Magnetic nanoparticles (MNPs) have great potential in biochemistry and medical science. In particular, iron oxide demonstrated a promising effect various biomedical applications due to their high magnetic properties, large surface area, stability, easy functionalization. However, colloidal biocompatibility, toxicity of MNPs physiological environments are crucial for vivo application. this context, many research articles focused on the possible procedures coating improve physic-chemical...

Magnetic nanoparticles (MNPs) have great potential in various areas such as medicine, cancer therapy and diagnostics, biosensing, material science. In particular, magnetite (Fe3O4) are extensively used for numerous bioapplications due to their biocompatibility, high saturation magnetization, chemical stability, large surface area, easy functionalization. This paper describes magnetic nanoparticle physical biological properties, emphasizing synthesis approaches, toxicity, biomedical...

A magnetic nanocomposite (MNC) is an integrated nanoplatform that combines a set of functions two types materials. successful combination can give rise to completely new material with unique physical, chemical, and biological properties. The core MNC provides the possibility resonance or particle imaging, field-influenced targeted delivery, hyperthermia, other outstanding applications. Recently, gained attention for external field-guided specific delivery cancer tissue. Further, drug loading...

Pulsed Dipolar Spectroscopy (PDS) methods of Electron Paramagnetic Resonance (EPR) were used to detect and characterize reversible non-covalent dimers Human Serum Albumin (HSA), the most abundant protein in human plasma. The spin labels, MTSL OX063, attached Cys-34 these chemical modifications did affect dimerization HSA, indicating that other post-translational can modulate dimer formation. At physiologically relevant concentrations, HSA does form weak, with a well-defined structure. Dimer...

Abstract Trityl radicals (TAMs) have recently appeared as an alternative source of spin labels for measuring long distances in biological systems. Finland trityl radical (FTAM) served the basis this new generation labels, but FTAM is rather lipophilic and susceptible to self‐aggregation, noncovalent binding with sites proteins, docking at termini duplex DNA. In paper very hydrophilic OX063 TAM low toxicity little tendency aggregation used a label. Human serum albumin (HSA) labeled has...

Magnetic nanoparticles (MNPs) have great potential in the drug delivery area. Iron oxide (Fe3O4) MNPs demonstrated a promising effect due to their ferrimagnetic properties, large surface area, stability, low cost, easy synthesis, and functionalization. Some coating procedures are required improve biocompatibility, decrease toxicity for medical applications. Herein, co-precipitation synthesis of iron coated with four types primary surfactants, polyethylene glycol 2000 (PEG 2000), oleic acid...

Nanoplatforms used for the loading of anticancer drugs constitute a promising approach to cancer treatment and reducing side effects these drugs. Among cutting-edge systems in this area are magnetic nanocomposites (MNCs) nanocapsules (NCs). MNCs considered smart tool magnetic-field-guided targeted drug delivery, resonance imaging, hyperthermia therapy. Nanocapsules offer great potential due their ability control drug-loading capacity, release efficiency, stability, ease with which surfaces...

A series of heteroleptic bipyridine Pd(II) complexes based on 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene (dpp-Bian) or 1,2-bis[(2,4,6-trimethylphenyl)imino]acenaphthene (tmp-Bian) were prepared. All fully characterized by spectrochemical methods, and their crystal structures confirmed X-ray diffraction analysis. The 72 h stability with Bian ligands under physiological conditions was investigated using 1H NMR spectroscopy. anticancer activity all assessed in a panel cancer cell lines...

Detection of the Kirsten rat sarcoma gene (KRAS) mutational status is an important factor for treatment various malignancies. The most common KRAS-activating mutations are caused by single-nucleotide mutations, which usually determined using PCR, allele-specific DNA primers. Oligonucleotide primers with uncharged or partially charged internucleotide phosphate modification have proved their ability to increase sensitivity and specificity single nucleotide mutation detection. To enhance...

In cancer diagnostics, magnetic resonance imaging (MRI) uses contrast agents to enhance the distinction between target tissue and background. Several promising approaches have been developed increase MRI sensitivity, one of which is Overhauser dynamic nuclear polarization (ODNP)-enhanced (OMRI). this study, a macromolecular construct based on human serum albumin nitroxyl radicals (HSA-NIT) was using new synthesis method that significantly increased modification 21 nitroxide residues per...

Magnetic iron oxide (II,III) nanoparticles (MNPs) are highly interested in biomedicine. However, their application is limited by oxidation, aggregation, rapid clearance from the body, and poor biodistribution. Coating human serum albumin (HSA), predominant blood plasma protein, can significantly influence properties, prolong circulation half-life, enhance tumor capture efficiency. Here, we report synthesis of oleic acid Tween20-coated MNPs interaction with HSA. The coating on MNP size, zeta...

