A5085481160- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Inflammatory Bowel Disease
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- TGF-β signaling in diseases
- RNA modifications and cancer
- Circular RNAs in diseases
- Gold and Silver Nanoparticles Synthesis and Applications
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Asthma and respiratory diseases
- Laser-Ablation Synthesis of Nanoparticles
- Cancer, Hypoxia, and Metabolism
- Neonatal Respiratory Health Research
- Helicobacter pylori-related gastroenterology studies
- Cell Adhesion Molecules Research
- Liver Diseases and Immunity
- Microscopic Colitis
- Vascular Anomalies and Treatments
- 3D Printing in Biomedical Research
University of Southampton
2014-2024
University Hospital Southampton NHS Foundation Trust
2016-2024
David Hide Asthma and Allergy Research Centre
2022-2024
NIHR Southampton Biomedical Research Centre
2017-2021
NIHR Southampton Respiratory Biomedical Research Unit
2019-2020
National Institute for Health Research
2019-2020
Southampton General Hospital
2009-2019
University of California, Riverside
2004-2006
Consejo Superior de Investigaciones Científicas
2000-2004
Centro de Investigaciones Biológicas Margarita Salas
2000-2004
Macrophages play a central role in the balance and efficiency of immune response are at interface between innate adaptive immunity. Their phenotype is delicate equilibrium M1 (classical, pro-Th(1)) M2 (alternative, pro-Th(2)) profiles. This regulated by cytokines such as interleukin 13 (IL-13), typical pro-M2-Th(2) cytokine that has been related to allergic disease asthma. IL-13 binds receptor α1 (IL13Rα1), component Type II IL-4 receptor, exerts its effects activating transcription factor...
High numbers of tissue-resident memory T (TRM) cells are associated with better clinical outcomes in cancer patients. However, the molecular characteristics that drive their efficient immune response to tumors poorly understood. Here, single-cell and bulk transcriptomic analysis TRM non-TRM present tumor normal lung tissue from patients revealed PD-1–expressing were clonally expanded enriched for transcripts linked cell proliferation cytotoxicity when compared cells. This feature was more...
Background The role of tumor-associated macrophages (TAMs) in determining the outcome between antitumor effects adaptive immune system and tumor’s anti-immunity stratagems, is controversial. Macrophages modulate their activities phenotypes by integration signals tumor microenvironment. Depending on how are activated, they may adopt so-called M1-like, or M2-like, protumor profiles. In many solid tumors, a dominance M2-like associated with poor outcomes but some types, strong M1-like profiles...
Abstract Background Intestinal macrophages are key immune cells in the maintenance of intestinal homeostasis and have a role pathogenesis inflammatory bowel disease (IBD). However, mechanisms by which exert pathological influence both ulcerative colitis (UC) Crohn (CD) not yet well understood. Methods We purified from gastrointestinal mucosal biopsies (patients with UC, patients CD, healthy donors) analyzed their transcriptome RNA sequencing bioinformatics, confirming results quantitative...
Signaling by transforming growth factor (TGF)-β family members is mediated Smad proteins that regulate gene transcription through functional cooperativity and association with other DNA-binding proteins. The hypoxia-inducible (HIF)-1 a transcriptional complex plays key role in oxygen-regulated expression. We demonstrate hypoxia TGF-β cooperate the induction of promoter activity vascular endothelial (VEGF), which major stimulus promotion angiogenesis. This cooperation has been mapped on human...
Homeotic genes contain cis-regulatory trithorax response elements (TREs) that are targeted by epigenetic activators and transcribed in a tissue-specific manner. We show the transcripts of three TREs located Drosophila homeotic gene Ultrabithorax (Ubx) mediate transcription activation recruiting regulator Ash1 to template TREs. TRE coincides with Ubx recruitment Drosophila. The SET domain binds all transcripts, each transcript hybridizing only corresponding chromatin. Transgenic restores...
Endoglin is an auxiliary component of the transforming growth factor-β (TGF-β) receptor system, able to associate with signaling types I (TβRI) and II (TβRII) in presence ligand modulate cellular responses TGF-β1. cannot bind on its own but requires receptors, supporting a critical role for interaction between endoglin TβRI or TβRII. This study shows that full-length interacts both TβRII, independently their kinase activation state exogenous Truncated constructs encoding either extracellular...
