A5060212855- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Silicone and Siloxane Chemistry
- Polymer composites and self-healing
- Monoclonal and Polyclonal Antibodies Research
- Mechanical and Optical Resonators
- Marine Biology and Environmental Chemistry
- Advanced Polymer Synthesis and Characterization
- Acoustic Wave Resonator Technologies
- 3D Printing in Biomedical Research
- Advanced MEMS and NEMS Technologies
- High voltage insulation and dielectric phenomena
- Advanced Battery Materials and Technologies
- biodegradable polymer synthesis and properties
- Polyoxometalates: Synthesis and Applications
- Luminescence and Fluorescent Materials
- Covalent Organic Framework Applications
- Fuel Cells and Related Materials
- Advancements in Battery Materials
- Synthesis and properties of polymers
- Innovative Microfluidic and Catalytic Techniques Innovation
- Parasitic Infections and Diagnostics
- Advanced Sensor and Energy Harvesting Materials
- ZnO doping and properties
- Microfluidic and Capillary Electrophoresis Applications
Merck & Co., Inc., Rahway, NJ, USA (United States)
2016-2024
Jilin University
2018-2024
Hefei National Center for Physical Sciences at Nanoscale
2020-2024
University of Science and Technology of China
2020-2024
East China University of Science and Technology
2020-2022
Shanghai University of Engineering Science
2021
Zhengzhou University
2020-2021
Bristol-Myers Squibb (United States)
2013-2020
Shanghai Jiao Tong University
2016-2020
China Electric Power Research Institute
2018
Process intensification in biomanufacturing has attracted a great deal of interest recent years. Manufacturing platform improvements leading to higher cell density and bioreactor productivity have been pursued. Here we evaluated variety intensified mammalian culture processes for producing monoclonal antibodies. Cell operational modes including fed-batch (normal seeding or high with N-1 perfusion), perfusion, concentrated (CFB) were assessed using the same media set Chinese Hamster Ovary...
Li chains with up-down pairs on top sites are popular in silicene.
Bioreactor scale‐up is a critical step in the production of therapeutic proteins such as monoclonal antibodies (MAbs). With criterion similar power input per volume or O 2 volumetric mass transfer coefficient ( ), adequate oxygen supply and cell growth can be largely achieved. However, CO stripping phase often inadequate. This could cascade down to increased base addition osmolality, well residual lactate increase compromised product quality. Here we describe practical approach bioreactor...
Recent advances in high-throughput (HTP) automated mini-bioreactor systems have significantly improved development timelines for early-stage biologic programs. Automated platforms such as the ambr® 250 demonstrated ability, using appropriate scale-down approaches, to provide reliable estimates of process performance and product quality from bench pilot scale, but data sets comparing large-scale commercial processes (>10,000 L) are limited. As moves toward late stages, specifically...
Lactate metabolism variations are frequently encountered in mammalian cell culture processes, especially during process scale‐up. In this study, we took a multipronged approach to investigate the impact of pH, pCO 2 , osmolality, base addition, and mixing conditions on observed lactate Chinese Hamster Ovary (CHO) fed‐batch at 2,000 L scale. Two cultivating methods, CO ‐controlled pH‐controlled, were used decouple individual synergistic effects from those factors. The osmolality...
Double-decker silsesquioxane (DDSQ) and hindered urea bonds were simultaneously introduced into the main chains of linear segmented polyurethane (PU) to synthesize organic–inorganic poly(urea urethanes) (PUUs). The PUUs microphase-separated, POSS cages aggregated microdomains with size 10–20 nm. had improved thermomechanical properties in comparison those control PU. Notably, strong physical cross-linking was formed PUUs, thus materials could display thermoset elastomers. attributable...
Abstract The intermediate voltage electron microscope‐tandem user facility in the Electron Microscopy Center at Argonne National Laboratory is described. primary purpose of this microscopy with situ ion irradiation controlled sample temperatures. To illustrate its capabilities and advantages a few results two outside projects are presented. motion dislocation loops formed during illustrated video data that reveals striking reduction Fe‐8%Cr over pure Fe. development extended defect structure...
The current method for detecting the waterborne parasite Giardia lamblia is tedious and requires a preconcentration step. We show first time piezoelectric-excited millimeter-sized cantilever (PEMC) biosensor immobilized with monoclonal antibody against G. that exhibits selective sensitive detection of cysts in several water matrixes (buffer, tap, river water) at limit 1−10 cysts/mL without PEMC sensor resonance-based device functions high-order mode near 1 MHz. antibody-immobilized was...
ABSTRACT In this work, we synthesized a novel organic–inorganic semitelechelic polymer from polyhedral oligomeric silsesquioxane (POSS) and poly(acrylate amide) (PAA) via reversible addition‐fragmentation chain transfer (RAFT) polymerization. The polymers have been characterized by means of nuclear magnetic resonance spectroscopy, thermal gravimetric analysis, dynamic mechanical analysis. It was found that capping POSS groups to the single ends PAA chains caused series significant changes in...
Process mass intensity (PMI) is a benchmarking metric to evaluate the efficiency of manufacturing process, which indicative environmental impact process. Although this commonly applied for small molecule processes, it less biologics. In study, an Excel based tool developed by ACS GCI Pharmaceutical Roundtable was used calculate PMI different processes monoclonal antibody (mAb). For upstream three versions were compared: fed‐batch, fed‐batch with N‐1 perfusion, and perfusion in N‐stage...
Nickel oxide (NiO) has a cubic rock salt crystal structure at room temperature. Raman scattering of the transverse optical (TO) and longitudinal (LO) phonon in NiO is inactive. Thus, it difficult to employ technique study lattice vibration dynamics spin–phonon coupling NiO. In this work, crystalline stoichiometry nanoparticles with different nanocrystalline sizes was tuned make selection rules dramatically relaxed. Well-defined peaks two zone-boundary folded modes TO(Δ) LO(Δ) were observed....
In this work, carbon dots-decorated hydroxyapatite nanowires-lanthanide metal-organic framework composites were designed and synthesized as ratiometric fluorescent probes for the detection of dopamine. The as-prepared HAPNWs-CDs-Tb/MOF characterized by TEM, FE-SEM, FT-IR spectral analysis, illustrating that comprised nanowires acted a carrier to form spinning structure lanthanide MOF was decorated with dots. luminescent green fluorescence Tb3+ at 543 nm blue CDs 426 signal response groups...
In this paper, uniform Co–B amorphous alloy nanospheres with an average particle size of 50 nm were synthesized by chemical reduction cobalt ion borohydride in aqueous solution containing Bu4PBr and KCl. Then, Pd was introduced into system galvanic replacement reaction (GRR) between Co Na2PdCl4. Pd/Co–B catalysts different content could be obtained via adjusting the amount Na2PdCl4 mixture. The crystal structure, morphology, surface electronic state as-prepared characterized XRD, TEM, XPS,...
pH control is critical in bioreactor operations, typically realized through a two-sided loop, where CO2 sparging and base addition are used bicarbonate-buffered media. Though common approach, could compromise culture performance due to the potential impact from excursions osmolality increase large-scale bioreactors. In this study, feasibility of utilizing sparge gas composition as part loop was assessed Chinese hamster ovary (CHO) fed-batch cultures. Fine evaluated multiple processes at...