A magnetic nanocomposite (MNC) is an integrated nanoplatform that combines a set of functions two types materials. successful combination can give rise to completely new material with unique physical, chemical, and biological properties. The core MNC provides the possibility resonance or particle imaging, field-influenced targeted delivery, hyperthermia, other outstanding applications. Recently, gained attention for external field-guided specific delivery cancer tissue. Further, drug loading...

Nanoplatforms used for the loading of anticancer drugs constitute a promising approach to cancer treatment and reducing side effects these drugs. Among cutting-edge systems in this area are magnetic nanocomposites (MNCs) nanocapsules (NCs). MNCs considered smart tool magnetic-field-guided targeted drug delivery, resonance imaging, hyperthermia therapy. Nanocapsules offer great potential due their ability control drug-loading capacity, release efficiency, stability, ease with which surfaces...

Silica nanoparticles (SiNPs) are a promising material for nanomedicine technology. SiNPs considered powerful tool drug delivery, functional coatings and films, biomolecule separation due to their stability, biocompatibility, accessible surface modification. Herein, the synthesis of nylon 6 (SiNPs-Nylon) coated nanocomposites was proposed. Transmission electron microscopy (TEM) dynamic light scattering (DLS) were used morphology, size, stability analysis. Anticancer doxorubicin (DOX) loading...

Cold atmospheric plasma (CAP) is an intensively-studied approach for the treatment of malignant neoplasms. Various active oxygen and nitrogen compounds are believed to be main cytotoxic effectors on biotargets; however, comprehensive mechanism CAP interaction with living cells tissues remains elusive. In this study, we experimentally determined optimal discharge regime (or semi-selective regime) direct jet cancer cells, under which lung adenocarcinoma A549, A427 NCI-H23 demonstrated...

Supramolecular constructions of amphiphilic dendritic molecules are promising vehicles for anti-cancer drug delivery due to the flexibility their architecture, high loading capacity and avoiding off-target effects a drug. Herein, we report new class species—triazine-carbosilane dendrons readily self-assembling into pH-sensitive dendrimersomes. The dendrimersomes efficiently encapsulate anticancer drugs doxorubicin methotrexate. Chemodrug-loaded have dose-related cytotoxic activity against...

Materials based on calcium carbonate (CaCO3) are widely used in biomedical research (e.g., as carriers of bioactive substances). The biocompatibility CaCO3 and dependence its stability pH make these materials promising transporters therapeutic agents to sites with low such a tumor tissue. In this work, we developed an approach the preparation nanoscale particles (CaNPs) up 200 nm size by coprecipitation analyzed interaction nanoparticles anticancer drug: DOXorubicin (DOX). We also showed...

Gold nanorods (GNRs) are considered one of the most promising forms nanoparticles for nanobiotechnology; however, problem their toxicity is currently not resolved. We synthesised GNRs, modified with linear polyethyleneimine (PEI-GNRs), and examined physicochemical some biological properties in comparison GNRs BSA spherical gold (sGNPs) same agents. The influence buffer, cell culture media, serum on hydrodynamic diameter zeta potential all GNPs was studied. Simultaneously, size, shape,...

Multifunctional gold nanoparticles (AuNPs) may serve as a scaffold to integrate diagnostic and therapeutic functions into one theranostic system, thereby simultaneously facilitating diagnosis therapy monitoring responses. Herein, albumin-AuNP agents have been obtained by conjugation of an anticancer nucleotide trifluorothymidine (TFT) or boron-neutron capture drug undecahydro-closo-dodecaborate (B12H12) bimodal human serum albumin (HSA) followed reacting the conjugates with AuNPs. In vitro...

Elaboration of non-viral vehicles for delivery therapeutic nucleic acids, in particular siRNA, into a cell is an actively growing field. Gold nanoparticles (AuNPs) occupy noticeable place these studies, and various nanoconstructions containing AuNPs are reported. We aimed our work to the rational design AuNPs-based siRNA vehicle with enhanced transfection efficiency. optimized obtaining non-covalent siRNAs-AuNPs cores: ionic strength, temperature reaction time were determined. Formation...

The composition of the protein corona covering any nanoparticle (NP) when it enters a biological fluid determines parameters NP’s interaction with body. To “control” these parameters, is important to know corona, determination which complex task associated two-layer organization (hard and soft coronas). In previous publication, we reported obtaining lipid-coated NPs full isolating them, proving presence on surface NPs. This work reports preparation, isolation, purification bearing hard...

Aeromonas popoffii is one of the environmental species. A number factors virulence have been described for this species and it has reported as a causative agent urinary tract infection. The first A. bacteriophage AerP_220 along with its host strain CEMTC 4062 were isolated from river water. phage podovirus morphotype, shows narrow range lytic against strain. genome comprises 45,207 bp does not contain genes responsible antibiotic resistance toxin production. Fifty-nine co-directional...

