A5046861308- Microbial Community Ecology and Physiology
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Gut microbiota and health
- Entomopathogenic Microorganisms in Pest Control
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Advanced biosensing and bioanalysis techniques
- Legume Nitrogen Fixing Symbiosis
- DNA and Nucleic Acid Chemistry
- Methane Hydrates and Related Phenomena
- Epigenetics and DNA Methylation
- Viral Infections and Vectors
- Multiple Sclerosis Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Environmental DNA in Biodiversity Studies
- Indoor Air Quality and Microbial Exposure
- Influenza Virus Research Studies
- Cancer-related molecular mechanisms research
- Insect and Pesticide Research
- Plant Pathogenic Bacteria Studies
- Virology and Viral Diseases
Siberian Branch of the Russian Academy of Sciences
2016-2025
Institute of Chemical Biology and Fundamental Medicine
2016-2025
Russian Academy of Sciences
2002-2024
Lomonosov Moscow State University
2017
Novosibirsk State University
2011
Institute of Cytology and Genetics
2003-2004
Institute of Bioorganic Chemistry
2003
We report here the ~670 Mb genome assembly of Asian seabass (Lates calcarifer), a tropical marine teleost. used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive chromosome-level contig N50 size over 1 scaffold 25 that span ~90% genome. The population structure L. calcarifer complex was analyzed re-sequencing 61 individuals representing various regions across species’ native range. SNP analyses...
The emergence of novel avian influenza viruses in migratory birds is concern because the potential for virus dissemination during fall migration. We report identification highly pathogenic subtype H5N8, clade 2.3.4.4, and their reassortment with other waterfowl shorebirds Siberia.
Abstract The Asian arowana ( Scleropages formosus ), one of the world’s most expensive cultivated ornamental fishes, is an endangered species. It represents ancient lineage teleosts: Osteoglossomorpha. Here, we provide a high-quality chromosome-level reference genome female golden-variety using combination deep shotgun sequencing and high-resolution linkage mapping. In addition, have also generated two draft assemblies for red green varieties. Phylogenomic analysis supports sister group...
Microevolutionary mechanisms of resistance to a bacterial pathogen were explored in population the Greater wax moth, Galleria mellonella, selected for an 8.8-fold increased against entomopathogenic bacterium Bacillus thuringiensis (Bt) compared with non-selected (suspectible) line. Defense strategies resistant and susceptible insect lines uncover underpinning resistance, possible cost those survival strategies. In uninfected state, insects exhibited enhanced basal expression genes related...
Abstract Background Gut microbiota has been increasingly acknowledged to shape significantly human health, contributing various autoimmune diseases, both intestinal and non-intestinal, including multiple sclerosis (MS). studies in patients with relapsing remitting MS strongly suggested its possible role immunoregulation; however, the profile potential of gut involvement primary progressive (PPMS) received much less attention due rarity this disease form. We compared composition structure...
The aim of this work was to study the gut microbial diversity from eight species wild fish with different feeding habits, digestive physiology (gastric vs agastric) and provide comparative structural analysis communities within their environment (food items, water, sediments macrophytes).
Abstract Gut bacteria influence the development of different pathologies caused by bacteria, fungi and parasitoids in insects. Wax moth larvae became more susceptible to fungal infections after envenomation ectoparasitoid Habrobracon hebetor . In addition, spontaneous bacterioses occurred often envenomated larvae. We analyzed alterations midgut microbiota immunity wax response H. topical infection ( Beauveria bassiana ) alone or combination using 16S rRNA sequencing, an analysis cultivable a...
Endothelial colony-forming cells (ECFC) are currently considered as a promising cell population for the pre-endothelialization or pre-vascularization of tissue-engineered constructs, including small-diameter biodegradable vascular grafts. However, extent heterogeneity between ECFC and mature endothelial (EC) is unclear. Here, we performed transcriptome-wide study to compare gene expression profiles ECFC, human coronary artery (HCAEC), umbilical vein (HUVEC). Characterization abovementioned...
The aim of this study was to evaluate, via various molecular methods, the possible correlations between microbial community structure Prussian carp and environmental compartments their habitat.Microbial communities in intestine were studied using PCR-screening, cloning next-generation high-throughput sequencing 16S ribosomal RNA genes. rDNA metagenomic showed higher bacterial diversity comparison with clone libraries, while group-specific PCR positive detection nine bacteria phyla....