MicroRNA-155 (miR-155) has been involved in the response to inflammation macrophages and lymphocytes. Here we show how miR-155 participates maturation of human dendritic cells (DC) modulates pathogen binding by down-regulating DC-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN), after directly targeting transcription factor PU.1. During DCs, increases up 130-fold, whereas PU.1 protein levels decrease accordingly. We establish that is a direct target for localize...
Transforming growth factor-beta (TGF-β) is a pleiotropic cytokine with important effects on processes such as fibrosis, angiogenesis, and immunosupression. Using bioinformatics, we identified SMAD2, one of the mediators TGF-β signaling, predicted target for microRNA, microRNA-155 (miR-155). MicroRNAs are class small non-coding RNAs that have emerged an gene expression regulators. miR-155 has been found to be involved in regulation immune response myeloid cells. Here, provide direct evidence...
Abstract The interactions between noncancerous, primary endothelial cells and gold nanoparticles with different morphologies but the same ligand capping are investigated. incubated nanospheres, nanorods, hollow spheres, core/shell silica/gold nanocrystals, which coated monocarboxy (1‐mercaptoundec‐11‐yl) hexaethylene glycol (OEG). Cell viability studies show that all types of particles noncytotoxic. number taken up by is estimated using inductively coupled plasma (ICP), found to differ...
// Oliver Wood 1, * , Jeongmin Woo Gregory Seumois 3, Natalia Savelyeva 1 Katy J. McCann Divya Singh 3 Terry Jones 2 Lailah Peel Michael S. Breen Matthew Ward Eva Garrido Martin Tilman Sanchez-Elsner # Gareth Thomas Pandurangan Vijayanand Christopher H. Woelk Emma King Christian Ottensmeier for the SPARC Consortium Faculty of Medicine, University Southampton & Hospital Southampton, UK Department Molecular and Clinical Cancer Liverpool, La Jolla Institute Allergy Immunology, Jolla, CA,...
We demonstrate the deliberate activation or inhibition of invitro angiogenesis using functional peptide coated gold nanoparticles. The peptides, anchored to oligo-ethylene glycol capped nanospheres, were designed selectively interact with cell receptors responsible for angiogenesis. particles are shown influence significantly extent and morphology vascular structures, without causing toxicity. Mechanistic studies show that nanoparticles have ability alter balance between naturally secreted...
MicroRNAs are short non-coding single stranded RNAs that regulate gene expression. While much is known about the effects of individual microRNAs, there now growing evidence they can work in co-operative networks. to be dysregulated many diseases and affect pathways involved pathology. We investigated dysregulation microRNA networks using asthma as disease model. Asthma a chronic inflammatory airways characterized by bronchial hyperresponsiveness airway remodelling. The epithelium major...
Energy metabolism is intrinsic to cell viability but surprisingly has been little studied in human embryonic stem cells (hESCs). The current study aims investigate the effect of environmental O2 tension on carbohydrate utilisation hESCs. Highly pluripotent hESCs cultured at 5% consumed significantly more glucose, less pyruvate and produced lactate compared those maintained 20% O2. Moreover, atmospheric levels expressed OCT4, SOX2 NANOG than To determine whether this difference was a...
Asthma is one of the most common chronic diseases worldwide, and individuals with severe asthma experience recurrent exacerbations. Exacerbations are predominantly viral associated have been linked to defective airway IFN responses. Ascertaining molecular mechanisms underlying this deficiency a major research goal identify new therapeutic targets.We investigated hypothesis that reduced Toll-like receptor 7 (TLR7)-derived signaling drove impaired responses rhinovirus by asthmatic alveolar...
Abstract A new strategy to manipulate cell operations is demonstrated, based on membrane‐receptor‐specific interactions between colloidal peptide‐capped gold nanoparticles and human umbilical vein endothelial cells. It shown that of similar charge size but capped with different peptide sequences can deliberately trigger specific functions related the important biological process blood vessel growth known as angiogenesis. Specific binding particles two endothelial‐expressed receptors...
Low O2 tension is beneficial for human embryonic stem cell (hESC) maintenance but the mechanism of regulation unknown. HIF-2α was found to bind directly predicted hypoxic response elements (HREs) in proximal promoter OCT4, NANOG and SOX2 only hESCs cultured under hypoxia (5% O2). This binding induced an array histone modifications associated with gene transcription while a heterochromatic state existed at atmospheric O2. Interestingly, enhanced euchromatic when were exposed followed by 72...