A5060622075- Enzyme-mediated dye degradation
- Algal biology and biofuel production
- Biochemical and biochemical processes
- HER2/EGFR in Cancer Research
- Photosynthetic Processes and Mechanisms
- Microbial bioremediation and biosurfactants
- Lignin and Wood Chemistry
- Biodiesel Production and Applications
- Antioxidant Activity and Oxidative Stress
- Quinazolinone synthesis and applications
- Cancer Treatment and Pharmacology
- Lung Cancer Treatments and Mutations
- Electrochemical sensors and biosensors
- Marine Bivalve and Aquaculture Studies
- Cancer-related Molecular Pathways
- Biocrusts and Microbial Ecology
- Metal-Catalyzed Oxygenation Mechanisms
- Food Industry and Aquatic Biology
- Monoclonal and Polyclonal Antibodies Research
- Marine and coastal plant biology
- Plant and fungal interactions
- Pesticide and Herbicide Environmental Studies
- Ruminant Nutrition and Digestive Physiology
- Cancer Mechanisms and Therapy
- Turfgrass Adaptation and Management
University of Greenwich
2011-2023
Centre for Cancer Biology
2023
Natural Resources Institute
2021-2022
Pfizer (United States)
2005-2016
California Maritime Academy
2007-2015
Cancer Society of New Zealand
2006
University of Auckland
2006
Imperial College London
1985-2003
Monsanto (United States)
1996
Texas A&M University
1995
Abstract PD 0332991 is a highly specific inhibitor of cyclin-dependent kinase 4 (Cdk4) (IC50, 0.011 μmol/L) and Cdk6 0.016 μmol/L), having no activity against panel 36 additional protein kinases. It potent antiproliferative agent retinoblastoma (Rb)-positive tumor cells in vitro, inducing an exclusive G1 arrest, with concomitant reduction phospho-Ser780/Ser795 on the Rb protein. Oral administration to mice bearing Colo-205 human colon carcinoma produces marked regression. Therapeutic doses...
A pharmacological approach to inhibition of cyclin-dependent kinases 4 and 6 (Cdk4/6) using highly selective small molecule inhibitors has the potential provide novel cancer therapies for clinical use. Achieving high levels selectivity Cdk4/6, versus other ATP-dependent kinases, presents a significant challenge. The pyrido[2,3-d]pyrimidin-7-one template provides an effective platform broad cross-section including Cdks. It is now demonstrated that modification pyrido[2,3-d]pyrimidin-7-ones...
In recent years there have been massive inundations of pelagic Sargassum, known as golden tides, on the beaches Caribbean, Gulf Mexico, and West Africa, causing considerable damage to local economy environment. Commercial exploration this biomass for food, fuel, pharmaceutical products could fund clean-up offset economic impact these tides. This paper reviews potential uses obstacles exploitation Sargassum. Although Sargassum has a source biochemicals, feed, fertiliser, variable undefined...
The oxidative capacity of the ligninase from Phanerochaete chrysosporium toward monomethoxylated and dimethoxylated aromatic compounds was investigated. Phenylacetic acid derivatives were shown to be decarboxylated by ligninase, via C‐C bond cleavage. Dimethoxylated substrates much more readily oxidised than derivatives, but oxidation latter could stimulated catalytic amounts 1,4‐dimethoxybenzene or veratryl alcohol. A mechanism based upon ability radical cations function as one‐electron...
Abstract Signaling through the erbB receptor family of tyrosine kinases contributes to proliferation, differentiation, migration, and survival a variety cell types. Abnormalities in members this have been shown play role oncogenesis, thus making them attractive targets for anticancer treatments. PF-00299804 is second-generation irreversible pan-erbB kinase inhibitor currently phase I clinical trials. believed irreversibly inhibit activity binding at ATP site covalent modification...
The recent discovery of an extracellular enzyme from Phanerochaete chrysosporium capable degrading lignin model compounds has countered the view that reactive diffusible oxygen species are responsible for biodegradation. In this paper we propose a mechanism which accounts both results obtained in enzymatic degradation studies and involving active species. quintessence is initial one‐electron oxidation or specific subunit followed by subsequent breakdown reactions via radical cation...
