A5091565086- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Immune cells in cancer
- Chronic Lymphocytic Leukemia Research
- Lymphatic System and Diseases
- Cancer Research and Treatments
- Immune Response and Inflammation
- S100 Proteins and Annexins
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Vitamin C and Antioxidants Research
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Dermatologic Treatments and Research
- Signaling Pathways in Disease
- Yersinia bacterium, plague, ectoparasites research
- CAR-T cell therapy research
- Cancer, Stress, Anesthesia, and Immune Response
- Vascular Malformations and Hemangiomas
- Peptidase Inhibition and Analysis
- Ocular Oncology and Treatments
- Redox biology and oxidative stress
Medical University of Warsaw
2015-2024
Aminolevulinic acid (ALA) is an endogenous metabolite normally formed in the mitochondria from succinyl-CoA and glycine. Conjugation of eight ALA molecules yields protoporphyrin IX (PpIX) finally leads to formation heme. Conversion PpIX its downstream substrates requires activity a rate-limiting enzyme ferrochelatase. When administered externally abundantly produced cannot be quickly converted final product - heme by ferrochelatase therefore accumulates within cells. Since potent...
Abstract Studies on neutrophil extracellular traps ( NET s) are challenging as neutrophils live shortly and easily become activated. Thus, availability of a cell line model closely resembling the functions peripheral blood would be advantageous. Our purpose was to find compound that most effectively differentiates human promyelocytic leukemia (HL‐60) cells toward granulocyte‐like able release s. HL ‐60 were differentiated with all‐trans retinoic acid ATRA ), dimethyl sulfoxide DMSO ) or...
Despite great interest, the mechanism of neutrophil extracellular traps (NETs) release is not fully understood and some aspects this process, e.g. role reactive nitrogen species (RNS), still remain unclear. Therefore, our aim was to investigate mechanisms underlying RNS-induced formation NETs contribution RNS triggered by various physiological synthetic stimuli. The involvement in studied primary human neutrophils differentiated promyelocytic leukemia cells (HL-60 cells). (peroxynitrite...
Abstract Glucose-regulated protein 78 (GRP78) is an endoplasmic reticulum (ER)-resident chaperone and a major regulator of the unfolded response (UPR). Accumulating evidence indicate that GRP78 overexpressed in many cancer cell lines, contributes to invasion metastasis human tumors. Besides, upregulation detected different ER stress-inducing anticancer therapies, including photodynamic therapy (PDT). This study demonstrates mRNA levels are elevated PDT various lines. Stable overexpression...
Zinc plays an important physiological role in the entire body, especially immune system. It is one of most abundant microelements our organism and essential component enzymes antibacterial proteins. levels were reported to be correlated with intensity innate immunity responses, those triggered by neutrophils. However, as results are fragmentary, phenomenon still not fully understood requires further research. In this study, we aimed perform a comprehensive assessment study impact zinc on...
Lymphatic vessels transport fluid, antigens, and immune cells to the lymph nodes orchestrate adaptive immunity maintain peripheral tolerance. Lymphangiogenesis has been associated with inflammation, cancer metastasis, autoimmunity, tolerance transplant rejection, thus, targeted lymphatic ablation is a potential therapeutic strategy for treating or preventing such events. Here we define conditions that lead specific local closure of vasculature using photodynamic therapy (PDT)....
The release of neutrophil extracellular traps (NETs) can be either beneficial or detrimental for the host, thus it is necessary to maintain a balance between formation and clearance NETs. Multiple physiological factors eliciting NET have been identified, yet studies on natural signals limiting scarce. Accordingly, our aim was analyze whether cytokines immune cells inhibit formation. To that end, human granulocytes were incubated with interleukin (IL)-4, IL-10, transforming growth factor...
Abstract Background Impaired neutrophil activity is an important issue in chronic lymphocytic leukemia (CLL), as it contributes to a dysfunctional immune response leading life-threatening infections patients. Some features typical of CLL neutrophils, e.g., the B-cell-supportive secretion profile, have already been described. However, most these studies were performed on cells isolated from peripheral blood. It still unclear which molecular factors and cell types are involved shaping function...
Neutrophils are one of the first cells to arrive at site infection, where they apply several strategies kill pathogens: degranulation, respiratory burst, phagocytosis, and release neutrophil extracellular traps (NETs). Recent discoveries try connect NETs formation with autoimmune diseases, like systemic lupus erythematosus (SLE) or granulomatosis polyangiitis (GPA) place them among factors responsible for disease pathogenesis. The aim study was assess NETotic capabilities neutrophils...
It was previously reported that the activation of antitumor immune response by photodynamic therapy (PDT) is crucial for its therapeutic outcome. Excessive PDT-mediated inflammation accompanied immunosuppressive mechanisms protect tissues from destruction. Thus, final effect PDT strongly depends on balance between an adoptive arm and a range activated mechanisms. Here, with flow cytometry functional tests, we evaluate activity tumor-associated myeloid cells after PDT. We investigate...
Acute leukemias, the most common cancers in children, are characterized by excessive proliferation of malignant progenitor cells. As a consequence impaired blood cell production, leukemia patients susceptible to infectious complications—a major cause non-relapse mortality. Neutrophil extracellular traps (NETs) involved various pathologies, from autoimmunity cancer. Although aberrant NETs formation may be partially responsible for immune defects observed acute leukemia, still little is known...
Photodynamic therapy has been shown to induce strong immunity against tumor cells expressing exogenous tumor-associated antigens (TAAs), including P1A antigen. Cancer can evade the immune system by epigenetic silencing of TAAs, while DNA methyltransferase inhibitors, such as 5-aza-2'-deoxycytidine (5-aza-dC) restore expression silenced or downregulated TAA. Thus, remodeling with 5-aza-dC combined PDT elicit robust and durable antitumor immunity.
Neutrophils apply several antimicrobial strategies including degranulation, phagocytosis, the generation of reactive oxygen species (ROS) and release neutrophil extracellular traps (NETs) to fight pathogens. Iron is considered be an invaluable constituent host immune defense plays a dual role in immunity. It well-known component proteins necessary microelement for pathogen survival. The aim this study was broaden knowledge regarding impact iron on function neutrophils. from healthy blood...