A5082473453- Thyroid Cancer Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Head and Neck Anomalies
- Cancer Cells and Metastasis
- Ferroptosis and cancer prognosis
- Cervical Cancer and HPV Research
- Thyroid and Parathyroid Surgery
- Wnt/β-catenin signaling in development and cancer
- Ecology and Conservation Studies
- Salivary Gland Tumors Diagnosis and Treatment
- CAR-T cell therapy research
- Protein Degradation and Inhibitors
- Cancer Immunotherapy and Biomarkers
- 3D Printing in Biomedical Research
- Pancreatic and Hepatic Oncology Research
- Neuroendocrine Tumor Research Advances
- Cancer Genomics and Diagnostics
- Microtubule and mitosis dynamics
- Immune Cell Function and Interaction
- Bioinformatics and Genomic Networks
- Global Cancer Incidence and Screening
- Melanoma and MAPK Pathways
- BRCA gene mutations in cancer
- Lung Cancer Research Studies
- Cancer-related Molecular Pathways
Vanderbilt University Medical Center
2016-2025
Vanderbilt University
2014-2025
Università Cattolica del Sacro Cuore
2018-2024
Leipzig University
2024
Beth Israel Deaconess Medical Center
2023-2024
Massachusetts General Hospital
2018-2024
The University of Texas MD Anderson Cancer Center
2019-2024
Breast Cancer Research Foundation
2024
Vanderbilt-Ingram Cancer Center
2022-2024
Loyola Medicine
2024
The prevalence of thyroid carcinoma is increasing and represents the most common endocrine malignancy, with papillary (PTC) being frequent subtype. genetic alterations identified in PTCs fail to distinguish tumors different clinical behaviors, such as extra-thyroidal extension lymph node metastasis. We hypothesize that immune microenvironment may play a critical role tumor invasion Computational immunogenomic analysis was performed on 568 PTC samples Cancer Genome Atlas using CIBERSORT,...
Importance Definitive diagnosis of a thyroid nodule in child is obtained through diagnostic surgery. This problematic because pediatric surgery associated with higher rates complications. In adults, preoperative molecular testing improves the management nodules, but this has not been validated children. Objective To determine whether landscape nodules amenable to detection by multigene genomic classifier (GC) test (ThyroSeq v3; Sonic Healthcare USA). Design, Setting, and Participants was...
Clear cell renal carcinoma (ccRCC) is characterized by dysregulated hypoxia signaling and a tumor microenvironment (TME) highly enriched in myeloid lymphoid cells. Loss of the von Hippel Lindau (VHL) gene critical early event ccRCC pathogenesis promotes stabilization HIF. Whether VHL loss cancer cells affects immune TME remains unclear. Using Vhl WT Vhl-KO vivo murine kidney Renca models, we found that tumors were more infiltrated Tumor-associated macrophages (TAMs) from Vhl-deficient...
The pathogenic nature of cancer is attributed, at least in part, to the ability tumors cells induce systemic and local mechanisms immune tolerance. However, we previously reported that tumor-free survival up 100% tolerized HER-2/neu transgenic mice can be achieved by administration neu-specific mAb concurrently with a HER-2/neu-expressing, GM-CSF-secreting whole cell vaccine. In this report, show one mechanism improved antitumor activity induced combination these 2 neu-targeted interventions...
Patients with MYC/BCL2 double-hit lymphoma (DHL) are known to have an aggressive clinical course and respond poorly various therapies including intensive chemotherapy stem cell transplant. Less is about high-grade B-cell MYC rearrangement without concomitant BCL2 BCL6 rearrangement, designated here as single-hit (SHL). In this study, we assessed 61 cases of SHL compared them 83 DHL, all confirmed by MYC, BCL2, fluorescence in situ hybridization studies. Although many clinicopathologic...
Abstract The relative abundance of Wnt receptors plays a crucial role in controlling signaling tissue homeostasis and human disease. While the ubiquitin ligases that ubiquitylate are well-characterized, deubiquitylase reverses these reactions remains unclear. Herein, we identify USP46, UAF1, WDR20 (USP46 complex) as positive regulators cultured cells. We find USP46 complex is similarly required for Xenopus zebrafish embryos. demonstrate promotes association between cell surface coreceptor,...
The American Thyroid Association guidelines task force currently recommends definitive thyroidectomy or lobectomy after an indeterminate thyroid biopsy in children. This recommendation is based on evidence of a greater incidence and higher risk malignancy compared with adults earlier pediatric studies. Such management may lead to overtreatment unnecessary surgery for many children the United States.The objective current study was re-evaluate nodules assess overall percentages rates biopsies...
Abstract Although metastasis is the most common cause of cancer deaths, metastasis-intrinsic dependencies remain largely uncharacterized. We previously reported that metastatic pancreatic cancers were dependent on glucose-metabolizing enzyme phosphogluconate dehydrogenase (PGD). Surprisingly, PGD catalysis was constitutively elevated without activating mutations, suggesting a non-genetic basis for enhanced activity. Here we report metabolic adaptation stably activates to reprogram chromatin....
