To improve anti-inflammatory activity while reducing drug doses, we developed a nanoformulation carrying dexamethasone and butyrate.Dexamethasone cholesteryl butyrate-solid lipid nanoparticles (DxCb-SLN) were obtained with the warm microemulsion method. The of this novel has been investigated in vitro (cell adhesion to human vascular endothelial cells pro-inflammatory cytokine release by lipopolysaccharide-induced polymorphonuclear cells) vivo (disease index plasma concentrations dextran...

A specific method for the simultaneous determination of S-(+)Ibuprofen and R-(−)Ibuprofen enantiomers in human plasma is described. Adopting a high-performance liquid chromatographic (HPLC) system with spectrofluorometer detector, compounds were extracted from alcohol medium separated on C18 column, using solution acetonitrile–water–acetic acid–triethylamine as mobile phase. The limit quantitation was 0.1 µg/mL both compounds. validated by intra-day assays at three concentration levels used...

Sonodynamic therapy (SDT) exploits the energy generated by ultrasound (US) to activate sound-sensitive drugs (sonosensitizers), leading generation of reactive oxygen species (ROS) and cancer cell death. Two-dimensional (2D) three-dimensional (3D) cultures human pancreatic BxPC-3 cells were chosen as models with which investigate therapeutic effects US-activated sonosensitizer IR-780 is still one most lethal types cancer. The SDT, including ROS production, death immunogenic (ICD), extensively...

This study aims to evaluate the bioeffects of glutathione-responsive β-cyclodextrin-based nanosponges (GSH-NSs) on two- (2D) and three-dimensional (3D) cell cultures. The two types GSH-NS formulations, with low (GSH-NS B) high D) disulfide-bond content, were evaluated 2D colorectal (HCT116 HT-29) prostatic (DU-145 PC3) cancer In particular, cellular uptake was evaluated, as their effects growth, mitochondrial activity, membrane integrity, cycle distribution, mRNA expression, reactive oxygen...

The pharmacokinetics of ibuprofen enantiomers were investigated in a crossover study which seven healthy male volunteers received single oral doses 800 mg racemic as soluble granular formulation (sachet) containing L-arginine (designated trade name: Spedifen®), 400 (-)R-ibuprofen arginine or (+)S-ibuprofen arginine. Plasma levels both monitored up to 480 minutes after drug intake using an enantioselective analytical method (HPLC with ultraviolet detection) quantitation limit 0.25 mg/l....

Abstract 5‐Fluorouracil (5‐Fu) is a commonly used anticancer agent for treatment of solid tumours. Certain studies have reported conflicting results between individual plasma concentration levels and toxicity or therapeutic effects. For this reasons some authors proposed to evaluate the 5‐Fu metabolites 5‐fluorouridine, 5‐fluoro‐2′‐deoxyuridine 5‐fluoro‐5,6‐dihydro‐uracil. The aim present work develop validate new HPLC method simultaneously determining 5‐fluorouracil its three metabolites,...

Aims Platinum chemotherapy has been shown to have potent antineoplastic activity against various tumours, especially testicular, bladder, ovarian, head and neck cancers. This is accompanied by side‐effects of nephrotoxicity cumulative myelosuppression, the latter frequently presenting as severe anaemia. Cisplatin carboplatin might lower erythropoietin (Epo) secretion and, this mechanism, contribute anaemia that follows therapy with chemotherapeutic agent. The aim present work study plasma...

Pharmacokinetics (PK) studies the absorption and elimination of drugs in human body. Most PK models are parametric. Once it is identified, a model can estimate e.g. plasma concentrations over time for given individual. Population aims at making sensible predictions even when measurements so scarce to prevent identification. In fact those integrate past experimental evidence concerning some reference population. Again, integration typically relies on (meta)-parametric distribution individual...

Every drug whose copyright coverage has expired can be manufactured by a pharmaceutical company other than the one that developed it, giving rise to market of so-called generic drugs. It distinguished among two types drugs: branded and unbranded, according choice manufacturer assign it fantasy name or sell with active principle, followed company’s name. In order accepted for marketing, every new pass registration procedures Italian Ministry Health, which are simplified generics, as it’s...

Sonodynamic therapy (SDT) exploits the energy generated by ultrasound (US) to activate sound-sensitive drugs (sonosensitizers), leading generation of reactive oxygen species (ROS) and cancer cell death. Since pancreatic is still one most lethal types cancer, therapeutic effects US-activated sonosensitizer IR-780, have been investigated on two-dimensional (2D) three-dimensional (3D) cultures human BxPC-3 cells. The SDT were extensively including ROS production, death immunogenic (ICD). When...

Fluoxetine has been the first antidepressive agent of class selective serotonine reuptake inhibitors (SSRI) to be introduced in therapy, back 1987. The availability SSRIs meant a great advance treatment major depression and other neuro-psychiatric pathologies. In this paper we review scientific clinical research on fluoxetine that published last six years, as prior work extensively examined conducted tenth anniversary its introduction market. focuses cost minimization strategies can...

The study focuses on the microbiological and economic aspects of treatment hospital infections. following topics are presented discussed: I) principal features infections acquired during stays; II) data use fluoroquinolones in patients; III) cost-analysis infection treatment; IV) impact different antibiotics selection resistant strains Pseudomonas aeruginosa; V) review pharmacoeconomic antibacterial regimens.

Osteoporosis is a disorder of bone re-modeling, characterized by loss its micro-architecture and decreased density, which lead to fragility high risk fracture following moderate traumata. Osteoporosisrelated pathological fractures occur more frequently in post-menopausal women, the WHO estimates that osteoporotic women approximately 40% during lifetime. The prevention these considered one primary objectives for control economical health burden modern societies. first part this paper briefly...