A5010324540- Chronic Lymphocytic Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- Multiple Myeloma Research and Treatments
- Lymphoma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- Immunodeficiency and Autoimmune Disorders
- Cancer Genomics and Diagnostics
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- PI3K/AKT/mTOR signaling in cancer
- Cancer therapeutics and mechanisms
- Ubiquitin and proteasome pathways
- Cancer Mechanisms and Therapy
- Viral-associated cancers and disorders
- Estrogen and related hormone effects
- Cancer Immunotherapy and Biomarkers
- Tryptophan and brain disorders
- CAR-T cell therapy research
- Cutaneous lymphoproliferative disorders research
- Protein Degradation and Inhibitors
- Cancer-related cognitive impairment studies
- Peripheral Neuropathies and Disorders
University of Southampton
2011-2023
University Hospital Southampton NHS Foundation Trust
2008-2017
Southampton General Hospital
1993-2016
Faculty (United Kingdom)
2013
Tenovus Cancer Care
1997-2008
University of Oslo
2007
Oslo University Hospital
2007
National Institute for Biological Standards and Control
2007
Centre Virchow-Villermé
2003
Centre Henri Becquerel
2003
Oesophageal adenocarcinoma (OAC) is increasingly common in the west, and survival remains poor at 10–15 % 5 years. Immune responses are implicated as a determining factor of tumour progression. The ability lymphocytes to recognise antigens provides mechanism for host immune attack against cancer providing potential treatment strategy. Tumour infiltrating (TILs: CD3+, CD4+, CD8+ FOXp3+) were assessed by immunohistochemistry using tissue microarrays contemporary homogeneous cohort OAC patients...
When cells transform, phenotypic and genetic profiles can be dramatically altered. Nevertheless, a recent report identifying IgG in breast cancer was unexpected, revealing differentiation features normally associated with B lymphocytes. To extend these findings, we focused on immunoglobulin variable (V) region gene analysis using well-defined cell lines expressing the epithelial marker, adhesion molecule (EpCAM). V(H) transcripts were identifiable by nested reverse transcription-PCR either...
Sixty-four strains of intestinal bacteria were cultured under anaerobic conditions in lactulose-containing media to assess their ability ferment lactulose. Some organisms unable metabolize the disaccharide, while others, e.g. clostridia and lactobacilli, metabolized lactulose extensively. Quantitative analyses fermentation products indicated that major non-gaseous metabolites acetic, lactic butyric acids. Hydrogen carbon dioxide only gases detected. Fermentation estimated for selected...
Background In multiple myeloma, expression of cancer testis antigens may provide prognostic markers and potential targets for immunotherapy. Expression at relapse has not yet been evaluated a large panel which can be classified by varying in normal tissue: restricted to testis, expressed brain but selectively testis.Design Methods Evaluation antigen was made newly diagnosed myeloma cases (HOVON-65/GMMG-HD4 trial; n=320) (APEX, SUMMIT, CREST trials; n=264). Presence using Affymetrix GeneChips...
// Alboukadel Kassambara 1,2 , Claire Gourzones-Dmitriev 2 Surinder Sahota 3 Thierry Rème Jérôme Moreaux 1 Hartmut Goldschmidt 4 Angelos Constantinou 5 Philippe Pasero Dirk Hose Bernard Klein 1,2,6 CHU Montpellier, Institute of Research in Biotherapy, F-34295 FRANCE; INSERM, U1040, F-34197 France; Cancer Sciences Unit, Faculty Medicine, University Southampton, UK Medizinische Klinik V, Universitaetsklinikum Heidelberg, Heidelberg D-69120 GERMANY; Human Genetics, CNRS-UPR1142, Montpellier...
We previously reported ongoing mutational and isotype switch events in the immunoglobulin (Ig) heavy chain (H) locus hairy cell leukemia. Those analyses raised questions on incidence type of selective influences occurring tumor B-cell receptor leukemia.To further investigate this issue, we examined full IGH kappa lambda light chains (IGkappa IGlambda) variable constant region transcripts expressed a large cohort patients with leukemia (n=88).Multiple IgH isotypes were 46/56 (82%) cases...
// Wafa Hassen 1,2 , Alboukadel Kassambara 1,3 Thierry Reme Surinder Sahota 4 Anja Seckinger 5,6 Laure Vincent 7 Guillaume Cartron Jérôme Moreaux 1,3,8 Dirk Hose and Bernard Klein 1 Institute of Human Genetics, CNRS-UPR1142, Montpellier, France 2 High Biotechnology Monastir, University Tunisia 3 CHU Laboratory for Monitoring Innovative Therapies, Department Biological Haematology, Cancer Sciences Unit, Faculty Medicine, Southampton, UK 5 Medizinische Klinik V,...
In understanding clonal evolution in cancer, whole genome sequencing (WGS) provides unparalleled data that can be leveraged to derive tumor phylogenies.Specifically, estimates of the cancer cell fraction (CCF), is cells bearing a set somatic mutations, highly informative by defining which mutations are and associated with most recent common ancestor (MRCA) all cells, occur subclonally as later events.By clustering observed based on their CCF subsequent phylogenetic tree inference, derived...
In classic Hairy cell leukaemia (HCLc), a single case has thus far been interrogated by whole exome sequencing (WES) in treatment naive patient, which BRAF V(600)E was identified as an acquired somatic mutation and confirmed occurring near-universally this form of disease conventional PCR-based cohort screens. It left open however the question whether other genome-wide mutations may also commonly occur at high frequency presentation HCLc disease. To address this, we have carried out WES 5...