The Wnt–β-catenin signal transduction pathway is essential for embryonic development and adult tissue homeostasis. Wnt signaling converts TCF from a transcriptional repressor to an activator in process facilitated by the E3 ligase XIAP. XIAP-mediated monoubiquitylation of corepressor Groucho (also known as TLE) decreases its affinity TCF, thereby allowing coactivator β-catenin displace it on TCF. Through genome-scale screen cultured Drosophila melanogaster cells, we identified deubiquitylase...
Genomic and transcriptomic analysis has furthered our understanding of many tumors. Yet, thyroid cancer management is largely guided by staging histology, with few molecular prognostic treatment biomarkers. Here, we utilize a large cohort 251 patients 312 samples from two tertiary medical centers perform DNA/RNA sequencing, spatial transcriptomics, multiplex immunofluorescence to identify biomarkers aggressive malignancy. We high-risk mutations discover unique signature disease, the...
Thyroid cancer progression from curable well-differentiated thyroid carcinoma to highly lethal anaplastic is distinguished by tumor cell de-differentiation and recruitment of a robust stromal infiltrate. Combining an integrated single-cell sequencing atlas with spatial transcriptomics bulk RNA-sequencing, we define subpopulations tumor-stromal cross-talk occurring across the histologic mutational spectrum cancer. We identify distinct inflammatory myofibroblastic cancer-associated fibroblast...
Abstract Tenascin-C (TNC) is a secreted extracellular matrix protein that highly expressed during embryonic development and re-expressed wound healing, inflammation, neoplasia. Studies in developmental models suggest TNC may regulate the Wnt signaling pathway. Our lab has shown high levels of expression anaplastic thyroid cancer (ATC), lethal with an abysmal ∼3-5 month median survival. Here, we investigated role facilitating ligand-dependent cancer. We utilized bulk RNA-sequencing from three...
<title>Abstract</title> Background Antibody-based therapies (such as anti-EGFR and anti-PD1/L1 agents) have altered the landscape of cancer treatment to improve patient outcomes in formerly unresponsive tumor types. However, this robust response is not ubiquitous for all patients or subtypes. Head neck squamous cell carcinoma continues reduced many populations regardless target expression (e.g. EGFR PDL1). The role microenvironmental proteins, such fibroblast activation protein (FAP), may...
Thyroid hormones (THs: T 3 and 4 ) are key regulators of metabolic rate nutrient metabolism. They controlled centrally peripherally in a coordinated manner to elegantly match -mediated energy expenditure (EE) availability. Hypothyroidism reduces EE has long been blamed for obesity; however, emerging evidence suggests that, instead, obesity may drive thyroid dysfunction. Thus, we used mouse model diet-induced determine its direct effects on histopathology function, deiodinase activity,...
Abstract Thyroid cancers exist on a continuum of differentiation. Our group recently defined dynamic changes in the tumor microenvironment co-occurring with histotypes and de-differentiation. Here, we integrate 423, 733 cells from seven distinct single-cell RNA-sequencing cohorts to define unique thyroid cancer- specific fibroblast subtypes occurring across histologic mutational spectrum cancer. We identify inflammatory, perivascular-like, myofibroblastic cancer-associated (myCAF)...
Background Cancer vaccines are designed to activate and enhance cancer-antigen-targeted T cells that suppressed through multiple mechanisms of immune tolerance in cancer-bearing hosts. regulatory cell (Treg) suppression tumor-specific is one barrier effective immunization. A second mechanism the deletion high avidity cells, which leaves a less low tumor specific repertoire available for activation by vaccines. Treg depleting agents including dose cyclophosphamide (Cy) antibodies deplete...
Abstract Background It is not known how much information clients retrieve from discharge instructions. Objective To investigate client's understanding of instructions and influencing factors. Animals Dogs cats being hospitalized for neurological diseases. Methods Clients were presented questionnaires regarding their pet's disease, diagnostics, treatments, prognosis at time 2 weeks later. The same questions answered by discharging veterinarians discharge. additional the subjective feelings...
Abstract Background Anaplastic thyroid carcinoma (ATC) is a highly aggressive malignancy that has consistently shown Wnt/β-catenin (canonical) signaling activation in various study populations. There are currently no targetable treatments for BRAF -wildtype ATC and lack of effective treatment V600E ATC. Our aim to identify whether Wnt inhibitors could be potential therapeutic agents patients with limited options. Methods In this Institutional Review Board-approved study, we utilize cohort 32...
The characteristic features of Papanicolaou (Pap) tests collected from female-to-male (FTM) transgender patients on androgen therapy have not been well defined in the literature. FTM require cervical cancer screening with same recommended frequency as cis-gender females. Dysplasia remains challenging to differentiate atrophy. Without pertinent history, atrophic findings younger can be misinterpreted high-grade dysplasia.A review all Pap receiving (2010-2017) was performed. Bethesda...
Abstract Tumor-associated macrophages (TAM) play an important role in supporting tumor growth and suppressing antitumor immune responses, TAM infiltration has been associated with poor patient prognosis various cancers. TAMs can be classified as pro-inflammatory, M1-like, or anti-inflammatory, M2-like. While multiple factors within the microenvironment affect recruitment, polarization, functions of TAMs, accumulating evidence suggests that Wnt signaling represents important, targetable...