A5100627353- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Chromosomal and Genetic Variations
- Hepatitis B Virus Studies
- Genomics and Phylogenetic Studies
- MXene and MAX Phase Materials
- Animal Genetics and Reproduction
- COVID-19 Impact on Reproduction
- SARS-CoV-2 and COVID-19 Research
- Antibiotics Pharmacokinetics and Efficacy
- Supercapacitor Materials and Fabrication
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Plant Pathogenic Bacteria Studies
- Hepatitis C virus research
- Streptococcal Infections and Treatments
- Immune responses and vaccinations
- Conducting polymers and applications
- Bacterial Genetics and Biotechnology
- Liver Disease Diagnosis and Treatment
- Epoxy Resin Curing Processes
Zhejiang University
2013-2025
Sir Run Run Shaw Hospital
2019-2025
Hangzhou Medical College
2024-2025
Zhejiang Provincial People's Hospital
2024-2025
Wuhan Institute of Technology
2023-2024
Xiamen University
2022-2023
Sichuan Agricultural University
2022
Nanjing Medical University
2022
Institute of Microbial Technology
2021
Hangzhou Normal University
2021
The imbalance in the allocation healthcare resources between urban and rural areas has become a main focus of recent medical reforms adopted China. However, systematic analysis identified wide differences areas, including expenditures number facilities, available beds, personnel. Therefore, aim this report was to identify ethical considerations current governmental policies rectify existing problems distribution resources. Our findings indicate that inequality does not adhere standards are...
The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants and "anatomical escape" characteristics threaten the effectiveness current coronavirus disease 2019 (COVID-19) vaccines. There is an urgent need to understand immunological mechanism broad-spectrum tract protection guide broader vaccines development. Here we investigate immune responses induced by NS1-deleted influenza virus vectored intranasal COVID-19 vaccine (dNS1-RBD) which provides against SARS-CoV-2...
As highly efficient and clean electrochemical energy storage devices, supercapacitors (SCs) have drawn widespread attention as promising alternatives to batteries in recent years. Among various electrode materials, iron oxide materials been widely studied negative SC due their broad working window potential, ideal theoretical specific capacitance, good redox activity, abundant availability, eco-friendliness. However, oxides still suffer from the problems of low stability poor conductivity....
Background: Methicillin-resistant Staphylococcus aureus (MRSA) remains a critical challenge in intensive care units (ICUs), yet the mechanisms driving its persistence and evolution this high-risk setting are not fully understood.
Methicillin resistant Staphylococcus aureus (MRSA) is one of the main causes hospital-acquired infections, but diagnosis MRSA pseudomembranous enteritis has faded in recent years. Here, we reported a case young male patient caused by ST5 MRSA. Clinical data were collected from medical records. Mass spectrometry identification, antimicrobial susceptibility testing, whole genome sequencing, as well resistance and virulence genes detection strain performed. A 27-year-old man with acute...
ABSTRACT Compared with tigecycline-resistant gram-negative bacteria, tigecycline adaptive laboratory evolution (ALE) has been preliminarily performed in Staphylococcus aureus . This study aims to develop higher-level S. mutants (TRSAms) and explore the mechanisms behind decreasing susceptibility tigecycline. In this study, strains were cultured serial-increasing concentrations of successfully obtained high-level TRSAms. Different phenotypic changes TRSAms assessed by growth rate measurement,...
ABSTRACT A novel major facilitator superfamily (MFS) efflux pump in Staphylococcus , designated Nms, was identified via topology prediction. The secondary structure indicated the presence of 12 transmembrane segments (TMSs) and characteristic motif MFS pumps. Experimental verification activity conducted using ethidium bromide accumulation assays biofilm formation assays. Antimicrobial susceptibility testing inhibition confirmed that Nms effectively effluxed various antimicrobial agents to...
ABSTRACT Vancomycin heteroresistance is prone to missed detection and poses a risk of clinical treatment failure. We encountered one Enterococcus faecium strain, SRR12, that carried complete vanM gene cluster but was determined as susceptible vancomycin using the broth microdilution method. However, distinct subcolonies appeared within clear zone inhibition in E-test assay, which, named SRR12-v1, showed high-level resistance vancomycin. SRR12 confirmed heteroresistant population analysis...
