A5020908496- Gastric Cancer Management and Outcomes
- Gastrointestinal disorders and treatments
- IgG4-Related and Inflammatory Diseases
- Genetic factors in colorectal cancer
- Colorectal Cancer Screening and Detection
- Neuroendocrine Tumor Research Advances
- Colorectal Cancer Surgical Treatments
- Pancreatic and Hepatic Oncology Research
- Colorectal and Anal Carcinomas
- Esophageal Cancer Research and Treatment
- Gastrointestinal Tumor Research and Treatment
- Pancreatitis Pathology and Treatment
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Breast Lesions and Carcinomas
- T-cell and B-cell Immunology
- Cancer and Skin Lesions
- Breast Cancer Treatment Studies
- Liver Disease Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Microscopic Colitis
- Cancer Immunotherapy and Biomarkers
- Radiomics and Machine Learning in Medical Imaging
- Inflammatory Bowel Disease
Southampton General Hospital
2015-2025
University Hospital Southampton NHS Foundation Trust
2016-2025
University of Southampton
2006-2020
University of Bristol
2020
Bristol Hospital
2020
University College London
2019
NIHR Imperial Biomedical Research Centre
2018
Hampton University
2005-2017
National Health Service
2014
Research Network (United States)
2012
Nonalcoholic fatty liver disease (NAFLD) describes an increasingly prevalent spectrum of disorders associated with obesity and metabolic syndrome. It is uncertain why steatosis occurs in some individuals, whereas nonalcoholic steatohepatitis (NASH) others. We have generated a novel mouse model to test our hypothesis: that maternal fat intake contributes the development NAFLD adult offspring. Female mice were fed either high-fat (HF) or control chow (C) diet before during gestation lactation....
There is no licensed treatment for non-alcoholic fatty liver disease (NAFLD), a condition that increases risk of chronic disease, type 2 diabetes and cardiovascular disease. We tested whether 15-18 months with docosahexaenoic acid (DHA) plus eicosapentaenoic (EPA) (Omacor/Lovaza) (4 g/day) decreased fat improved two histologically-validated fibrosis biomarker scores (primary outcomes). Patients NAFLD were randomised in double blind placebo-controlled trial [DHA+EPA(n=51), placebo(n=52)]....
OBJECTIVES: Elevated serum immunoglobulin G4 (IgG4) levels have been associated with autoimmune pancreatitis and IgG4-related disease (IgG4-RD) for over a decade. However, an elevated IgG4 is not specific the disease. There inconsistent reports of its use in diagnosis, as marker relapse, relationship to organ involvement retrospective cohorts. The aims this study were ascertain conditions that are investigate role prospective cohort patients IgG4-RD. METHODS: We evaluated measurements Oxford...
Abstract Background Intestinal macrophages are key immune cells in the maintenance of intestinal homeostasis and have a role pathogenesis inflammatory bowel disease (IBD). However, mechanisms by which exert pathological influence both ulcerative colitis (UC) Crohn (CD) not yet well understood. Methods We purified from gastrointestinal mucosal biopsies (patients with UC, patients CD, healthy donors) analyzed their transcriptome RNA sequencing bioinformatics, confirming results quantitative...
Mucosal changes in inflammatory bowel disease (IBD) are characterized by ulcerative lesions accompanied prominent cellular infiltrates the wall. Chemokines chemotactic cytokines that able to promote leukocyte migration areas of inflammation and also initiate cell activation events. They have recently been implicated pathophysiology many states. The aim this study was detail degree distribution specific chemokines, interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, -2, -3,...
Abstract Purpose: The purpose of this study was to determine the role functional interactions between pancreatic cancer cells and stellate (PSCs) in formation desmoplastic reaction (DR) characterize effect type I collagen (the predominant component DR) on cell phenotype. Experimental Design: PSCs were identified sections using immunohistochemistry, their anatomic relationship studied. Interactions among lines (MIA PaCa-2, Panc-1, AsPC-1), primary cultures human PSCs, investigated a series...
