A5067672031- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Biotin and Related Studies
- Pancreatic function and diabetes
- Click Chemistry and Applications
- Renal and related cancers
- RNA regulation and disease
- Sperm and Testicular Function
- Advanced Chemical Sensor Technologies
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Adenosine and Purinergic Signaling
- Caveolin-1 and cellular processes
- Reproductive Biology and Fertility
- Peroxisome Proliferator-Activated Receptors
- Molecular Biology Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- Helicobacter pylori-related gastroenterology studies
- Endoplasmic Reticulum Stress and Disease
- Photosynthetic Processes and Mechanisms
- Liver physiology and pathology
- Cancer Cells and Metastasis
- interferon and immune responses
University of Colorado Anschutz Medical Campus
2020-2025
Washington University in St. Louis
2016-2021
Cornell University
2014
We hypothesized that basic helix–loop–helix (bHLH) MIST1 (BHLHA15) is a “scaling factor” universally establishes secretory morphology in cells perform regulated secretion. Here, we show targeted deletion of caused dismantling the apparatus diverse exocrine cells. Parietal (PCs), whose function to pump acid into stomach, normally lack and do not Forced expression PCs them expand their apical cytoplasm, rearrange mitochondrial/lysosome trafficking, generate large granules. Mist1 induced cohort...
Abstract Thousands of RNA species display nonuniform distribution within cells. However, quantification the spatial patterns adopted by individual RNAs remains difficult, in part a lack quantitative tools for subcellular transcriptome analysis. In this study, we describe an proximity labeling method that facilitates populations with high specificity. This method, termed Halo-seq, pairs light-activatable, radical generating small molecule highly efficient Click chemistry to efficiently label...
Across cell types and organisms, thousands of RNAs display asymmetric subcellular distributions. Studying this process requires quantifying abundances specific at precise locations. To analyze transcriptomes, multiple proximity-based techniques have been developed in which near a localized bait protein are specifically labeled, facilitating their biotinylation purification. However, these complex methods often laborious require expensive enrichment reagents. streamline the analysis RNA...
Cellular RNA is asymmetrically distributed in cells and the regulation of localization crucial for proper cellular functions. However, limited chemical tools are available to capture dynamic complex biological systems with high spatiotemporal resolution. Here, we developed a new method proximity labeling activated by near-infrared (NIR) light, which holds potential deep penetration. Our method, termed FAP-seq, utilizes genetically encoded fluorogen activating protein (FAP) that selectively...
Summary The optimal markers for human spermatogonial stem cells ( SSC s) are not known. Among the genes recently linked to s in mice and other animals basic helix‐loop‐helix transcription factor ID 4 orphan G‐protein‐coupled receptor GPR 125. While 125 considered putative s, they have been evaluated coexpression tissue. Furthermore, neither size nor character of populations that express 125, respectively, A major barrier addressing these questions is availability healthy adult testis tissue...
RNAs encoding some centrosomal components are trafficked to the organelle during mitosis. Some RNAs, including
Human PRPF39 is a homolog of the yeast Prp39 and Prp42 paralogs. We have previously shown that human forms homodimer interacts with CTD U1C, mirroring Prp39/Prp42 heterodimer. demonstrate here knockdown in HEK293 cells affects many alternative splicing events primarily by reducing usage weak 5′ ss. Additionally, preferentially binds to GC-rich RNA, likely at interface between its NTD CTD. These data indicate potentially recruits U1 snRNP ss, serving as unrecognized factor. further TIA1 U1C...
Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The distribution of these proteins is often facilitated the localization RNA molecules encode them. A key question in study this process characterization transcripts present at a given location. Historically, experiments aimed answering have centered upon microscopy-based techniques target one or few time. However, more recently, advent high-throughput sequencing has allowed...
The gastric epithelium is often exposed to injurious elements and failure of appropriate healing predisposes ulcers, hemorrhage, ultimately cancer. We examined the function CD36, a protein linked disease homeostasis. used tamoxifen model injury in mice null for Cd36 (Cd36-/-), with deletion parietal cells (PC-Cd36-/-) or endothelial (EC-Cd36-/-). CD36 expresses on corpus ECs, PC basolateral membranes, gastrin ghrelin cells. Stomachs Cd36-/- have altered gland organization secretion, more...
Abstract The subcellular localization of specific RNA molecules promotes localized cellular activity across a variety species and cell types. misregulation this targeting can result in developmental defects, mutations proteins that regulate process are associated with multiple diseases. For the vast majority RNAs, however, mechanisms underlie their unknown, partly due to difficulty profiling quantifying populations. To address challenge, we developed Halo‐seq, proximity labeling technique...
ABSTRACT Thousands of RNA species display nonuniform distribution within cells. However, quantification the spatial patterns adopted by individual RNAs remains difficult, in part a lack quantitative tools for subcellular transcriptome analysis. In this study, we describe an proximity labeling method that facilitates populations with high specificity. This method, termed Halo-seq, pairs light-activatable, radical generating small molecule highly efficient Click chemistry to efficiently label...
Across cell types and organisms, thousands of RNAs display asymmetric subcellular distributions. The study this process often requires quantifying abundances specific at precise locations. To analyze transcriptomes, multiple proximity-based techniques have been developed in which near a localized bait protein are specifically labeled, facilitating their biotinylation purification. However, these complex methods laborious require expensive enrichment reagents. streamline the analysis RNA...