Understanding protein-drug complex structures is crucial for elucidating therapeutic mechanisms and side effects. Blind docking facilitates site identification but hindered by computational complexity imprecise scoring, causing ambiguity. Dipolar electron paramagnetic resonance (EPR) provides spin-spin distances struggles to determine relative positions within complexes. We present a novel approach combining GPU-accelerated blind with EPR distance constraints enhance binding detection. Our...

Abstract Precise nanoscale distance measurements by pulsed electron paramagnetic resonance (EPR) spectroscopy play a crucial role in structural studies of biomolecules. The properties the spin labels used this approach determine sensitivity limits, attainable distances, and proximity to biological conditions. Herein, we propose validate use photoexcited fullerenes as for dipolar (PD) EPR measurements. Hyperpolarization narrower spectrum compared other triplets (e.g., porphyrins) boost...

Magnetic nanocomposites (MNCs) combine the features of magnetic nanoparticles and a second material, which provide distinct physical, chemical, biological properties. The core for nanocomposite synthesis is extensively used due to its high saturation magnetization, chemical stability, large surface area, easy functionalization. Moreover, (MNPs) have great potential resonance imaging (MRI), particle (MPI), hyperthermia, targeted drug gene delivery by an external field. Numerous composing...

Silica nanoparticles (SiNPs) are a promising material for nanomedicine technology. SiNPs considered powerful tool drug delivery, functional coatings and films, biomolecule separation due to their stability, biocompatibility, accessible surface modification. Herein, the synthesis of nylon 6 (SiNPs-Nylon) coated nanocomposites was proposed. Transmission electron microscopy (TEM) dynamic light scattering (DLS) were used morphology, size, stability analysis. Anticancer doxorubicin (DOX) loading...

Four albumin-nitroxide conjugates were prepared and tested as metal-free organic radical contrast agents (ORCAs) for magnetic resonance imaging (MRI). Each human serum albumin (HSA) carrier bears multiple nitroxides conjugated via homocysteine thiolactones. These molecular retain important physical biological properties of their HSA component, the resistance nitroxide groups to bioreduction was retained or enhanced. The relaxivities are similar these four much greater than those individual...

Establishing the Kirsten rat sarcoma (KRAS) mutational status is essential in terms of managing patients with various types cancer. Allele-specific real-time polymerase chain reaction (AS-PCR) a widely used method for somatic mutations detection. To improve limited sensitivity and specificity, several blocking methods have been introduced AS-PCR to block amplification wild-type templates. Herein, we novel modified oligonucleotide internucleotide phosphates reshaped...

Recently, Marina Bennati and coworkers (M. et al., Angew. Chem., Int. Ed., 2020, 59, 373-379., M. J. Magn. Reson., 2021, 333, 107091) proposed to use electron nuclear double resonance (ENDOR) spectroscopy in the W-band for a pair of labels, nitroxide 19F, measurements short (0.5-1.0 nm) distances biomolecules. In our paper, we investigated suitability high-field ENDOR pairs triarylmethyl fluorine labels using five newly synthesized model compounds. It is shown that application strong...

Homocysteine thiolactone is a five-membered cyclic thioester of amino acid homocysteine. It generated from homocysteine as result an error-editing reaction, principally, methionyl-tRNA synthetase. An elevated level associated with cardiovascular diseases, strokes, atherosclerosis, neurological abnormalities, etc., presumably because it reacts to the side chain protein lysine causing damage and autoimmune responses. not only important metabolite but also versatile building block for organic...

Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates oligonucleotides. Incorporating PG into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary detection limits in an allele-specific real-time polymerase chain reaction (AS-PCR). Herein, we used PG-modification improve specificity AS with unfavorable Pyr/Pur primer’s 3′-end mismatch template/primer complex. Two mutations PIK3CA gene (E542K, E545K)...

Abstract Precise nanoscale distance measurements by pulsed electron paramagnetic resonance (EPR) spectroscopy play a crucial role in structural studies of biomolecules. The properties the spin labels used this approach determine sensitivity limits, attainable distances, and proximity to biological conditions. Herein, we propose validate use photoexcited fullerenes as for dipolar (PD) EPR measurements. Hyperpolarization narrower spectrum compared other triplets (e.g., porphyrins) boost...

Magnetic Fe3O4 nanoparticles (MNPs) have great potential for nucleic acid separation, detection, and delivery. MNPs are considered a valuable tool in biomedicine due to their cost-effectiveness, stability, easy surface functionalization, the possibility of manipulations under magnetic field. Herein, synthesis nylon 6 nanocomposites (MNPs@Ny6) was investigated. Transmission electron microscopy (TEM) used morphology size analysis. A new method UV-induced immobilization oligonucleotides on...