A5029633247- Blood properties and coagulation
- Parathyroid Disorders and Treatments
- Biomarkers in Disease Mechanisms
- Cardiac Valve Diseases and Treatments
- Advanced Proteomics Techniques and Applications
- Angiogenesis and VEGF in Cancer
- Cerebrovascular and Carotid Artery Diseases
- Cardiovascular Disease and Adiposity
- Electrospun Nanofibers in Biomedical Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Extracellular vesicles in disease
- Atherosclerosis and Cardiovascular Diseases
- Infective Endocarditis Diagnosis and Management
- Clusterin in disease pathology
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Orthopaedic implants and arthroplasty
- Congenital heart defects research
- Caveolin-1 and cellular processes
- Cell Adhesion Molecules Research
- Cardiac and Coronary Surgery Techniques
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Lipoproteins and Cardiovascular Health
- Infectious Aortic and Vascular Conditions
Kemerovo State University
2020-2025
Czech Academy of Sciences
2012
Czech Academy of Sciences, Institute of Molecular Genetics
2012
Calciprotein particles (CPPs), which increasingly arise in the circulation during disorders of mineral homeostasis, represent a double-edged sword protecting human organism from extraskeletal calcification but potentially causing endothelial dysfunction. Existing models, however, failed to demonstrate detrimental action CPPs on cells (ECs) under flow. Here, we applied flow culture system, where arterial ECs were co-incubated with for 4 h, and normolipidemic normotensive rat model (10 daily...
An association between high serum calcium/phosphate and cardiovascular events or death is well-established. However, a mechanistic explanation of this correlation lacking. Here, we examined the role calciprotein particles (CPPs), nanoscale bodies forming in human blood upon its supersaturation with calcium phosphate, disease. The patients coronary artery disease cerebrovascular displayed an increased propensity to form CPPs combination elevated ionised as well reduced albumin levels,...
Calciprotein particles (CPPs) represent an inherent mineral buffering system responsible for the scavenging of excessive Ca2+ and PO43− ions in order to prevent extraskeletal calcification, although contributing development endothelial dysfunction during circulation bloodstream. Here, we performed label-free proteomic profiling identify functional consequences CPP internalisation by cells (ECs) found molecular signatures significant disturbances mitochondrial lysosomal physiology, including...
Current techniques for the detection of vasa vasorum (VV) in vascular pathology include staining endothelial cell (EC) markers such as CD31 or VE-cadherin. However, this approach does not permit an objective assessment geometry upon vasospasm and clinical relevance specification found developmental biology studies remains unclear. Here, we performed a combined immunostaining rat abdominal aorta (rAA) human saphenous vein (hSV) various EC smooth muscle (VSMC) that latter (e.g., alpha actin...
The lack of suitable autologous grafts and the impossibility using synthetic prostheses for small artery reconstruction make it necessary to develop alternative efficient vascular grafts. In this study, we fabricated an electrospun biodegradable poly(ε-caprolactone) (PCL) prosthesis poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) (PHBV/PCL) loaded with iloprost (a prostacyclin analog) as antithrombotic drug cationic amphiphile antibacterial activity. were characterized in...
Major adverse cardiovascular events occurring upon coronary artery bypass graft surgery are typically accompanied by endothelial dysfunction. Total arterial revascularisation, which employs both left and right internal thoracic arteries instead of the saphenous vein to create a bypass, is associated with better mid- long-term outcomes. We suggested that molecular profiles human cells (HCAECs) mammary (HITAECs) coherent in terms transcriptomic proteomic signatures, were then investigated RNA...
Here, we examined the expression of ceramide metabolism enzymes in subcutaneous adipose tissue (SAT), epicardial (EAT) and perivascular (PVAT) 30 patients with coronary artery disease (CAD) valvular heart (VHD) by means quantitative polymerase chain reaction fluorescent Western blotting. The EAT CAD showed higher genes responsible for biosynthesis (SPTLC1, SPTLC2, CERS1, 5, 6, DEGS1, SMPD1) utilization (ASAH1, SGMS1). PVAT was characterized mRNA levels CERS3, CERS4, SMPD1, enzyme (SGMS2). In...
Calciprotein particles (CPPs) are indispensable scavengers of excessive Ca2+ and PO43− ions in blood, being internalised recycled by liver spleen macrophages, monocytes, endothelial cells (ECs). Here, we performed a pathway enrichment analysis cellular compartment-specific proteomes primary human coronary artery ECs (HCAEC) internal thoracic (HITAEC) treated with (amorphous) or secondary (crystalline) CPPs (CPP-P CPPs, respectively). Exposure to CPP-P CPP-S induced notable upregulation of:...
