A5035383342- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Infections and bacterial resistance
- Antimicrobial Resistance in Staphylococcus
- COVID-19 diagnosis using AI
- Respiratory viral infections research
- Biosensors and Analytical Detection
- Mycobacterium research and diagnosis
- Mitochondrial Function and Pathology
- Plant Pathogenic Bacteria Studies
- Viral gastroenteritis research and epidemiology
- Infective Endocarditis Diagnosis and Management
- Pneumonia and Respiratory Infections
- COVID-19 Clinical Research Studies
- Metabolism and Genetic Disorders
- Cytomegalovirus and herpesvirus research
- Radiomics and Machine Learning in Medical Imaging
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
- Infectious Diseases and Mycology
National Defense Medical Center
2015-2025
Tri-Service General Hospital
2015-2025
Georgetown University Medical Center
2003-2005
Georgetown University
2003-2005
Tao Yuan General Hospital
1997
Changhua Christian Hospital
1997
To describe the immunological responses and clinical outcome of coronavirus (SARS) infected healthcare workers (HCW) who had been administered with convalescent plasma as a treatment.Convalescent (500 mL) was obtained from each three SARS patients transfused into HCW. Donors were blood type O seronegative for hepatitis B C, HIV, syphilis human T-cell lymphotropic virus types I II (HTLV-I -II). Serum antibody (IgG) titre >640. Apharesis performed CS 3000 plus cell separator followed by...
ABSTRACT We have designed a universal PCR capable of amplifying portion the 16S rRNA gene eubacteria, including Staphylococcus aureus , epidermidis Streptococcus pyogenes agalactiae pneumoniae Enterococcus faecium faecalis Mycobacterium tuberculosis Legionella pneumophila Escherichia coli Klebsiella Serratia marcescens Enterobacter cloacae Pseudomonas aeruginosa Acinetobacter baumannii Proteus mirabilis Haemophilus influenzae and Neisseria meningitidis . The sizes amplified products from...
SARS-CoV-2 has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms upper respiratory tract infection. We developed and validated a rapid total nucleic acid extraction method based on real-time reverse transcription-polymerase chain reaction (RT-PCR) reliable, high-throughput simultaneous detection common cold...
COVID-19 has spread globally. SARS-CoV-2 variants of concern (VOCs) are leading the next waves pandemic.
Molecular diagnosis of mitochondrial DNA disorder is usually focused on point mutations and large deletions. In the absence detectable mtDNA mutations, abnormal amounts mtDNA, either depletion or elevation, can be indicative dysfunction. The amount (mtDNA), however, varies among individuals different ages tissues within same individual. To establish a range levels, we analyzed 300 muscle 200 blood specimens from patients suspected having by real-time quantitative polymerase chain reaction...
Over-expression of AdeABC efflux pump stimulated continuously by the mutated AdeRS two component system has been found to result in antimicrobial resistance, even tigecycline (TGC) multidrug-resistant Acinetobacter baumannii (MRAB). Although insertion sequence, ISAba1, contributes one mutations, detail mechanism remains unclear. In present study we collected 130 TGC-resistant isolates from 317 carbapenem resistant MRAB (MRAB-C) isolates, and 38 them were characterized with ISAba1 adeS gene....
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic. Diagnostic testing for SARS-CoV-2 continuously been challenged due to several variants with diverse spike (S) and nucleocapsid (N) protein mutations []. variant proliferation potentially affects N protein-targeted rapid antigen testing. In this study, reverse transcription PCR (RT-PCR) tests were performed simultaneously in patients suspected disease 2019 (COVID-19). Direct whole genome sequencing was...
Abstract Leber hereditary optic neuropathy (LHON)/pediatric onset dystonia is associated with a G to A transition at nucleotide position (np) 14459, within the mitochondrial DNA (mtDNA)‐encoded ND6 gene. This mutation has been reported in families presenting LHON alone, plus dystonia, or pediatric typical age of less than 5 years. The changes moderately conserved alanine valine amino acid residue 72, which most evolutionarily region protein. Pediatric disease basal ganglia dysfunction,...
Background Breast cancer is a leading global health concern, necessitating advancements in recurrence prediction and management. The development of an artificial intelligence (AI)–based clinical decision support system (AI-CDSS) using ChatGPT addresses this need with the aim enhancing both accuracy user accessibility. Objective This study aims to develop validate advanced machine learning model for web-based AI-CDSS application, leveraging question-and-answer guidance capabilities enhance...
Background Sepsis, a critical global health challenge, accounted for approximately 20% of worldwide deaths in 2017. Although the Sequential Organ Failure Assessment (SOFA) score standardizes diagnosis organ dysfunction, early sepsis detection remains challenging due to its insidious symptoms. Current diagnostic methods, including clinical assessments and laboratory tests, frequently lack speed specificity needed timely intervention, particularly vulnerable populations such as older adults,...
Abstract Background MDR Elizabethkingia anophelis strains are implicated in an increasing number of healthcare-associated infections worldwide, including a recent cluster E. the Midwestern USA associated with significant morbidity and mortality. However, there is minimal information on antimicrobial susceptibilities or their resistance to carbapenems fluoroquinolones. Objectives Our aim was examine genetic profiles clinical isolates from our hospital, characterize carbapenemase genes...
With the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) B.1.1.7 lineage in ongoing coronavirus disease 2019 (COVID-19) pandemic, Taiwan confronted a COVID-19 flare up May 2021. Large-scale, accurate, affordable and rapid diagnostic tests such as lateral flow assay can help to prevent community transmission, but their performance characteristics real-world conditions relevant subpopulations remain unclear.The Antigen Rapid Test Kit (Eternal Materials, New Taipei...
Accurate molecular diagnostic assays for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, are needed epidemiology studies and to support infection-control measures. We evaluated analytical sensitivity clinical performance three sample-to-answer molecular-diagnostics systems SARS-CoV-2 using 325 nasopharyngeal swab samples from symptomatic patients.The BioFire Respiratory Panel 2.1 (RP2.1), cobas Liat Influenza A/B, Cepheid Xpert Xpress...
Clinical characteristics of patients in the first community outbreak coronavirus disease 2019 (COVID-19) by severe acute respiratory syndrome 2 (SARS-CoV-2) variant B.1.1.7 Taiwan have not been characterized.SARS-CoV-2 positive specimens from inpatients between May 7 and June 15 2021were screen for SARS-CoV-2 lineage VirSNiP assay. were reviewed compared with those Feb 1 to April 30, 2020 Jan March 31, 2022.One hundred forty-one 15, 2021 infected included. The major presenting symptoms fever...
Ninety-three clinical isolates of vancomycin-resistant enterococci (VRE) collected from nine hospitals in Taiwan were examined for the presence vanA , vanB vanC1 or vanC2/vanC3 genes by a multiplex PCR. Forty-seven these VRE positive, 1 contained both and 40 harboured 2 3 identified to be . Twenty-four sensitive teicoplanin thus did not have typical VanA phenotype. Five with VanC phenotype None clinically isolated vancomycin-susceptible E. faecium faecalis Leuconostoc Pediococcus positive...
Non-tuberculous mycobacteria (NTM) are increasingly important opportunistic pathogens responsible for a variety of clinical diseases. The aim this study was to evaluate novel technique, real-time PCR coupled with high-resolution melting analysis (real-time PCR-HRMA), NTM identification. Two pairs unique primers targeted the 16S rRNA gene and 16S-23S internal transcribed spacer region were selected further evaluation. A total 149 mycobacterial isolates subjected using PCR-HRMA system....