A5025742594- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Synthesis of β-Lactam Compounds
- Cancer therapeutics and mechanisms
- Synthesis and Characterization of Heterocyclic Compounds
- Tissue Engineering and Regenerative Medicine
- Synthesis and biological activity
- CRISPR and Genetic Engineering
- Polymer Synthesis and Characterization
- Nanoplatforms for cancer theranostics
- Viral Infections and Immunology Research
- Antimicrobial agents and applications
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Antimicrobial Peptides and Activities
- Influenza Virus Research Studies
- Synthesis and Biological Evaluation
- Chemical Reactions and Isotopes
- Monoclonal and Polyclonal Antibodies Research
Institute of Chemical Biology and Fundamental Medicine
2013-2024
Siberian Branch of the Russian Academy of Sciences
2012-2024
Bashkir State University
2018-2020
Novosibirsk State University
2010-2019
Academy of Medical Sciences
2011
Russian Academy of Sciences
2008-2011
Institute of Bioorganic Chemistry
2004
Aptamer selection against novel infections is a complicated and time-consuming approach. Synergy can be achieved by using computational methods together with experimental procedures. This study aims to develop reliable methodology for rational aptamer in silico et vitro design. The new approach combines multiple steps: (1) Molecular design, based on screening DNA library directed mutagenesis fit the protein tertiary structure; (2) 3D molecular modeling of target; (3) docking an protein; (4)...
Modification with Arg-Gly-Asp (RGD) peptides is a promising approach to improve biocompatibility of small-calibre vascular grafts but it unknown how different RGD sequence composition impacts graft performance. Here we manufactured 1.5 mm poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) modified by distinct linear or cyclic immobilized short long amine linker arms. Modified prostheses were tested in vitro assess their mechanical properties, hemocompatibility, thrombogenicity...
DNA probes for the studies of damaged strand excision during nucleotide repair (NER) have been designed using novel non-nucleosidic phosphoramidite reagents that contain N-[6-(9-antracenylcarbamoyl)hexanoyl]-3-amino-1,2-propandiol (nAnt) and N-[6-(5(6)-fluoresceinylcarbamoyl)hexanoyl]-3-amino-1,2-propandiol (nFlu) moieties. New lesion-imitating adducts being inserted into show good substrate properties in NER process. Modified extended linear nFlu- nAntr-DNA are suitable estimation specific...
Boron neutron capture therapy is a unique form of adjuvant cancer for various malignancies including malignant gliomas. The conjugation boron compounds and human serum albumin (HSA)-a carrier protein with long plasma half-life-is expected to extend systemic circulation the increase their accumulation in glioma cells. We report on synthesis fluorophore-labeled homocystamide conjugates use thiol-'click' chemistry prepare novel multimodal boronated albumin-based theranostic agents, which could...
The synthesis of new, artificial ribonucleases containing two amino acid residues connected by an aliphatic linker has been developed. Target molecules were synthesized via a catalytic three-component Ugi reaction from diisocyanides. Preliminary investigations proved unspecific nuclease activity the new compounds towards single-stranded RNA and double-stranded circular DNA.
Novel alternatives to traditional antibiotics are now of great demand for the successful treatment microbial infections. Here, we present engineering and properties new oligonucleotide inhibitors RNase P, an essential bacterial enzyme. The series 2'-O-methyl RNA (2'-OMe-RNA) phosphoryl guanidine oligonucleotides were targeted substrate-binding region M1 subunit P. Uniformly modified 2'-OMe selectively possessed good stability in biological media effectively inhibited Their conjugates with...
The purposeful development of synthetic antibacterial compounds requires an understanding the relationship between effects and their chemical structure. This knowledge can be obtained by studying changes in bacteria ultrastructure under action a certain Our study was aimed at examination ultrastructural S. aureus cells caused polycationic amphiphile based on 1,4‒diazabicyclo[2.2.2]octane (DL412), ciprofloxacin hybrid (DL5Cip6); samples were incubated for 15 45 min. DL412 first directly...
Herein, we report a novel strategy to engineer an acid-sensitive anticancer theranostic agent using vector–drug ensemble. The ensemble was synthesized by directly conjugating the linoleic acid (LA)-modified branched polyethyleneimine with chemotherapeutic drug trifluorothymidine. Linoleic residues were grafted onto 25 kDa (PEI) treating PEI chloroanhydride. 5-Trifluoromethyl-2′-deoxyuridine (trifluorothymidine, TFT) introduced into LA-PEI conjugate phosphorylating amidophosphate of...
The tick-borne encephalitis virus (TBEV) is an RNA-containing enveloped virus, which poses a major threat to the well-being and health of humans.In this study, we describe approach inactivation TBEV, involves degradation viral RNA by artificial ribonucleases (aRNases, small organic compounds that exhibit ribonuclease activity in vitro).We demonstrate incubation TBEV with aRNases lead total as indicated plaque formation assay data, but retain immunogenic properties, shown ELISA data.We...
Tissue engineering is a promising area for the production of small-diameter vascular grafts. In recent years, number strategies have been developed to make polymer surfaces prostheses capable selectively adhesion endothelial cells. The arginine–glycine–aspartic acid (RGD) sequence (a cell site that present on many extracellular matrix proteins) target modification. efficiency attachment cells can be influenced both by structure RGD peptide and extent linker group. Aim: determine optimal...
Mammalian nucleotide excision repair (NER), known for its broad substrate specificity, is responsible removal of bulky lesions from DNA. Over 30 proteins are involved in NER, which includes two distinct pathways: global genome NER and transcription-coupled repair. The complexity these processes, the use extended DNA substrates, presence induced by chemotherapy have driven researchers to seek more effective methods assess activity, as well develop model DNAs that serve efficient substrates...