The effect of the vinyl content on microstructure, thermodynamics and dynamics properties graphene/SSBR composites was investigated. SSBR with highest has interfacial shear stress by pullout simulation.
Vancomycin-variable enterococci (VVE) are a potential risk factor for vancomycin resistance gene dissemination and clinical treatment failure. vanM has emerged as new prevalent determinant among in China. A total of 54 vancomycin-susceptible (VSE) isolates carrying incomplete clusters were isolated our previous study.To determine the vanM-carrying VSE to develop investigate mechanism alteration phenotype.Fifty-four vanM-positive strains induced vitro by culturing increasing concentrations...
Fosfomycin has gained attention as a combination therapy for methicillin-resistant Staphylococcus aureus infections. Hence, the detection of novel fosfomycin-resistance mechanisms in S. is important. Here, minimal inhibitory concentrations (MICs) fosfomycin CC1 were determined. The pangenome analysis and comparative genomics used to analyse MRSA. gene function was confirmed by cloning into pTXΔ. A phylogenetic tree constructed determine clustering strains aureus. We identified gene,...
Abstract Staphylococcus capitis , which causes bloodstream infections in neonatal intensive care units, is a common cause of healthcare-associated infections. Thus, standardized high-resolution typing method to document the transmission and dissemination multidrug-resistant S. isolates required. We aimed establish core genome multilocus sequence (cgMLST) scheme surveil . The cgMLST was defined based on primary validation sets tested with outbreaks linezolid-resistant set. Phylogenetic...
The aim of this study was to investigate the clinical and microbiological features community-onset CRE (CO-CRE) obtained from outpatients at a tertiary hospital in China.We isolated 64 strains divided them into three groups: 36 hospital-acquired (HA-CRE), 28 CO-CRE including 15 community-acquired (CA-CRE) 13 healthcare-associated (HCA-CRE). Clinical information collected. antibiotic susceptibilities were tested. Whole-genome sequencing (WGS) conducted, then drug resistance gene analysis...
Polyetherketoneketone (PEKK) is considered to be a potential substitute material for metal bone implants because of its advantageous biocompatibility, chemical stability, and mechanical properties, but clinical application has been severely restricted due PEKK's lack antibacterial ability biological activity. In this study, LL-37, natural human antimicrobial peptide, was successfully modified on the PEKK surface with polydopamine as intermediate layer released continuously more than 6 days....
Abstract Background The overuse of antibiotics in livestock is contributing to the burden antimicrobial resistance humans, representing a One Health challenge. Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has recently become growing concern, and ST9 major LA-MRSA lineage China emerged clinical settings. Methods Antimicrobial susceptibility testing was used evaluate tetracycline MRSA collections, gene cloning experiments were performed explore mechanisms....
Vancomycin-resistant enterococcus (VRE) and methicillin-resistant Staphylococcus aureus (MRSA) are significant healthcare-associated pathogens. We sought to identify factors that could be used predict which patients carry or infected with VRE MRSA on admission so we obtain cultures selectively from high-risk our burn-trauma unit. conducted a case-control study of admitted unit September 2000 March 2005 who were colonized either (cases) not one these organisms (controls). logistic regression...
The allotetraploid hybrid fish (4nAT) that was created in a previous study through an intergeneric cross between red crucian carp (Carassius auratus var., ♀) and common (Cyprinus carpio L., ♂) provided excellent platform to investigate the effect of hybridization polyploidization on evolution 5S rDNA. rDNAs paternal were made up coding sequence (CDS) non-transcribed spacer (NTS) unit, while maternal contained CDS NTS they also variable number interposed regions (IPRs). CDSs both parental...
Treatment failure of methicillin-resistant Staphylococcus aureus (MRSA) infections remains problematic in clinical practice because therapeutic options are limited. Penicillin plus potassium clavulanate combination (PENC) was shown to have potential for treating some MRSA infections. We investigated the susceptibility isolates and constructed a drug prediction model phenotype PENC. determined minimum inhibitory concentration PENC ( n =284) teaching hospital (SRRSH-MRSA). genotypes were...