A5057599524- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Reproductive Biology and Fertility
- Molecular Biology Techniques and Applications
- Plant and Fungal Interactions Research
- RNA Research and Splicing
- Plant-Microbe Interactions and Immunity
- Genomics and Chromatin Dynamics
- Biofuel production and bioconversion
- Mechanisms of cancer metastasis
- Plant Pathogenic Bacteria Studies
- Chromatin Remodeling and Cancer
- Nematode management and characterization studies
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Nuts composition and effects
- Cancer Cells and Metastasis
- Legume Nitrogen Fixing Symbiosis
- Prenatal Screening and Diagnostics
- Peptidase Inhibition and Analysis
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
Boston Children's Hospital
2023-2025
Harvard University
2023-2025
Instituto de Investigaciones Biológicas Clemente Estable
2015-2024
Abstract Cancers display cellular, genetic and epigenetic heterogeneity, complicating disease modeling. Multiple cell states defined by gene expression have been described in lung adenocarcinoma (LUAD). However, the functional contributions of state regulatory programs that control chromatin early stages tumor initiation are not well understood. Using single-cell RNA ATAC sequencing Kras/p53-driven organoids, we identified two major cellular states: one more closely resembling alveolar type...
The discovery of a large number long noncoding RNAs (lncRNAs), and the finding that they may play key roles in different biological processes, have started to provide new perspective understanding gene regulation. It has been shown testes express highest amount lncRNAs among vertebrate tissues. However, although some studies addressed characterization along spermatogenesis, an exhaustive analysis differential expression at its stages is still lacking. Here, we present results for lncRNA...
More than 50% of cases primary ovarian insufficiency (POI) and nonobstructive azoospermia in humans are classified as idiopathic infertility. Meiotic defects may relate to at least some these cases. Mutations genes coding for synaptonemal complex (SC) components have been identified humans, hypothesized be causative the observed infertile phenotype. Mutation SYCE1 c.721C>T (former c.613C>T)-a familial mutation reported two sisters with amenorrhea-was first such found an SC central element...
Abstract Background Mammalian testis is a highly complex and heterogeneous tissue. This complexity, which mostly derives from spermatogenic cells, reflected at the transcriptional level, with largest number of tissue-specific genes long noncoding RNAs (lncRNAs) compared to other tissues, one highest rates alternative splicing. Although it known that adequate alternative-splicing patterns stage-specific isoforms are critical for successful spermatogenesis, so far only very limited reports...
Herbaspirillum seropedicae Z67 is a diazotrophic endophyte able to colonize the interior of many economically relevant crops such as rice, wheat, corn and sorghum. Under iron-deficient conditions, this organism secretes serobactins, suite lipopetide siderophores. The role siderophores in interaction between endophytes their plant hosts are not well understood. In work, we aimed determine importance serobactins-mediated iron acquisition systems H. with rice plants. First provide evidence, by...
Herbaspirillum seropedicae Z67 is a nitrogen-fixing endophyte that colonizes many important crops. Like in almost all organisms, vital cellular processes of this are iron dependent. In order to efficiently acquire fulfill its requirements, bacterium produces the siderophores serobactins. However, presence genome others acquisition genes suggests serobactins not only strategy used by H. circumvent metal deficiency. The aim work was identify and proteins differentially expressed cells growing...
Abstract Objective To investigate if nonsense mutation SYCE1 c.613C˃T - found in women with familial primary ovarian insufficiency (POI)- is actually responsible for infertility, and to elucidate the involved molecular mechanisms. Design As most fundamental mammalian oogenesis events occur during embryonic phase, thus hindering study of POI’s etiology/pathogeny infertile women, we have used CRISPR/Cas9 technology generate a mouse model line an equivalent genome alteration (humanized mice)....
Abstract Post-translational modifications of histone tails alter chromatin accessibility to regulate gene expression. Some viruses exploit the importance by expressing mimetic proteins that contain histone-like sequences sequester complexes recognize modified histones. Here we identify an evolutionarily conserved and ubiquitously expressed, endogenous mammalian protein Nucleolar 16 (NOP16) functions as a H3K27 mimic. NOP16 binds EED in trimethylation PRC2 complex demethylase JMJD3. knockout...
Although the portion of genome devoted to protein synthesis is very small, it now known that almost entire expressed as non-coding RNAs. Among them, there are long noncoding RNAs (lncRNAs). Despite lncRNAs have been poorly studied, they recently started focus attention researchers, has found out can perform diverse functions in regulation gene expression. Besides, their involvement pathologies being revealed. Intriguingly, amount testis overwhelmingly higher than any other analyzed organ or...
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