A5082487200- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Pneumocystis jirovecii pneumonia detection and treatment
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Bacterial Infections and Vaccines
- Immune Cell Function and Interaction
- Chemical Synthesis and Analysis
- Proteoglycans and glycosaminoglycans research
- Computational Drug Discovery Methods
- Diabetes Management and Research
- Tuberculosis Research and Epidemiology
- ATP Synthase and ATPases Research
- Diabetes and associated disorders
- Mycobacterium research and diagnosis
University of Toledo
2016-2023
Memorial Sloan Kettering Cancer Center
2020
Utilizing natural antibodies to augment vaccine immunogenicity is a promising approach toward cancer immunotherapy. Anti-rhamnose (anti-Rha) are some of the most common anti-carbohydrate present in human serum. Therefore, rhamnose can be utilized as targeting moiety for rhamnose-containing prepare an effective formulation. It was shown previously that anti-Rha antibody generated mice binds effectively with Rha-conjugated and picked up by antigen presenting cells (APCs) through stimulatory Fc...
Pseudomonas aeruginosa (PA) is a Gram-negative pathogen that the World Health Organization has ranked as priority 1 (critical) threat. One potential prophylactic approach to preventing or reducing incidence of PA would be development long sought-after vaccine. Both antibody and CD4+ T-cell responses have been noted playing key roles in protection against infection. In these studies, we designed prototype vaccine consisting several known linear B-cell epitopes derived from an outer membrane...
Targeted delivery of antigens to antigen-presenting cells (APCs) by utilizing natural anticarbohydrate antibodies is a promising approach for selective uptake and enhanced antigen presentation. Previously, we reported that in the presence antibody, anti-rhamnose antibody (anti-Rha), bacterial sugar rhamnose conjugated with liposomal cancer MUC1-Tn enhances presentation APCs such as dendritic targeting Fc gamma receptors. The idea was utilize human anti-Rha present serum targeted...
<italic>N</italic>-Aryl urea derivatives were synthesized and some showed activity against mycobacterial hydrolases while others antimicrobial species.
Type 1 diabetes (T1D) is an autoimmune disorder characterized by cell mediated destruction of pancreatic beta cells. Pancreatic cells are the only source insulin in body. T1D patients then have to depend on injections for their lifetime. Insulin injection can modulate blood sugar levels, but has little effect process. Altered peptide ligands (APL) derived from known autoantigens able induce tolerance autoreactive animal models, currently unable elicit this protection humans. There a need...
The synthesis of a trisaccharide (common to glycoform I and II) tetrasaccharide I) from the outer core domain Pseudomonas aeruginosa lipopolysaccharide (LPS) using novel hydroquinone-based reducing-end capping group is reported. This multifunctional was utilized as purification handle stable toward many common transformations in oligosaccharide synthesis. access outer-core LPS antigens with TBDPS-protected hydroquinone (TPH) at reducing end will be useful for glycan array therapeutic glycoconjugate
A rhamnose targeting strategy for generating effective anticancer vaccines was successful in our previous studies. We showed that by utilizing natural anti-rhamnose antibodies, a rhamnose-containing vaccine can be targeted to antigen-presenting cells, such as dendritic cells. In this case, (Rha) linked directly the liposomes bearing antigen. However, current approach, we conjugated multivalent Tri-Rha ligand with antigen itself, making it single component construct, unlike two-component...
Oligosaccharide synthesis on organic solvent soluble, high molecular weight poly(2-hydroxyethylmethylacrylate) (pHEMA) is described. The pHEMA-bound oligosaccharide could be recovered after each reaction in 90-95% yield using a precipitation method. methodology was used to synthesize model tri-galactoside 48% overall and trisaccharide from the outer core domain of Pseudomonas aeruginosa lipopolysacchride (LPS) 39% yield. use photo-cleavable linker also demonstrated produce reducing-end...
Abstract Since anti rhamnose antibody is the most common natural anti-carbohydrate present in human serum, using as a targeting moiety for containing vaccine can be good approach effective formulation. Binding of rhamnose-containing with anti-rhamnose has been proven capturing and processing antigen by presenting cells (APC) via stimulatory Fc receptors. This was shown antibodies generated mice. We wished to determine if isolated from serum work similar manner. To isolate antibodies, we...
Abstract In order to obtain an effective cellular and humoral immune response, a vaccine construct has be captured, processed presented by antigen presenting cells (APCs) through their major histocompatibility complex (MHC) molecules. One way enhance presentation is antibody-dependent uptake mechanism targeting FcγR receptors of APCs that can bind with the Fc portion complexed antibody. Anti-Rhamnose (Anti-Rha) antibody among most common natural anti-carbohydrate antibodies present in human...