A5028625177- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Protein Structure and Dynamics
- Virology and Viral Diseases
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
Karolinska Institutet
2018-2024
Technical University of Munich
2021
BioTalentum (Hungary)
2017
Interuniversity Consortium for Magnetic Resonance
2014
Aix-Marseille Université
2014
Architecture et Fonction des Macromolécules Biologiques
2014
Centre National de la Recherche Scientifique
2014
Abstract We provide an atomic‐resolution description based on NMR spectroscopy, of the intrinsically disordered C‐terminal domain Nipah virus nucleoprotein (N TAIL ), both in its isolated state and within nucleocapsid (NC). Within NC second half N retains conformational behavior similar to that , whereas first becomes much more rigid. In spite mostly nature chemical shifts relaxation measurements show a significant degree α‐helical sampling molecular recognition element (MoRE) involved...
Recent findings in genome-wide transcriptomics revealed that RNAs are involved almost every biological process, across all domains of life. The characterization native unknown function and structure is particularly challenging due to their typical low abundance the cell inherent sensitivity toward ubiquitous RNA degrading enzymes. Therefore, robust vitro synthesis extensive work-up methods often needed obtain samples amenable for biochemical, biophysical, structural studies. Here, we present...
ABSTRACT microRNAs (miRNAs) regulate target mRNA expression post-transcriptionally through their association with Argonaute 2 (AGO2) proteins. Predicting the efficiency of repression by miRNA has been limited our comprehension structure-function relationship within binding sites. Using a combination EMSA, luciferase reporter assays, and structural probing, we investigated interaction between human tumour suppressor miR-34a 12 targets. Comparison direct RNA:RNA interactions those functional...
There is an increasing demand for efficient and robust production of short RNA molecules in both pharmaceutics research. A standard method vitro transcription by T7 polymerase. This sequence-dependent on efficiency limited to products longer than ~12 nucleotides. Additionally, the native initiation sequence required achieve high yields, putting a strain variability. Deviations from this can lead side products, requiring laborious purification, further decreasing yield. We here present...
RNA regulation can be performed by a second targeting molecule, such as in the microRNA mechanism. Selective 2′-hydroxyl acylation analyzed primer extension (SHAPE) probes structure of molecules and resolve RNA:protein interactions, but RNA:RNA interactions have not yet been addressed with this technique. Here, we apply SHAPE to investigate RNA-mediated binding processes RNA:RNA-RBP complexes. We use R NA:RN A b inding S HAPE (RABS) microRNA-34a ( miR-34a ) its mRNA target, silent...
Abstract The understanding of the functional importance RNA has increased enormously in last decades. This required research on molecules themselves, with concomitant need for obtaining purified samples, such as structural studies by NMR or other methods. main method to create labeled and unlabeled RNA, T7 vitro transcription, suffers from sequence‐dependent yield often low homogeneity short constructs (<100 nt) requires laborious purification. Additionally, design structured fragments...