IgG subclass 4-related disease (IgG4-RD) is characterized by increased serum levels of IgG4 and infiltration biliary, pancreatic, other tissues IgG4-positive plasma cells. We assessed the prevalence allergy and/or atopy, serum, tissue IgE antibodies, blood eosinophils in patients with IgG4-RD. investigated association between diagnosis relapse this disease.We performed a prospective study 48 IgG4-RD, 42 an level inflammatory autoimmune conditions (disease control subjects), 51 healthy...
Oesophageal adenocarcinoma (OAC) is increasingly common in the west, and survival remains poor at 10–15 % 5 years. Immune responses are implicated as a determining factor of tumour progression. The ability lymphocytes to recognise antigens provides mechanism for host immune attack against cancer providing potential treatment strategy. Tumour infiltrating (TILs: CD3+, CD4+, CD8+ FOXp3+) were assessed by immunohistochemistry using tissue microarrays contemporary homogeneous cohort OAC patients...
Background: Achieving evidence-based practice change in surgery has always been challenging, with many aspects of common clinical evolving through lower-level studies that are susceptible to bias and confounding rather than high-quality evidence. This challenge is even more pronounced the setting low-volume, high-complexity surgical oncology. Additionally, when costs interventions or technologies high, designing developing such within financially constrained national healthcare systems...
Summary Single nucleotide polymorphisms (SNPs) in the promoter regions of cytokine genes are associated with differential levels expression. We hypothesized that these SNPs might influence breast tumour development and progression by affecting efficiency antitumour immune response and/or pathways angiogenesis. A total 144 female cancer patients 263 cancer‐free population controls were genotyped for interleukin (IL)‐1β−511 (T/C), IL‐6 −174 (G/C), necrosis factor (TNF)‐α−308 (A/G), IL‐10 −1082...
AIMS--To evaluate the effect of microwave irradiation on staining quality a range commonly used primary antibodies in archival, formalin fixed, paraffin wax embedded material, with emphasis that have previously worked successfully only frozen tissue. METHODS--Immunocytochemistry (streptavidin-biotin complex technique) was performed histological sections normal and pathological tissues, after varying treatment irradiation. The each antibody compared achieved without prior or enzyme...
<h3>Introduction</h3> The Li–Fraumeni Syndrome is caused by a germline <i>TP53</i> mutation and associated with high risk of breast cancer at young ages. Basal (triple negative) cancers are now well recognised to be typical sub-type developing in large proportion BRCA1 gene carriers. We considered whether similar narrow was found <h3>Objective</h3> A hypothesis generating study investigate there specific tumour characteristics mutations. <h3>Methods</h3> Pathological 12 arising nine patients...
Background and AimAlcohol-related liver disease (ALD) remains a leading cause of liver-related morbidity mortality. Age, fibrosis stage, MELD score continued alcohol consumption predict outcome in everyday clinical practice. In previous studies increased hepatocyte nuclear area expression p21, both markers senescence, were associated with stage poor non-alcohol-related fatty disease, while was related to dysfunction ALD cirrhosis. This study, therefore, investigated the pattern cell cycle...
Albumin, widely recognized as a highly sensitive and specific marker of hepatocellular carcinoma (HCC), is currently unavailable in the diagnostic laboratory because lack robust platform. In prior study we detected albumin mRNA majority intrahepatic cholangiocarcinomas using novel branched chain RNA situ hybridization (ISH) We now explore utility ISH differentiation HCCs, compare its sensitivity with Hep Par 1 Arginase-1. evaluated 93 HCCs mimics including neuroendocrine tumors...
Objectives Counting intraepithelial lymphocytes (IEL) is central to the histological diagnosis of coeliac disease (CD), but no definitive ‘normal’ IEL range has ever been published. In this multicentre study, receiver operating characteristic (ROC) curve analysis was used determine optimal cut-off between normal and CD (Marsh III lesion) duodenal mucosa, based on counts >400 mucosal biopsy specimens. Design The study designed at International Meeting Digestive Pathology, Bucharest 2015....