A5014348143- Electrospun Nanofibers in Biomedical Applications
- Cardiac Valve Diseases and Treatments
- Orthopaedic implants and arthroplasty
- Infective Endocarditis Diagnosis and Management
- Tissue Engineering and Regenerative Medicine
- Cardiac and Coronary Surgery Techniques
- Bone Tissue Engineering Materials
- Aortic Disease and Treatment Approaches
- Polymer Surface Interaction Studies
- Infectious Aortic and Vascular Conditions
- biodegradable polymer synthesis and properties
- Cardiac Structural Anomalies and Repair
- Intracranial Aneurysms: Treatment and Complications
- Aortic aneurysm repair treatments
- Engineering Technology and Methodologies
- Parathyroid Disorders and Treatments
- Silk-based biomaterials and applications
- Orthopedic Infections and Treatments
- Hemostasis and retained surgical items
- Periodontal Regeneration and Treatments
- Cerebrovascular and Carotid Artery Diseases
- Surgical Sutures and Adhesives
- Additive Manufacturing and 3D Printing Technologies
- Blood properties and coagulation
- Coronary Interventions and Diagnostics
Kemerovo State University
2015-2024
Research Institute of Complex Problems of Cardiovascular Disease Russian Academy of Medical Sciences
2014-2024
Institut de Recherche pour le Développement
2022
Institute of Experimental Physics of the Slovak Academy of Sciences
2018
Kemerovo Cardiology Center
2016
Russian Academy of Sciences
2015
Abstract Calcium phosphate bions (CPB) are biomimetic mineralo-organic nanoparticles which represent a physiological mechanism regulating the function, transport and disposal of calcium phosphorus in human body. We hypothesised that CPB may be pathogenic entities even cause cardiovascular calcification. Here we revealed isolated from calcified atherosclerotic plaques artificially synthesised morphologically chemically indistinguishable entities. Their formation is accelerated along with...
Abstract Polymeric heart valves seem to be an attractive alternative mechanical and biological prostheses as they are more durable, due the superior properties of novel polymers, have biocompatibility hemodynamics comparable tissue substitutes. This study reports a comprehensive assessment nanocomposite based on functionalised graphene oxide poly(carbonate-urea)urethane with trade name “Hastalex” in comparison GORE-TEX, commercial polymer routinely used for cardiovascular medical devices....
Atherosclerosis, calcific aortic valve disease (CAVD), and bioprosthetic heart degeneration (alternatively termed structural deterioration, SVD) represent three diseases affecting distinct components of the circulatory system their substitutes, yet sharing multiple risk factors commonly leading to extraskeletal calcification. Whereas histopathology mentioned disorders is well-described, ultrastructural pathology largely obscure due lack appropriate investigation techniques. Employing an...
The blend of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) has recently been considered promising for vascular tissue engineering. However, it was shown that PHBV/PCL grafts require biofunctionalization to achieve high primary patency rate. Here we compared immobilization arginine–glycine–aspartic acid (RGD)-containing peptides the incorporation endothelial growth factor (VEGF) as two widely established approaches. Electrospun small-diameter with either...
Small diameter arterial bypass grafts are considered as unmet clinical need since the current have poor patency of 25% within 5 years. We developed a 3D scaffold manufactured from natural and synthetic biodegradable polymers, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) poly(ε-caprolactone) (PCL), respectively. Further to improve biophysical properties well endothelialisation, were covalently conjugated with arginine-glycine-aspartic acid (RGD) bioactive peptides. The...
Calciprotein particles (CPPs), which increasingly arise in the circulation during disorders of mineral homeostasis, represent a double-edged sword protecting human organism from extraskeletal calcification but potentially causing endothelial dysfunction. Existing models, however, failed to demonstrate detrimental action CPPs on cells (ECs) under flow. Here, we applied flow culture system, where arterial ECs were co-incubated with for 4 h, and normolipidemic normotensive rat model (10 daily...
An association between high serum calcium/phosphate and cardiovascular events or death is well-established. However, a mechanistic explanation of this correlation lacking. Here, we examined the role calciprotein particles (CPPs), nanoscale bodies forming in human blood upon its supersaturation with calcium phosphate, disease. The patients coronary artery disease cerebrovascular displayed an increased propensity to form CPPs combination elevated ionised as well reduced albumin levels,...
The combination of a natural hydrophilic polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and synthetic hydrophobic poly(ε-caprolactone) (PCL) is promising for the preparation biodegradable biocompatible small-diameter vascular grafts bypass surgery. However, physico-mechanical properties endothelialization rate PHBV/PCL are poor. We suggested that incorporation endothelial growth factor (VEGF) into may improve their enhance endothelialization. Here we compared morphology,...
The lack of suitable autologous grafts and the impossibility using synthetic prostheses for small artery reconstruction make it necessary to develop alternative efficient vascular grafts. In this study, we fabricated an electrospun biodegradable poly(ε-caprolactone) (PCL) prosthesis poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) (PHBV/PCL) loaded with iloprost (a prostacyclin analog) as antithrombotic drug cationic amphiphile antibacterial activity. were characterized in...
