A5060635360- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Genetics and Neurodevelopmental Disorders
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
Harvard University
2022-2024
University of Michigan
2016-2024
Boston Children's Hospital
2022-2024
Michigan United
2017
RNA-mediated transcriptional gene silencing is a conserved process where small RNAs target transposons and other sequences for repression by establishing chromatin modifications. A central element of this are long non-coding (lncRNA), which in Arabidopsis thaliana produced specialized RNA polymerase known as Pol V. Here we show that transcription V controlled preexisting modifications located within the transcribed regions. Most transcripts associated with AGO4 but not sliced AGO4....
RNA-mediated transcriptional silencing, in plants known as RNA-directed DNA methylation (RdDM), is a conserved process where small interfering RNA (siRNA) and long non-coding (lncRNA) help establish repressive chromatin modifications. This represses transposons affects the expression of protein-coding genes. We found that Arabidopsis thaliana AGO4 binding sites are often located distant from genes differentially expressed ago4. Using Hi-C to compare interactions between genotypes, we show...
Significance Eukaryotic genomes are pervasively transcribed, yet most transcribed sequences lack conservation or known biological functions. We show that a specialized plant-specific RNA polymerase V broadly transcribes the Arabidopsis genome. propose model where Pol transcription surveils genome and is required to recognize repress newly inserted reactivated transposons. Our results indicate pervasive of nonconserved may serve an essential role in maintenance integrity.
Heritable non-genetic information can regulate a variety of complex phenotypes. However, what specific cues are transmitted from parents to their descendants poorly understood. Here, we perform metabolic methyl-labeling experiments track the heritable transmission methylation ancestors in nematode Caenorhabditis elegans (C. elegans). We find DNA, RNA, proteins, and lipids. that parental starvation elicits reduced fertility, increased heat stress resistance, extended longevity fed, naïve...
DNA is organized into chromatin-like structures that support the maintenance and regulation of genomes. A unique poorly understood form organization exists in chloroplasts, which are organelles endosymbiotic origin responsible for photosynthesis. Chloroplast genomes, together with associated proteins, membrane-less known as nucleoids. The internal arrangement nucleoid, molecular mechanisms organization, connections between nucleoid structure gene expression remain mostly unknown. We show
While heredity is predominantly controlled by what deoxyribonucleic acid (DNA) sequences are passed from parents to their offspring, a small but growing number of traits have been shown be regulated in part the non-genetic inheritance information. Transgenerational epigenetic defined as heritable information offspring without changing DNA sequence. Work past seven decades has transitioned was previously viewed rare phenomenology, into well-established paradigms which numerous can modulated....
Ribosome heterogeneity has emerged as an important regulatory control feature for determining which proteins are synthesized, however, the influence of age on ribosome is not fully understood. Whether mRNA transcripts selectively translated in young versus old cells and whether dysregulation this process drives organismal aging unknown. Here we examined role ribosomal RNA (rRNA) methylation maintaining appropriate translation organisms age. In a directed RNAi screen, identified 18S rRNA...
Abstract Non-coding transcription is an important determinant of heterochromatin formation. In Arabidopsis thaliana a specialized RNA polymerase V (Pol V) transcribes pervasively and produces long non-coding RNAs. These transcripts work with small interfering to facilitate locus-specific establishment RNA-directed DNA methylation (RdDM). Subsequent maintenance RdDM associated elevated levels Pol transcription. However, the impact on remained unresolved. We found that strongly enhances The...
ABSTRACT DNA is organized into chromatin-like structures, which support the maintenance and regulation of genomes. A unique poorly understood form packaging exists in chloroplasts, are endosymbiotic organelles responsible for photosynthesis. Chloroplast genomes, together with associated proteins, membraneless structures known as nucleoids. The internal arrangement nucleoid, molecular mechanisms packaging, connections between nucleoid structure gene expression remain mostly unknown. We show...
<title>Abstract</title> Ribosome heterogeneity has emerged as an important regulatory control feature for determining which proteins are synthesized, however, the influence of age on ribosome is not fully understood. Whether mRNA transcripts selectively translated in young versus old cells and whether dysregulation this process drives organismal aging unknown. Here we examined role ribosomal RNA (rRNA) methylation maintaining appropriate translation organisms age. In a directed RNAi screen,...
ABSTRACT Repressive chromatin modifications are instrumental in regulation of gene expression and transposon silencing. In Arabidopsis thaliana , transcriptional silencing is performed by the RNA-directed DNA methylation (RdDM) pathway. this process, two specialized RNA polymerases, Pol IV V, produce non-coding RNAs, which recruit several RNA-binding proteins lead to establishment repressive marks. An important feature nucleosome positioning, has also been implicated RdDM. We show that RdDM...
Abstract Post-translational modifications of histone tails alter chromatin accessibility to regulate gene expression. Some viruses exploit the importance by expressing mimetic proteins that contain histone-like sequences sequester complexes recognize modified histones. Here we identify an evolutionarily conserved and ubiquitously expressed, endogenous mammalian protein Nucleolar 16 (NOP16) functions as a H3K27 mimic. NOP16 binds EED in trimethylation PRC2 complex demethylase JMJD3. knockout...