A5075076262- Advanced Fluorescence Microscopy Techniques
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Viral Infectious Diseases and Gene Expression in Insects
- MicroRNA in disease regulation
- RNA modifications and cancer
Karolinska Institutet
2019-2020
Gaining insight into the uptake, trafficking and target engagement of drugs in cells can enhance understanding a drug's function efficiency. However, there are currently no reliable methods for studying untagged biomolecules macromolecular complexes intact human cells. Here we have studied an antisense oligonucleotide (ASO) drug HEK 293T HeLa by NMR spectroscopy. Using combination transfection, cryoprotection dynamic nuclear polarization (DNP), were able to detect directly frozen Activity...
Abstract Gaining insight into the uptake of drugs cells, trafficking and their target engagement enhances understanding drug’s function efficiency. Here we study an antisense oligonucleotide drug (ASO) delivered HEK293T HeLa by Nuclear Magnetic Resonance (NMR). Using a combination transfection, cryoprotection dynamic nuclear polarization (DNP), were able to detect directly in intact frozen cells. Activity was confirmed qRT-PCR, measuring downregulation its mSTAT3. Applying DNP NMR overcome...