A5032323495- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Developmental Biology and Gene Regulation
- Infectious Diseases and Mycology
- Plant Pathogens and Fungal Diseases
- Molecular Biology Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Congenital heart defects research
- Pneumonia and Respiratory Infections
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Resistance in Staphylococcus
- Biosensors and Analytical Detection
- dental development and anomalies
- Central Venous Catheters and Hemodialysis
- Genomics and Chromatin Dynamics
- Congenital limb and hand anomalies
- Medical History and Research
- Heterotopic Ossification and Related Conditions
- Mobile Health and mHealth Applications
- Health Policy Implementation Science
- Biomedical and Chemical Research
- Genetics, Aging, and Longevity in Model Organisms
- Cell Image Analysis Techniques
Royal Zoological Society of Scotland
2023
Harvard University
2019-2022
Brigham and Women's Hospital
2019-2022
Center for Systems Biology
2019-2022
Michigan State University
2019
German Center for Infection Research
2018
Queen Mary University of London
2012-2016
Anglia Ruskin University
2015-2016
Tees, Esk and Wear Valleys NHS Foundation Trust
2014
Barts Health NHS Trust
2006-2010
Developmental enhancers integrate graded concentrations of transcription factors (TFs) to create sharp gene expression boundaries. Here we examine the hunchback P2 (HbP2) enhancer which drives a pattern in Drosophila blastoderm embryo response transcriptional activator Bicoid (Bcd). We systematically interrogate cis and trans that influence shape position driven by HbP2, find prevailing model, based on pairwise cooperative binding Bcd HbP2 is not adequate. demonstrate other proteins, such as...
Quantitative real-time PCR (qPCR) is increasingly used to detect Pneumocystis jirovecii for the diagnosis of pneumonia (PCP), but there are differences in nucleic acids targeted, DNA only versus whole acid (WNA), and also target genes amplification. Through Fungal Initiative, a working group International Society Human Animal Mycology, multicenter monocenter evaluation PCP qPCR assays was performed. For study, 16 reference laboratories from eight different countries, performing 20 analyzed...
The polymerase chain reaction (PCR) is widely used as a diagnostic tool in clinical laboratories and particularly effective for detecting identifying infectious agents which routine culture microscopy methods are inadequate. Invasive fungal disease (IFD) major cause of morbidity mortality immunosuppressed patients, optimal criteria contentious. Although PCR-based have long been the diagnosis invasive aspergillosis (IA), variable performance practice has limited their value. This shortcoming...
Invasive pulmonary aspergillosis (IPA) is a leading cause of morbidity and mortality in haematological malignancy patients hematopoietic stem cell transplant recipients(1). Detection IPA represents formidable diagnostic challenge and, the absence 'gold standard', relies on combination clinical data microbiology histopathology where feasible. Diagnosis must conform to European Organization for Research Treatment Cancer National Institute Allergy Infectious Diseases Mycology Study Group...
ABSTRACT Clinical experience with the impact of serum biomarkers for invasive fungal disease (IFD) varies markedly in hemato-oncology. Invasive pulmonary aspergillosis (IPA) is most common manifestation, so we evaluated bronchoalveolar lavage (BAL) fluid. An Aspergillus- specific lateral-flow device (LFD), quantitative real-time PCR (qPCR), and galactomannan (GM) test were used 32 BAL fluid samples from patients at risk IPA. Eight had proven IPA, 3 probable 6 possible 15 no IPA by European...
Diagnosis of invasive aspergillosis (IA) remains a challenge as the clinical manifestations are not specific, and histological diagnosis is often unfeasible. The 2002 European Organization for Research Treatment Cancer (EORTC) National Institute Allergy Infectious Diseases Mycoses Study Group (MSG) criteria classification cases into possible, probable or proven were revised in 2008. Our objective was to analyze impact these revisions on IA. A retrospective analysis 589 high risk...
A wide array of PCR tests has been developed to aid the diagnosis invasive aspergillosis (IA), providing technical diversity but limiting standardisation and acceptance. Methodological recommendations for testing blood samples using exist, based on achieving optimal assay sensitivity help exclude IA. Conversely, when more (BAL, biopsy, CSF) emphasis is placed confirming disease, so analytical specificity paramount. This multicenter study examined methods detecting IA by blind a panel DNA...
Background: Diagnosis of chronic pulmonary aspergillosis (CPA) is challenging. Symptoms are unspecific or missing, radiological findings variable and proof mycological evidence limited by the accuracy diagnostic tests. The goal this study was to investigate performance galactomannan (GM), newly formatted Aspergillus-specific lateral-flow-device test (LFD), a number cytokines in bronchoalveolar lavage fluid (BALF) samples obtained from patients with CPA, respiratory disorders without CPA...
Sense of community (SOC) is central to an individual's psychological wellbeing (Sarason, 1974). Eleven participants, mainly from the North West Western Australia, took part in semistructured interviews investigating Australian Aboriginal notions and SOC. Five key themes emerged data. These included: kinship structure, language groups, skin education, knowledge. It argued that groups described social structure whereas education knowledge maintenance a The impact this conclusion on theoretical...
Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. The need for accurate and rapid screening methods to detect MRSA carriers has been clearly established. performance of novel assay, BacLite Rapid (Acolyte Biomedica, UK) the detection (5 h) identification hospital associated ciprofloxacin resistant strains directly from nasal swab specimens was compared that obtained by culture on Mannitol salt agar containing Oxacillin (MSAO)...
Galactomannan (GM) is widely used for detection of invasive aspergillosis in high-risk haemato-oncology patients. Recent publications have reported a lack repeatability GM detection. The objective this retrospective study was to assess the levels during storage clinical samples. In screening strategy, positive sera were repeat tested as per manufacturer’s recommendations. Short-term (ST) samples at +4 °C while long-term (LT) −80 °C. Bronchoalveolar (BAL) fluid also repeating after ST and LT...
The proximity ligation assay (PLA) detects proteins via their interaction with pairs of probes, which are antibodies coupled to noncomplementary DNA oligonucleotides. binding both probes epitopes on the target protein brings oligonucleotides together, allowing them be bridged by a third oligonucleotide complementarity other two. This enables and detection resulting amplicon real-time quantitative PCR (qPCR), acts as surrogate marker for interest. Hence PLA has potential clinically relevant...
A paediatric oncology patient presented with central line sepsis caused by Vibrio harveyi, a gram negative bioluminescent marine bacterium known to be pathogenic fish and invertebrates, after swimming in the sea.
Aspergillosis is the primary fungal disease affecting captive penguins globally. Its diagnosis remains challenging, and currently no tests are both sensitive specific for detection of early infection. The present study evaluated a recently developed Aspergillus lateral-flow device (AspLFD) spp. antigen in plasma glottis mucus from penguins. In pilot retrospective study, banked frozen samples were reviewed: 11 gentoo (Pygoscelis papua papua) 4 king (Aptenodytes patagonicus) fulfilled...