A5011761766- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- Mycobacterium research and diagnosis
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Microbial Natural Products and Biosynthesis
- Tuberculosis Research and Epidemiology
- Antifungal resistance and susceptibility
- Nail Diseases and Treatments
- Genomics and Phylogenetic Studies
- Fungal Infections and Studies
Zhejiang University
2018-2025
Sir Run Run Shaw Hospital
2018-2025
This study is based on a CRKP screening program between patients and ward environments in an intensive care unit, describing the pKPC-2 ( bla KPC-2 -harboring plasmids) population structure evolutionary characteristics clinical settings. Long-read sequencing was performed genetically closely related strains, enabling high-resolution analysis of evolution pathway or within subgroups. We revealed extremely high rates multicopy antibiotic resistance genes (ARGs) settings its effect profile...
Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major nosocomial infectious pathogen with rapidly increasing prevalence. The genomic epidemiological characteristics of CRKP nationwide, especially the evolving trends within predominant clones, should be evaluated clearly.
Background: Methicillin-resistant Staphylococcus aureus (MRSA) remains a critical challenge in intensive care units (ICUs), yet the mechanisms driving its persistence and evolution this high-risk setting are not fully understood.
To determine the molecular characteristics and antimicrobial susceptibility of healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in Zhejiang Province.A total 391 HA-MRSA isolates were collected from 12 hospitals five cities Province, between January 2012 May 2013. Susceptibility to vancomycin, teicoplanin, linezolid, tigecycline, daptomycin was determined. Resistant screened for resistance mutations. Ten each hospital then chosen at random typing.The showed good all...
Currently, the mechanism of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) transmission is unclear; however, it must be considered in conjunction with asymptomatic S. strains colonization dynamics. This epidemiological study aimed to determine role household CA-MRSA China. Five patients culture-confirmed infection and five control were recruited from Sir Run Shaw Hospital Zhejiang, China, between December 2019 January 2020. The members patients, their pets,...
Fosfomycin has gained attention as a combination therapy for methicillin-resistant Staphylococcus aureus infections. Hence, the detection of novel fosfomycin-resistance mechanisms in S. is important. Here, minimal inhibitory concentrations (MICs) fosfomycin CC1 were determined. The pangenome analysis and comparative genomics used to analyse MRSA. gene function was confirmed by cloning into pTXΔ. A phylogenetic tree constructed determine clustering strains aureus. We identified gene,...
Abstract Staphylococcus capitis , which causes bloodstream infections in neonatal intensive care units, is a common cause of healthcare-associated infections. Thus, standardized high-resolution typing method to document the transmission and dissemination multidrug-resistant S. isolates required. We aimed establish core genome multilocus sequence (cgMLST) scheme surveil . The cgMLST was defined based on primary validation sets tested with outbreaks linezolid-resistant set. Phylogenetic...
Abstract Background The overuse of antibiotics in livestock is contributing to the burden antimicrobial resistance humans, representing a One Health challenge. Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has recently become growing concern, and ST9 major LA-MRSA lineage China emerged clinical settings. Methods Antimicrobial susceptibility testing was used evaluate tetracycline MRSA collections, gene cloning experiments were performed explore mechanisms....
ABSTRACT The aim of this study was to explore the mechanisms and molecular epidemiology carbapenem resistance in carbapenem-resistant Enterobacter cloacae complex (CRECC) over a decade tertiary hospital Zhejiang, China. From January 2011 December 2021, we collected total 931 (ECC) isolates from Antimicrobial susceptibility tests were performed. Whole-genome sequencing used analyze characteristics CRECC isolates. For strains, efflux inhibitor assay quantitative real-time PCR (qRT-PCR)...
Background Surgical sites infections (SSIs) caused by Methicillin-resistant Staphylococcus aureus (MRSA) constitute a major clinical problem. Understanding the transmission mode of MRSA is important for its prevention and control. Aim We investigated outbreak in trauma orthopedic hospital ward. Methods Clinical data were collected from patients ( n = 9) with infection ward January 1, 2015 to December 31, 2019. The wards 18), 48), medical staff 23), their households 5) screened MRSA. isolates...
Daptomycin (DAP) is effective against methicillin-resistant Staphylococcus aureus (MRSA). However, reduced susceptibility to DAP in MRSA may lead treatment failures. We aim determine the distribution of minimum inhibitory concentrations (MICs) and heteroresistance (hDAP) among lineages China. A total 472 clinical isolates collected from 2015 2017 China were examined for susceptibility. All (n = 472) found be susceptible, but 35.17% (166/472) them exhibited a high MIC (MIC >0.5 µg/mL). The...
Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a common nosocomial pathogen causing severe infectious diseases, and ST307 CRKP an emerging clone. In this study, we collected five isolates, evaluated their antimicrobial susceptibility using microbroth dilution, clonality population structure by PFGE, cgMLST, SNP-based phylogenetic analysis. Then, the genome characteristics, such as resistance genes plasmid profiles, were studied subsequent genomic The transfer ability was conjugation,...
Fosfomycin has regained attention for treating infections caused by methicillin-resistant Staphylococcus aureus and multidrug-resistant coagulase-negative staphylococci. In this research, our objective was to investigate the mechanisms underlying fosfomycin resistance in capitis. The minimum inhibitory concentrations (MICs) of were assessed 109 clinical S. capitis isolates agar dilution method. By cloning fos-like genes into shuttle vector, pTSSCm-Pcap, observing change MICs, gene function...
Cryptococcus spp. is a complex species that often causes cryptococcosis, which one of the most common opportunistic infections in adults living with HIV and has very high morbidity mortality rates. This study aimed to investigate antifungal susceptibility profiles epidemiological characteristics neoformans (CNSC) gattii (CGSC) Zhejiang Province, China. A total 177 CNSC 3 CGSC isolates were collected, was tested by FUNGUS verified an E-test. Moreover, multiple classification methods genomic...
Objective To determine the genetic structure of ermB -positive Tn1546-like mobile elements in methicillin-resistant Staphylococcus aureus (MRSA) from mainland China. Methods A total 271 erythromycin-resistant MRSA isolates were isolated Sir Run Shaw Hospital (SRRSH) 2013 to 2015. Whole-genome sequencing was performed for strains, and environment genes analyzed. Southern hybridization analysis transformation tests confirm location gene. Results 64 (64/271, 23.6%) with 62 strains (62/64,...