A5043106657- Cancer Cells and Metastasis
- Ferroptosis and cancer prognosis
- TGF-β signaling in diseases
- Adenosine and Purinergic Signaling
- Neonatal Respiratory Health Research
- Cancer Immunotherapy and Biomarkers
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Tissue Engineering and Regenerative Medicine
- Radiomics and Machine Learning in Medical Imaging
- Respiratory Support and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Cell death mechanisms and regulation
- Spaceflight effects on biology
- Nanoplatforms for cancer theranostics
- Pediatric health and respiratory diseases
- Bone Tissue Engineering Materials
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- Erythrocyte Function and Pathophysiology
- Cellular Mechanics and Interactions
- Bladder and Urothelial Cancer Treatments
- Cardiac Fibrosis and Remodeling
Vanderbilt University Medical Center
2020-2025
Vanderbilt University
2019-2024
Nashville Oncology Associates
2023
<title>Abstract</title> Background Antibody-based therapies (such as anti-EGFR and anti-PD1/L1 agents) have altered the landscape of cancer treatment to improve patient outcomes in formerly unresponsive tumor types. However, this robust response is not ubiquitous for all patients or subtypes. Head neck squamous cell carcinoma continues reduced many populations regardless target expression (e.g. EGFR PDL1). The role microenvironmental proteins, such fibroblast activation protein (FAP), may...
Abstract TGFβ plays a crucial role in the tumor microenvironment by regulating cell–cell and cell–stroma interactions. We previously demonstrated that signaling on myeloid cells regulates expression of CD73, key enzyme for production adenosine, protumorigenic metabolite implicated regulation cell behaviors, immune response, angiogenesis. Here, using an MMTV-PyMT mouse mammary model, we discovered deletion (PyMT/TGFβRIILysM) affects extracellular matrix (ECM) formation tissue, specifically...
Soldiers deployed to Iraq and Afghanistan have a higher prevalence of respiratory symptoms than nondeployed military personnel some been shown constellation findings on lung biopsy termed post-deployment syndrome (PDRS). Since many the subjects in this cohort reported exposure sulfur dioxide (SO
Although activation of adaptive immunity is a common pathological feature chronic obstructive pulmonary disease (COPD), particularly during later stages the disease, underlying mechanisms are poorly understood. In small airways COPD patients, we found that localized disruption secretory immunoglobulin A (SIgA)-containing mucosal immunobarrier correlated with lymphocyte accumulation in airway walls and development tertiary lymphoid structures (TLS) around airways. SIgA-deficient mice,...
Lung adenocarcinoma (ADC) is a heterogeneous group of tumors associated with different survival rates, even when detected at an early stage. Here, we aim to investigate whether CyTOF identifies cellular and molecular predictors tumor behavior. We developed validated panel 34 antibodies in four ADC cell lines PBMC. tested our set 10 ADCs, classified into long- (LPS) (n = 4) short-predicted (SPS) 6) based on radiomics features. identified subpopulations epithelial cancer cells (ECC) their...
Cancer associated fibroblasts (CAF) play a key role in cancer progression and metastasis. Diminished TGFβ response on CAF correlates with poor outcome recurrence patients. Mechanisms behind lost signaling are poorly understood, but, utilizing MMTV-PyMT mouse model, we have previously demonstrated that tumor microenvironment myeloid cells, producing adenosine, contribute to downregulated CAFs. In the current work, performed serial vitro studies investigate of adenosine/TGFβ axis mammary...
Lung cancer is the deadliest in United States and worldwide, lung adenocarcinoma (LUAD) most prevalent histologic subtype States. LUAD exhibits a wide range of aggressiveness risk recurrence, but biological underpinnings this behavior are poorly understood. Past studies have focused on characteristics tumor itself, ability immune response to contain growth represents an alternative or complementary hypothesis. Emerging technologies enable us investigate spatial distribution specific cell...
Growth arrest-specific 6 (Gas6) has been implicated in carcinogenesis through activation of its receptors, particularly MerTK. To investigate whether Gas6 plays a role resistance to NF-κB inhibitors, which have not proven be effective agents for lung cancer therapy, we studied models induced by urethane injection or expression mutant Kras (KrasG12D). We found that is primarily produced macrophages during tumorigenesis and negatively regulated NF-κB. Since vitamin K dependent protein, used...