Multiple sclerosis (MS) is a severe neurodegenerative disease of polygenic etiology affecting the central nervous system. In addition to genetic factors, epigenetic mechanisms, primarily DNA methylation, which regulate gene expression, play an important role in MS development and progression. this study, we have performed first whole-genome methylation profiling peripheral blood mononuclear cells relapsing-remitting (RRMS) primary-progressive (PPMS) patients compared them those healthy...
Ixodes persulcatus, pavlovskyi, and Dermacentor reticulatus ticks inhabiting Western Siberia are responsible for the transmission of a number etiological agents that cause human animal tick-borne diseases. Because these abundant in suburbs large cities, agricultural areas, popular tourist sites frequently attack people livestock, data regarding microbiomes organisms required. Using metagenomic 16S profiling, we evaluate bacterial communities associated with I. D. collected from Novosibirsk...
Exosomes represent a sort of extracellular vesicles, which transfer molecular signals in organism and possess markers producing cells. Our study was aimed at search exosomes the tears healthy humans, confirmation their nature examination exosome morphological molecular-biological characteristics. The (110-340 ml) were collected from 24 donors (aged 46-60 years); individual probes centrifuged 20000 g for 15 min to pellet cell debris. supernatants examined electron microscope using negative...
Abstract First triplets of mRNA coding region affect the yield translation. We have applied flowseq method to analyze >30 000 variants codons 2–11 fluorescent protein reporter identify factors affecting synthesis. While negative influence secondary structure on translation has been confirmed, a positive role rare at beginning sequence for gene expression not observed. The identity proximal start codon contributes more then distant ones. Additional in-frame enhance translation, while...
This study describes two viral communities from the world’s oldest lake, Lake Baikal. For analysis, we chose under-ice and late spring periods of year as most productive for These show maximum seasonal biomass phytoplankton bacterioplankton, which are targets viruses, including bacteriophages. At that time, main group viruses were tailed bacteriophages order Caudovirales belong to families Myoviridae, Siphoviridae Podoviridae. Annotation functional genes revealed during period, “Phages,...
Abstract Fungal infections and toxicoses caused by insecticides may alter microbial communities immune responses in the insect gut. We investigated effects of Metarhizium robertsii fungus avermectins on midgut physiology Colorado potato beetle larvae. analyzed changes bacterial community, immunity- stress-related gene expression, reactive oxygen species (ROS) production, detoxification enzyme activity response to topical infection with M. fungus, oral administration , a combination two...
Gut physiology and the bacterial community play crucial roles in insect susceptibility to infections insecticides. Interactions among Colorado potato beetle Leptinotarsa decemlineata (Say), its associates, pathogens xenobiotics have been insufficiently studied. In this paper, we present our study of survival, midgut histopathology, activity digestive enzymes communities L . larvae under influence Bacillus thuringiensis var. tenebrionis ( morrissoni ) Bt ), a natural complex avermectins...
Ribosomal protein uS10, a product of the RPS20 gene, is an essential constituent small (40S) subunit human ribosome. Disruptive mutations in its gene are associated with predisposition to hereditary colorectal carcinoma. Here, using HEK293T cells, we show that deficiency this leads decrease level ribosomes (ribosomal shortage). RNA sequencing total and polysome-associated mRNA samples reveals hundreds genes differentially expressed transcriptome (t)DEGs translatome (p)DEGs under conditions...
Background: Infective endocarditis (IE) is an infectious disease caused by the hematogenous dissemination of bacteria into heart valves. Improving identification pathogens that cause IE important to increase effectiveness its therapy and reduce mortality this pathology. Methods: Ten native valves obtained from patients undergoing valve replacements were analyzed. Bacterial invasion in was studied Gram staining histological sections. Histopathological changes accompanied with bacterial...
This brief review focuses on the properties of bioaerosols, presenting some recent results metagenomic studies air microbiome performed using next-generation sequencing. The taxonomic composition and structure bioaerosol may display diurnal seasonal dynamics be dependent meteorological events such as dust storms, showers, fogs, etc., well pollution. Proteobacteria Ascomycota members are common dominants in bioaerosols different troposphere layers. microbiological lower affects diversity...