A5063168910- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Bacteriophages and microbial interactions
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Viral gastroenteritis research and epidemiology
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoparticles: synthesis and applications
- DNA and Nucleic Acid Chemistry
- Antimicrobial Peptides and Activities
- Poxvirus research and outbreaks
- Animal Virus Infections Studies
- Genomics and Phylogenetic Studies
- Hepatitis B Virus Studies
- Microbial infections and disease research
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- RNA and protein synthesis mechanisms
- Circular RNAs in diseases
- Advanced Nanomaterials in Catalysis
- Respiratory viral infections research
Institute of Chemical Biology and Fundamental Medicine
2015-2024
Siberian Branch of the Russian Academy of Sciences
2015-2024
Novosibirsk State University
2010-2023
Russian Academy of Sciences
2008-2021
Novosibirsk State Pedagogical University
2007-2016
Ulyanovsk Regional Oncology Center
2015
State Research Center of Virology and Biotechnology VECTOR
1999-2011
Research Institute of Human Morphology
1997
Academy of Medical Sciences
1997
Recent studies suggest that extracellular vesicles may be the key to timely diagnosis and monitoring of genito-urological malignancies. In this study we investigated composition content found in urine healthy donors prostate cancer patients. Urine 14 PCa patients 20 volunteers was clarified by low-speed centrifugation total fraction obtain high-speed centrifugation. The exosome-enriched obtained filtration through a 0.1 μm pore filter. Transmission electron microscopy showed cell-free both...
Virus reproduction and the time course of changes in liver kidney functions blood clotting system were studied visceral organs green monkeys baboons infected with Ebola virus (subtype Zaire). It was shown that monocytes macrophages first cells to be virus, followed by hepatocytes, adrenocorticocytes, fibroblasts, endotheliocytes. The early late pathologic monkey are described. Biochemical data on infection presented. responses vascular permeability species specific: Fibrin deposited vessels...
ABSTRACT A hallmark of Ebola virus (EBOV) infection is the formation viral inclusions in cytoplasm infected cells. These contain EBOV nucleocapsids and are sites replication nucleocapsid maturation. Although there growing evidence that create a protected environment fosters replication, little known about their role host response to infection. The cellular stress an effective antiviral strategy leads granule (SG) translational arrest mediated by phosphorylation translation initiation factor,...
The International Committee on Taxonomy of Viruses (ICTV) organizes the classification viruses into taxa, but is not responsible for nomenclature taxa members. experts groups, such as ICTV Study Groups, recommend and naming their strains, variants, isolates. Filoviridae Group has recently introduced an updated filoviruses. Subsequently, together with numerous other filovirus experts, a consistent natural genetic variants isolates was developed that aims at simplifying retrieval sequence data...
Human peripheral blood contains RNA in cells and extracellular membrane vesicles, microvesicles exosomes, as well cell-free ribonucleoproteins. Circulating mRNAs noncoding RNAs, being internalized, possess the ability to modulate vital processes recipient cells. In this study, with SOLiD sequencing technology, we performed identification, classification, quantification of RNAs from fractions: cells, plasma, plasma vesicles pelleted at 16,000<mml:math...
Exosomes are 40–100 nm nanovesicles containing RNA and different proteins. proteins, lipids, mRNAs, microRNAs important in intracellular communication immune function. from sources usually obtained by combination of centrifugation ultracentrifugation according to published data can contain a few dozens thousands Crude exosome preparations milk eighteen horses were for the first time using several standard centrifugations. Exosome additionally purified FPLC gel filtration. Individual...
ABSTRACT HeLa cells expressing the recombinant Marburg virus (MBGV) nucleoprotein (NP) have been studied by immunoelectron microscopy. It was found that MBGV NPs assembled into large aggregates which were in close association with membranes of rough endoplasmic reticulum. Further analysis these revealed formed tubule-like structures arranged a hexagonal pattern. A similar pattern preformed nucleocapsids detected intracellular inclusions induced infection. Our data indicated NP is able to...
The aim of our study was to attract the attention researchers at problem contamination exosome preparations. Using a transmission electron microscope JEM-1400 ("JEOL", Japan) we have examined preparations, isolated according conventional scheme sequential centrifugation from different biological fluids: plasma and urine healthy persons patients with oncologic diseases, bovine serum, culture fluid (MDCK, MDA-MB и MCF-7 cells). All preparations (over 200) contained exosomes, which were...
Exosomes represent a sort of extracellular vesicles, which transfer molecular signals in organism and possess markers producing cells. Our study was aimed at search exosomes the tears healthy humans, confirmation their nature examination exosome morphological molecular-biological characteristics. The (110-340 ml) were collected from 24 donors (aged 46-60 years); individual probes centrifuged 20000 g for 15 min to pellet cell debris. supernatants examined electron microscope using negative...
Exosomes are nanovesicles (30-100 nm) containing various RNAs and different proteins. important in intracellular communication, immune function, etc. from sources including placenta were mainly obtained by types of centrifugation ultracentrifugations reported to contain a few dozen thousands First crude exosome preparations four placentas (normal pregnancy) here using several standard centrifugations but then additionally purified gel filtration on Sepharose 4B. Individual demonstrated...
Cold atmospheric plasma (CAP) is an intensively-studied approach for the treatment of malignant neoplasms. Various active oxygen and nitrogen compounds are believed to be main cytotoxic effectors on biotargets; however, comprehensive mechanism CAP interaction with living cells tissues remains elusive. In this study, we experimentally determined optimal discharge regime (or semi-selective regime) direct jet cancer cells, under which lung adenocarcinoma A549, A427 NCI-H23 demonstrated...
Virus budding from the basolateral domain of infected polarized cells could be one mechanisms underlying quick systemic infection induced by Marburg virus (MARV). We found that MARV buds pole hepatocytes and bile epithelial in guinea pigs, which leads to release infectious into vascular system. Basolateral might orchestrated basolaterally located matrix protein VP40, induces a partial relocalization glycoprotein apical plasma membrane. This redistribution is prerequisite for virions domain.