Modification with Arg-Gly-Asp (RGD) peptides is a promising approach to improve biocompatibility of small-calibre vascular grafts but it unknown how different RGD sequence composition impacts graft performance. Here we manufactured 1.5 mm poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) modified by distinct linear or cyclic immobilized short long amine linker arms. Modified prostheses were tested in vitro assess their mechanical properties, hemocompatibility, thrombogenicity...
Superior polymers represent a promising alternative to mechanical and biological materials commonly used for manufacturing artificial heart valves. The study is aimed at assessing poly(styrene-block-isobutylene-block-styrene) (SIBS) properties comparing them with polytetrafluoroethylene (Gore-texTM, reference sample). Surface topography of both was evaluated scanning electron microscopy atomic force microscopy. were measured under uniaxial tension. water contact angle estimated evaluate...
In this study, we incorporated carbon nanotubes (CNTs) into poly(styrene-block-isobutylene-block-styrene) (SIBS) to investigate the physical characteristics of resulting nanocomposite and its cytotoxicity endothelial cells. CNTs were dispersed in chloroform using sonication following addition a SIBS solution at different ratios. The resultant films analyzed by X-ray microtomography, optical scanning electron microscopy; tensile strength was examined uniaxial tension testing; hydrophobicity...
Currently, an ultrastructural analysis of cardiovascular tissues is significantly complicated. Routine histopathological examinations and immunohistochemical staining suffer from a relatively low resolution light microscopy, whereas the fluorescence imaging plaques bioprosthetic heart valves yields considerable background noise convoluted extracellular matrix that often results in signal-to-noise ratio. Besides, sectioning calcified or stent-expanded blood vessels mineralised leads to...
Tissue-engineered vascular graft for the reconstruction of small arteries is still an unmet clinical need, despite fact that a number promising prototypes have entered preclinical development. Here we test Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)Poly(ε-caprolactone) 4-mm-diameter grafts equipped with endothelial growth factor (VEGF), basic fibroblast (bFGF) and stromal cell-derived 1α (SDF-1α) surface coated heparin iloprost (PHBV/PCL[VEGF-bFGF-SDF]Hep/Ilo, n = 8) in sheep carotid artery...
Background Currently, the search for bioactive molecules capable of promoting formation vascular tissue is still ongoing. We have previously demonstrated that incorporation growth factors and chemoattractant into biodegradable tubular scaffolds can increase their primary patency upon implantation rat abdominal aorta. However, further studies are required to investigate remodeling using functionalized grafts with same diameter as a replaced native vessel. Aim To specific aspects de novo...
Tissue-engineered vascular grafts are widely tested as a promising substitute for both arterial bypass and replacement surgery. We previously demonstrated that incorporation of VEGF into electrospun tubular scaffolds from poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) enhances formation an endothelial cell monolayer. However, overdose can induce tumor-like vasculature; thereby, other bioactive factors needed to support VEGF-driven endothelialization successful recruitment...
Nanocomposites based on poly(styrene-block-isobutylene-block-styrene) (SIBS) and single-walled carbon nanotubes (CNTs) were prepared characterized in terms of tensile strength as well bio- hemocompatibility. It was shown that modification CNTs using dodecylamine (DDA), featured by a long non-polar alkane chain, provided much better dispersion SIBS compared to unmodified CNTs. As result such modification, the nanocomposite with low molecular weight (Mn = 40,000 g mol-1) containing 4%...
The study analysed the mechanical properties by uniaxial test of porcine and bovine pericardial specimens, stabilised with glutaraldehyde or ethylene glycol diglycidyl ether. Also paper describes, ability these samples compressed to 18 Fr via finite element method. Analysis biomaterial demonstrated a low strength for all types pericardium lowest value EGDE-treated (2.75 N). stress-strain state during simulation leaflet crimping was observed in experimental patch minimum principal logarithmic...
Background Whereas the risk factors for structural valve degeneration (SVD) of glutaraldehyde-treated bioprosthetic heart valves (BHVs) are well studied, those responsible failure BHVs fixed with alternative next-generation chemicals remain largely unknown. This study aimed to investigate reasons behind development SVD in ethylene glycol diglycidyl ether-treated BHVs. Methods and Results Ten excised because SVD, 5 calcified aortic (AVs) replaced calcific AV disease were collected their...
Fibrin is widely used in vascular tissue engineering. Typically, fibrin polymerization initiated by adding exogenous thrombin. In this study, we proposed a protocol for the preparation of completely autologous without use endogenous thrombin and compared properties prepared matrix with that obtained traditional method. Fibrinogen was ethanol precipitation followed either calcium chloride (ExThr), or only (EnThr). We examined structure, mechanical properties, thrombogenicity, degradation rate...