Implementation of small-diameter tissue-engineered vascular grafts (TEVGs) into clinical practice is still delayed due to the frequent complications, including thrombosis, aneurysms, neointimal hyperplasia, calcification, atherosclerosis, and infection. Here, we conjugated a vasodilator/platelet inhibitor, iloprost, an antimicrobial cationic amphiphilic drug, 1,5-bis-(4-tetradecyl-1,4-diazoniabicyclo [2.2.2]octan-1-yl) pentane tetrabromide, luminal surface electrospun poly(ε-caprolactone)...
Mitomycin C (MMC)-induced genotoxic stress can be considered to a novel trigger of endothelial dysfunction and atherosclerosis—a leading cause cardiovascular morbidity mortality worldwide. Given the increasing load on human organism, decryption molecular pathways underlying stress-induced could improve our understanding role in atherogenesis. Here, we performed proteomic profiling coronary artery cells (HCAECs) internal thoracic (HITAECs) vitro that were exposed MMC identify biochemical...
Although saphenous veins (SVs) are commonly used as conduits for coronary artery bypass grafting (CABG), internal thoracic (ITA) grafts have significantly higher long-term patency. As SVs and ITA endothelial cells (ECs) a considerable level of heterogeneity, we suggested that synergistic paracrine interactions between CA ECs (HCAECs HITAECs, respectively) may explain the increased resistance adjacent CAs to atherosclerosis restenosis. In this study, measured gene protein expression molecules...
Background Whereas the risk factors for structural valve degeneration (SVD) of glutaraldehyde-treated bioprosthetic heart valves (BHVs) are well studied, those responsible failure BHVs fixed with alternative next-generation chemicals remain largely unknown. This study aimed to investigate reasons behind development SVD in ethylene glycol diglycidyl ether-treated BHVs. Methods and Results Ten excised because SVD, 5 calcified aortic (AVs) replaced calcific AV disease were collected their...
Highlights . Spontaneous endothelial-to-mesenchymal transition of primary human umbilical vein endothelial cells (HUVEC) is characterized by an acquired expression SNAI2 and TWIST1 genes, loss markers transcription factors (CD31/PECAM1, VE-cadherin, ERG factor), pronounced S100A4 ACTA2 active production type I collagen, a major component the extracellular matrix. An optimal algorithm to detect includes gene profiling lineage ( PECAM1, CDH5, VWF, ), factors, mesenchymal specification FAP,...
We have investigated expression of skeletal calsequestrin (CSQ1) and fiber type composition in normal regenerated fast slow muscles the left heart ventricles euthyroid (EU), hypothyroid (HY) hyperthyroid (TH) adult inbred Lewis strain rats. The CSQ1 level was determined by SDS-PAGE followed Western blot analysis. gene assessed using reverse transcription subsequent real time polymerase chain reaction. Muscle regeneration achieved intramuscular grafting either soleus or extensor digitorum...
Highlights When modeling mineral stress, a closed biochemical system shows the following calcium distribution ratio among compartments: freely circulating ions (Ca2+) – 50%, bound to albumin (CPM) - 20%, and phosphorus (CPC) 30%. The of ionized protein- or phosphorus-bound was 1:1 corresponded physiological in blood, indicating relevance simulations performed. Even under supraphysiological natural depots (CPM HRC) maintain calcium-binding capacity at level (50%), relative CPM HRC conditions...
Highlights To achieve a 10% (i.e., 1.1-fold) increase in ionized calcium level the serum-free cell culture medium and Wistar rat serum, 10 µg/mL (e.g. with CaCl 2 ) should be added; this corresponds to upper quartile of human population. Incubation or calciprotein monomers (10 calcium) does not induce any pathological effects primary arterial endothelial cells, although are internalised by cells similar particles. coronary artery particles triggers an increased expression VCAM1 , ICAM1 SELE...
Background: Citrullination represents a post-translational modification primarily mediated by peptidylarginine deiminase (PADI) 2 and 4 resulting in the conversion of positively charged to neutrally peptidylcitrulline. Molecular consequences citrullination include generation neoepitopes which provoke production autoantibodies implicated development autoimmune diseases. As initiates, promotes, is enhanced aseptic inflammation plays pivotal role atherosclerosis, we proposed that might...
Calciprotein particles (CPPs) are essential circulating scavengers of excessive Ca