A5044605930- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Utilization and Effects
- Plant Virus Research Studies
- Insect and Arachnid Ecology and Behavior
- Invertebrate Immune Response Mechanisms
- Insect-Plant Interactions and Control
- Mosquito-borne diseases and control
- Nanoparticles: synthesis and applications
- Aquaculture disease management and microbiota
- Viral Infections and Immunology Research
- Nematode management and characterization studies
- Gut microbiota and health
- Neurobiology and Insect Physiology Research
- Recycling and Waste Management Techniques
- thermodynamics and calorimetric analyses
- Myxozoan Parasites in Aquatic Species
- Microplastics and Plastic Pollution
- Aquaculture Nutrition and Growth
- Insect behavior and control techniques
- Aquatic life and conservation
Institute of Systematics and Ecology of Animals
2016-2025
Siberian Branch of the Russian Academy of Sciences
2024
Russian Academy of Sciences
2013
Abstract Gut bacteria influence the development of different pathologies caused by bacteria, fungi and parasitoids in insects. Wax moth larvae became more susceptible to fungal infections after envenomation ectoparasitoid Habrobracon hebetor . In addition, spontaneous bacterioses occurred often envenomated larvae. We analyzed alterations midgut microbiota immunity wax response H. topical infection ( Beauveria bassiana ) alone or combination using 16S rRNA sequencing, an analysis cultivable a...
Abstract Fungal infections and toxicoses caused by insecticides may alter microbial communities immune responses in the insect gut. We investigated effects of Metarhizium robertsii fungus avermectins on midgut physiology Colorado potato beetle larvae. analyzed changes bacterial community, immunity- stress-related gene expression, reactive oxygen species (ROS) production, detoxification enzyme activity response to topical infection with M. fungus, oral administration , a combination two...
Gut physiology and the bacterial community play crucial roles in insect susceptibility to infections insecticides. Interactions among Colorado potato beetle Leptinotarsa decemlineata (Say), its associates, pathogens xenobiotics have been insufficiently studied. In this paper, we present our study of survival, midgut histopathology, activity digestive enzymes communities L . larvae under influence Bacillus thuringiensis var. tenebrionis ( morrissoni ) Bt ), a natural complex avermectins...
Abstract The effect of Wolbachia on the viability and antimicrobial activity ectoparasitoid Habrobracon hebetor was evaluated in laboratory experiments. Two lines parasitoid, -infected (W+) -free (W−), were used. Parasitoid larvae fed with a host orally infected sublethal dose Bacillus thuringiensis (Bt) uninfected Bt. survival assessed at developmental stages from second-instar to adults. At all stages, there no statistically significant differences between W+ W−, regardless Bt infection....
Combination of insect pathogenic fungi and microbial metabolites is a prospective method for mosquito control. The effect the entomopathogenic fungus Metarhizium robertsii J.F. Bischoff, S.A. Rehner & Humber avermectins on survival physiological parameters Aedes aegypti (Linnaeus, 1762) larvae (dopamine concentration, glutathione S-transferase (GST), nonspecific esterases (EST), acid proteases, lysozyme-like, phenoloxidase (PO) activities) was studied. It shown that combination these agents...
We assume that certain representatives of gut microflora mediate immune changes during dysbiosis, accelerating septicemia caused by Bacillus thuringiensis.Co-introduction Citrobacter freundii with thuringiensis var. tenebrionis (morrisoni) (Bt) led to an increase in Colorado potato beetle (CPB) larval mortality 69.0% (1.3-5×) and a synergistic effect was observed from day 1 6. Ultrathin sections the CPB midgut showed autophagosome formation partial destruction microvilli under influence Bt,...
Various insect bacterial associates are involved in pathogeneses caused by entomopathogenic fungi. The outcome of infection (fungal growth or decomposition) may depend on environmental factors such as temperature. aim this study was to analyze the communities and immune response Galleria mellonella larvae injected with Cordyceps militaris incubated at 15 °C 25 °C. We examined changes CFUs, (Illumina MiSeq 16S rRNA gene sequencing) expression immune, apoptosis, ROS stress-related genes (qPCR)...
The aim of the study was to investigate skin microbiota Prussian carp infested by ectoparasites from genera Argulus and Lernaea.Associated were investigated sequencing V3, V4 hypervariable regions 16S rRNA using Illumina MiSeq platform.According Spearman rank correlation test, increasing load ulcerations weakly negatively correlated with reduction in abundance following taxa: Acrobacter, bacteria C39 (Rhodocyclaceae), Rheinheimera, Comamonadaceae, Helicobacteraceae Vogesella. In this study,...
For the first time causes of mass death embryos in a population Lymantria dispar (Linnaeus, 1758) with biochemical and molecular methods are investigated. In this study egg masses were collected territory Russia from two populations, low (the West Siberian population) high (Trans-Ural) density analyzed. Spring hatching larvae eggs stored under constant laboratory conditions at 2º C was times lower for insects Trans-Ural (TU) compared to (WS) (43 ± 6 % 86 7 accordingly). The level virus...
Infection of intestinal tissues with Wolbachia has been found in Habrobracon hebetor. There are not many studies on the relationship between and Wolbachia, they focus predominantly sex index an infected parasitoid, its fertility, behavior. The actual role biology is yet clear. method complete eradication parasitoid was developed here, effects endosymbiont host's digestive metabolism were compared two lines (Wolbachia-positive Wolbachia-negative). In gut Wolbachia+ larvae, lipases' activity...
The Colorado potato beetle (CPB) is one of the most serious insect pests due to its high ecological plasticity and ability rapidly develop resistance insecticides. use biological insecticides based on viruses a promising approach control pests, but information which infect leaf feeding beetles scarce. We performed metagenomic analysis 297 CPB genomic transcriptomic samples from public National Center for Biotechnology Information Sequence Read Archive (NCBI SRA) database. reads that were not...
Entomopathogenic fungi can be inhibited by different soil microorganisms, but the effect of a microbiota on fungal growth, survival, and infectivity toward insects is insufficiently understood. We investigated level fungistasis Metarhizium robertsii Beauveria bassiana in soils conventional potato fields kitchen gardens. Agar diffusion methods, 16S rDNA metabarcoding, bacterial DNA quantification, assays Leptinotarsa decemlineata survival inoculated with conidia were used. Soils gardens...
Entomopathogenic fungi may interact with insects' symbiotic bacteria during infection. We hypothesized that topical infection Beauveria bassiana alter the microbiota of Colorado potato beetle (CPB) and these modifications course mycoses. used a model two concentrations conidia: (1) high concentration causes rapid (acute) pathogenesis fast mortality followed by bacterial decomposition insects; (2) lower leads to prolonged ending in conidiation on cadavers. The fungal infections increased...
Abstract Ectoparasitoids may change host metabolism, making the environment more favorable for development of their larvae. These alterations also be suitable growth and proliferation commensal microorganisms pathogens. This is especially important in relation to cuticular microbiota, with which ectoparasitoids interact directly. However, biochemical microbiological changes occurring on insect cuticles response parasitoid venoms are insufficiently understood. We assayed larvae wax moth,...
Invasion of microorganisms into the gut insects triggers a cascade immune reactions accompanied by increased synthesis effectors (such as antimicrobial peptides, cytokines, and amino acids), leading to changes in physiological state host. We hypothesized that even an inactivated bacterium can induce response insect. The aim this study was compare roles reactive oxygen species (ROS) formation detoxification antioxidant systems Colorado potato beetle (CPB) larval model first hours after...