Lung adenocarcinoma (LUAD) is the predominant type of lung cancer in U.S. and exhibits a broad variety behaviors ranging from indolent to aggressive. Identification biological determinants LUAD behavior at early stages can improve existing diagnostic treatment strategies. Extracellular matrix (ECM) remodeling cancer-associated fibroblasts play crucial role regulation aggressiveness there growing need investigate their determination stages. We analyzed tissue samples isolated patients with...
Modern technologies designed for tissue structure visualization like brightfield microscopy, fluorescent mass cytometry imaging (MCI) and spectrometry (MSI) provide large amounts of quantitative spatial information about cells structures vessels, bronchioles etc. Many published reports have demonstrated that the structural features extracellular matrix (ECM) their interactions strongly predict disease development progression. Computational image analysis methods in combination with machine...
Abstract TGF-beta plays a crucial role in tumor microenvironment by regulating cell-cell and cell-stroma interactions. We previously demonstrated that signaling on myeloid cells regulates expression of CD73, key enzyme for production adenosine, pro-tumorigenic metabolite implicated regulation cell behaviors, immune response angiogenesis. Using MMTV-PyMT mouse mammary model, we discovered deletion (PyMT/TGFbRII-LysM) affects ECM formation tissue, specifically increases collagen decreases...
<div>Abstract<p>TGFβ plays a crucial role in the tumor microenvironment by regulating cell–cell and cell–stroma interactions. We previously demonstrated that TGFβ signaling on myeloid cells regulates expression of CD73, key enzyme for production adenosine, protumorigenic metabolite implicated regulation cell behaviors, immune response, angiogenesis. Here, using an MMTV-PyMT mouse mammary model, we discovered deletion (PyMT/TGFβRII<sup>LysM</sup>) affects extracellular...
<p>Analysis all available cancer types in TCGA data sets. Correlation analysis of METABRIC between stages or aggressiveness vs. ECM proteins expression.</p>
<p>Analysis all available cancer types in TCGA data sets. Correlation analysis of METABRIC between stages or aggressiveness vs. ECM proteins expression.</p>
Soldiers deployed to Iraq and Afghanistan have a higher prevalence of respiratory symptoms than non-deployed military personnel some been shown constellation findings on lung biopsy termed post-deployment syndrome (PDRS). Since many the deployers in this cohort reported exposure sulfur dioxide (SO
<div>Abstract<p>TGFβ plays a crucial role in the tumor microenvironment by regulating cell–cell and cell–stroma interactions. We previously demonstrated that TGFβ signaling on myeloid cells regulates expression of CD73, key enzyme for production adenosine, protumorigenic metabolite implicated regulation cell behaviors, immune response, angiogenesis. Here, using an MMTV-PyMT mouse mammary model, we discovered deletion (PyMT/TGFβRII<sup>LysM</sup>) affects extracellular...
Purpose. To evaluate the local angiogenesis stimulation by polycaprolactone (PCL) scaffolds with VEGF in vivo. Materials and methods. PCL were fabricated using electrospinning method. For encapsulation of into scaffold a two phase was used. The structure examined scanning electron microscopy. Growth factor release dynamic assessed ELISA. evaluation biological properties, PCL+VEGF implanted Wistar rat abdominal wall (n=60) for 2, 3 4 monthsT. he explanted samples histological...
Abstract Tumor microenvironment (TME) represents a dynamic niche that regulates cancer cell behavior contributing directly to disease outcome. Systemic approach analysis of TME should uncover its complexity and facilitate discovery mechanisms orchestrating tumor development metastasis. Multiplexed fluorescent tissue stain followed by spatial architecture can provide insights pivotal interactions cellular acellular components TME. Extracellular matrix (ECM), one constitutive TME, is...
Abstract Lung adenocarcinoma (ADC) is a heterogeneous group of tumors associated with dramatically different survival rates, even when detected at an early stage. We hypothesized that single cell proteomic approach would allow the dissection cellular determinants lung ADC behavior. developed mass cytometry panel 34 antibodies and validated their performance in four lines (A459, H23, PC9 H3211) immune cells. tested our set 10 stage ADCs, classified into long- (LPS) (n=4) short-predicted (SPS)...
Abstract Tumor microenvironment (TME) represents an integrated system that affects cancer cell behavior and contributes directly to disease outcome. Systemic approach analysis of TME should uncover its complexity facilitate discovery mechanisms orchestrating tumor development metastasis. Multiplex fluorescence tissue staining followed by spatial architecture can provide insights pivotal interactions cellular acellular components TME. Extracellular matrix (ECM is represented mainly collagen...