The green microalga Dunaliella salina survives in a wide range of salinities via mechanisms involving glycerol synthesis and degradation is exploited for large amounts nutraceutical carotenoids produced under stressed conditions. In this study, D. CCAP 19/30 was cultured varying photoperiods light intensities to study the relationship with different growth measurement parameters, cellular contents glycerol, starch carotenoids, photosynthesis respiration. Results show regulated cell volume...
Valorisation of the efficacy 9-cis beta-carotene in treating atherosclerosis, psoriasis, and inhibiting atherogenesis retinitis pigmentosa is becoming increasingly urgent, but supplies are scarce this compound difficult to synthesise chemically, unlike much more common all-trans form. Innovative products, processes services an algal biorefinery that rely on renewable biological resources instead fossil fuel alternatives offer potential lower energy costs traditional chemical reduce carbon...
The halotolerant photoautotrophic marine microalga Dunaliella salina is one of the richest sources natural carotenoids. Here we investigated effects high intensity blue, red and white light from emitting diodes (LED) on production carotenoids by strains D. under nutrient sufficiency strict temperature control favouring growth. Growth in was associated with carotenoid accumulation a rate oxygen uptake. On transfer to blue light, massive drop content recorded along very rates photo-oxidation....
The halotolerant microalga Dunaliella salina has been widely studied for natural β-carotene production. This work shows biochemical characterization of three newly isolated Dunaliellasalina strains, DF15, DF17, and DF40, compared with D. CCAP 19/30 UTEX 2538 (also known as bardawil). Although all new strains have genetically characterized their ability to accumulate carotenoids capacity photoprotection against high light stress are different. DF15 reveal great potential producing a large...
The identification of 8-ethyl-2-phenylamino-8H-pyrido[2, 3-d]pyrimidin-7-one (1) as an inhibitor Cdk4 led to the initiation a program evaluate related pyrido[2, 3-d]pyrimidin-7-ones for inhibition cyclin-dependent kinases (Cdks). Analysis more than 60 analogues has identified some clear SAR trends that may be exploited in design potent Cdk inhibitors. most inhibitors reported this study inhibit with IC(50) = 0.004 microM ([ATP] 25 microM). X-ray crystallographic analysis representative...
Inhibition of the cell cycle kinase, cyclin-dependent kinase-4 (Cdk4), is expected to provide an effective method for treatment proliferative diseases such as cancer. The pyrido[2,3-d]pyrimidin-7-one template has been identified previously a privileged structure inhibition ATP-dependent kinases, and good potency against Cdks reported representative examples. Obtaining selectivity individual Cdk enzymes, particularly Cdk4, challenging. Here, we report that introduction methyl substituent at...
Lignin degradation Single‐electron transfer Cα‐Cβ bond cleavage Phanerochaete chrysosporium Peroxidase compound I
An extracellular peroxidase capable of degrading a lignin-model compound has been isolated from cultures Coriolus versicolor, white-rot basidiomycete. The enzyme was produced in non-oxygenated stationary under conditions carbon limitation with the addition veratryl alcohol as an inducer. separated and shown to have oxidase activity, be oxidation wide range aromatic substrates presence H2O2. Its spectral characteristics showed it haemcontaining protein, sodium dodecyl sulphate-polyacrylamide...
Five isozymes of lignin peroxidase from Phanerochaete chrysosporium were purified and their physical, molecular kinetic properties determined. The differ each other in terms isoelectric point, mass, sugar content, spectral characteristics, substrate specificity stability. N‐terminal sequence amino acids was different for isozyme suggesting they are gene products. with the highest carbohydrate level most sensitive to changes environmental factors. behaviour varied clearly when tert ‐butyl...
Structure–activity relationships for inhibition of erbB1, erbB2, and erbB4 were determined a series quinazoline- pyrido[3,4-d]pyrimidine-based analogues the irreversible pan-erbB inhibitor, canertinib. Cyclic amine bearing crotonamides to provide rapid cellular erbB1 autophosphorylation good metabolic stability in liver microsome hepatocyte assays. The influence 4-anilino substitution on inhibitory potency was investigated. Several anilines identified as providing potent, reversible...
Dunaliella salina is a halotolerant, photoautotrophic marine microalga and one of the richest sources natural carotenoids but also shows potential as novel food source with high protein quality. This study sought to optimise production biomass, amino acids from D. salina, alongside using two-stage cultivation approach based on use light different intensities quality, i.e., white, red blue LED light. In stage 1, four white were tested. 2, same either or LEDs applied once exponential growth...