Hybrid magnetic molecularly imprinted polymers (MMIPs) have the advantages of technology material and nanoparticles. The properties MMIPs allow easy separation various pollutants analytes. A convenient simple approach has been developed for preparation based on polyamide (nylon-6) polymer matrix was formed during transition nylon-6 from a dissolved state to solid in presence template molecules Fe3O4 nanoparticles initial solution. Methylene blue (MB) used as model molecule. exhibited maximum...

Spontaneous sorption of proteins on the nanoparticles’ surface leads to fact that nanoparticles in biological media are always enveloped by a layer proteins—the protein corona. Corona affect properties and their behavior environment. In this regard, knowledge about composition corona is necessary element for development nanomedicine. Because have different efficacy, isolating particles with full characterizing challenging. study, we propose photo-activated cross-linker fixation. We believe...

Gold nanoparticles (AuNPs) are a platform for the creation of nanoconstructions that can have variety functions, including delivery therapeutic nucleic acids. We previously designed AuNP/small interfering RNA (siRNA) nanoconstruction consisting siRNA noncovalently bound on AuNP surface and showed this construction, when coated with lipid shell, was an efficient vehicle into cells. The goal present work to study possibility scaling up synthesis AuNP-siRNA its long-term storage without loss...

Use of multicellular spheroids in studies nanoparticles (NPs) has increased the last decade, however details NPs interaction with are poorly known. We synthesized AuNPs (12.0 ± 0.1 nm diameter, transmission electron microscopy (TEM data) and covered them bovine serum albumin (BSA) polyethyleneimine (PEI). Values hydrodynamic diameter were 17.4 0.4; 35.9 0.5 ±125.9 2.8 for AuNPs, AuBSA-NPs AuPEI-NPs, Z-potential (net charge) values -33.6 2.0; -35.7 1.8 39.9 1.3 mV, respectively. Spheroids...

The interaction of cold atmospheric plasma (CAP) with biotargets is accompanied by chemical reactions on their surfaces and insides, it has great potential as an anticancer approach. This study discovers the molecular mechanisms that may explain selective death tumor cells under CAP exposure. To reach this goal, transcriptional response to treatment was analyzed in A549 lung adenocarcinoma lung-fibroblast Wi-38 cells. We found induced common trend from cells—the p53 pathway, KRAS signaling,...

There is an urgent need to develop systems for nucleic acid delivery, especially the creation of effective therapeutics against various diseases. We have previously shown feasibility efficient delivery small interfering RNA by means gold nanoparticle-based multilayer nanoconstructs (MLNCs) suppressing reporter protein synthesis. The present work aimed at improving quality preparations desired MLNCs, and this purpose, optimal conditions their multistep fabrication were found. All steps...

Extracellular vesicles (EVs), carriers of molecular signals, are considered a critical link in maintaining homeostasis mammals. Currently, there is growing interest studying the role EVs, including exosomes (subpopulation EVs), animals other evolutionary levels, marine invertebrates. We have studied possibility obtaining appropriate preparations EVs from whole-body extract holothuria Eupentacta fraudatrix using standard combination centrifugation and ultracentrifugation. However, were...

Small interfering RNAs (siRNAs) are a powerful tool for specific suppression of protein synthesis in the cell, and this determines attractiveness siRNAs as drug. Low resistance siRNA to nucleases inability enter into target cells most crucial issues developing siRNA-based therapy. To face challenge, we designed multilayer nanoconstruct (MLNC) with AuNP core bearing chemically modified siRNAs. We applied chemical modifications 2'-OMe 2'-F substitutions well their combinations phosphoryl...

A protein corona (PC) is formed and maintained on the surface of any nanoparticle (NP) introduced into biological media. The full PC by a hard soft corona, latter determines nature interaction NPs with cells body's liquids. Nanomedicines are becoming increasingly important in modern health services, making information about composition PCs critically for "managing" behavior nano-objects body. Currently, only few studies report complete PC, since isolation preservation NP extremely difficult....

Globally, widespread tuberculosis is one of the acute problems healthcare. Drug-resistant forms require a personalized approach to treatment. Currently, rapid methods for detecting drug resistance Mycobacterium (MTB) some antituberculosis drugs are often used and involve optical, electrochemical, or PCR-based assays. Despite large number these assays, it necessary develop new tests (for drug-resistant MTB strains) that structurally simple do not specialized equipment. Colorimetric assays...

A protein corona is present on any nanoparticle (NP) entering biological fluids; however, the existence of a natural NPs has not been experimentally confirmed. We used our previously developed photomodification method to fix “biological nanoparticles” (bio-NPs) in fetal bovine serum and newborn serum; native sera served as control. To isolate photomodified bio-NPs, we ultracentrifugation (UC), sucrose gradient (12%, 30%, 50%), cushion (30%) methods. Isolated bio-NPs were visualized using...

A previously undescribed phenomenon of the inability cells to uptake gold nanoparticles (GNPs) was discovered when studying HepG2 under conditions 2D- and 3D- cultivation (monolayer spheroids). The same readily internalized GNPs functionalized with polyethyleneimine.