered vascular grafts (TEVGs) into clinical practice is still delayed due to the frequent complications, including thrombosis, aneurysms, neointimal hyperplasia, calcification, atherosclerosis, and infection. Here, we conjugated a vasodilator/platelet inhibitor, iloprost, an antimicrobial cationic amphiphilic drug, 1,5-bis-(4-tetradecyl-1,4-diazoniabicyclo [2.2.2]octan-1-yl) pentane tetrabromide, luminal surface electrospun poly(ε-caprolactone)...
Fibrin is widely used in vascular tissue engineering. Typically, fibrin polymerization initiated by adding exogenous thrombin. In this study, we proposed a protocol for the preparation of completely autologous without use endogenous thrombin and compared properties prepared matrix with that obtained traditional method. Fibrinogen was ethanol precipitation followed either calcium chloride (ExThr), or only (EnThr). We examined structure, mechanical properties, thrombogenicity, degradation rate...
Modification by Arg-Gly-Asp (RGD) peptides is a promising approach to improve the biocompatibility of biodegradable vascular patches for arteriotomy. In this study, we evaluated performance electrospun using blend polycaprolactone (PCL) and polyhydroxybutyrate/valerate (PHBV) additionally modified with RGDK, AhRGD, c[RGDFK] 1,6-hexamethylenediamine or 4,7,10-trioxa-1,13-tridecanediamine (TTDDA) linkers. We examined mechanical properties hemocompatibility resulting before implanting them in...
Objective : to create a cell-populated small-diameter vascular graft (SDVG) using autologous endothelial cells and extracellular matrix proteins, evaluate the efficiency of cell monolayer formation during shear stress preconditioning in SDVG. Materials methods . PHBV/PCL tubular scaffolds grafts were made by electrospinning from mixture polyhydroxybutyrate-valerate (PHBV) copolymer polycaprolactone (PCL) modified with fibrin. To populate graft, an culture was isolated blood patients coronary...
The purpose . Мodification of scanning electron microscopy method in backscattered electrons to research biological samples with massive metal or mineral inclusions. Materials and methods In the article alternative without cutting sections were supplied. Samples for researching (plates from NiTi surrounded by tissues after subcutal implantation, explanted stents, epoxy covered xenoaortic bioprosthetic heart valves , biodegradable vascular grafts rats abdominal aorta) embedded risin,...
We prepared polycaprolactone (PCL) and polyhydroxybutyrate/valerate (PHBV)/polycaprolactone 2 mm vascular grafts using electrospinning. The structure of the graft surface along with physico-mechanical viscoelastic properties were investigated by scanning electron microscopy, universal testing machine (n=20), arterial pulsatile flow circuit (n=12), respectively. also assessed hemocompatibility thromboresistance inner (n=12). Cell adhesion viability EA.hy 926, an endothelial cell line that was...
An important area of tissue engineering is the development small-dimeter vascular grafts. In our study we assessed whether an electrospun polymer scaffold beneficial for developing a tissue-engineered graft. The polymeric grafts were made from blend polyhydroxybutyrate/valerate and polycaprolactone (PHBV/PCL) added type I collagen. Additionally, treated with fibronectin. Cells isolated peripheral blood patients coronary artery disease (CAD) then seeded on luminal graft surface. Then, cells...
Carotid endarterectomy using vascular patches is associated with a large number of complications, thereby necessitating the development novel biocompatible materials suitable for reconstruction wall. A promising approach to overcome this problem use combination biodegradable polymer and bioactive molecules that stimulate regeneration tissue. In study, porous were made from polycaprolactone (PCL) incorporated endothelial growth factor (VEGF) emulsion electrospinning. Their morphology,...
Background. Commercially available synthetic and animal-derived vascular patches used in patch angioplasty during carotid endarterectomy have several disadvantages, such as postoperative thrombosis or occlusion restenosis. This problem may be resolved by the development of biologically active materials that are biodegradable can stimulate tissue regeneration.Aim. To evaluate properties efficacy a based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) poly(ε-caprolactone) (PCL) into...
Purpose. To evaluate the local angiogenesis stimulation by polycaprolactone (PCL) scaffolds with VEGF in vivo. Materials and methods. PCL were fabricated using electrospinning method. For encapsulation of into scaffold a two phase was used. The structure examined scanning electron microscopy. Growth factor release dynamic assessed ELISA. evaluation biological properties, PCL+VEGF implanted Wistar rat abdominal wall (n=60) for 2, 3 4 monthsT. he explanted samples histological...
In the course of research work we examined 30 patients with hypertensive disease and 50 somatically healthy persons Astrakhan region as a control group. The study functional state vascular endothelium was conducted by method laser doppler flowmetry. It was revealed, that all demonstrated development of endothelial dysfunction different degrees severity (dysfunction medium degree — in 69%, significant dysfunction 31% patients). feature reactivity microvascular is predominance...
The aim of the investigation was to assess effectiveness using endothelial colony-forming cells (ECFCs) for populating polymer vascular grafts under conditions a pulsatile bioreactor.Materials and Methods.Grafts were fabricated by electrospinning from mixture poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(ε-caprolactone), type I collagen.To carry out experiments, commercial culture primary human coronary artery (HCAEC), umbilical vein (HUVEC), ECFCs obtained peripheral blood patients...