A5003965477- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Reproductive Biology and Fertility
- Nutrition, Genetics, and Disease
- RNA Research and Splicing
- Sperm and Testicular Function
- Hereditary Neurological Disorders
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Carcinogens and Genotoxicity Assessment
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Toxin Mechanisms and Immunotoxins
- Cancer therapeutics and mechanisms
- Plant Disease Resistance and Genetics
- Genetically Modified Organisms Research
- Plant Virus Research Studies
- Cancer-related molecular mechanisms research
- Telomeres, Telomerase, and Senescence
- Nuclear Structure and Function
- RNA modifications and cancer
- Origins and Evolution of Life
Instituto de Investigaciones Biológicas Clemente Estable
2011-2020
Universidad de la República
2007-2019
University of Duisburg-Essen
1998
Spermatogenesis is a complex differentiation process that involves the successive and simultaneous execution of three different gene expression programs: mitotic proliferation spermatogonia, meiosis, spermiogenesis. Testicular cell heterogeneity has hindered its molecular analyses. Moreover, characterization short, poorly represented stages such as initial meiotic prophase ones (leptotene zygotene) remained elusive, despite their crucial importance for understanding fundamentals meiosis. We...
Poly- adenosine diphosphate (ADP)-ribose (PAR) is a polymer synthesized as posttranslational modification by some poly (ADP-ribose) polymerases (PARPs), namely PARP-1, PARP-2, tankyrase-1, and tankyrase-2 (TNKS-1/2). PARP-1 nuclear has also been detected in extracellular vesicles. PARP-2 TNKS-1/2 are distributed nuclei cytoplasm. PARP or PAR alterations have described tumors, particular influencing the Epithelial- Mesenchymal Transition (EMT), which influences cell migration drug resistance...
The discovery of a large number long noncoding RNAs (lncRNAs), and the finding that they may play key roles in different biological processes, have started to provide new perspective understanding gene regulation. It has been shown testes express highest amount lncRNAs among vertebrate tissues. However, although some studies addressed characterization along spermatogenesis, an exhaustive analysis differential expression at its stages is still lacking. Here, we present results for lncRNA...
Availability of purified or highly enriched fractions representing the various spermatogenic stages is a usual requirement to study mammalian spermatogenesis at molecular level. Fast preparation high quality testicular cell suspensions crucial when flow cytometry (FCM) chosen accomplish stage/s purification. Formerly, we reported method rapidly obtain good rodent for FCM analysis and sorting. Using that could distinguish purify early meiocytes (leptotene/zygotene stages, L/Z) from more...
Homogeneity of cell populations is a prerequisite for the analysis biochemical and molecular events during male gamete differentiation. Given complex organization mammalian testicular tissue, various methods have been used to obtain enriched or purified populations, including flow sorting. Current protocols are usually time-consuming may imply loss short-lived RNAs, which undesirable expression profiling. We describe an optimized method speed up preparation suitable suspensions cytometric...
Mucins participate in cancer progression by regulating cell growth, adhesion, signaling, apoptosis or chemo-resistance to drugs. The secreted mucin MUC5B, the major component of respiratory tract mucus, is aberrantly expressed breast cancer, where it could constitute a biomarker. In this study we evaluated role MUC5B gene silencing expression with short hairpin RNA on MCF-7 cells. We found that MUC5B-silenced cells have reduced capacity grow, adhere and form colonies. Interestingly,...
Abstract Mammalian spermatogenesis is still nowadays poorly understood at the molecular level. Testis cellular heterogeneity a major drawback for spermatogenic gene expression studies, especially when research focused on stages that are usually very short and represented level such as initial meiotic prophase I (i.e., leptotene [L] zygotene [Z]). Presumably, genes whose products involved in critical events alignment, pairing recombination of homologous chromosomes expressed during early...
Mammalian testes are very complex organs that contain over 30 different cell types, including somatic testicular cells and stages of germline cells. This heterogeneity is an important drawback concerning the study bases mammalian spermatogenesis, as pure or enriched populations in certain sperm development needed for most molecular analyses. Various strategies such Staput, centrifugal elutriation, flow cytometry (FC) have been employed to obtain purified order enable differential gene...
Intact Chinese hamster ovary cells were exposed to the restriction endonucleases (REs) AluI or BamHI. In metaphase spreads from these cells, 300 breakpoints per RE localized in G-banded chromosome type aberrations (dicentrics, translocations, rings, terminal and interstitial deletions). The majority of induced by both REs G-light bands showed a similar distribution breakpoint clusters. digestion with C-banding, BamHI G-banding. data indicate that nuclease sensitive sites associated active...
Many <i>Achyrocline</i> (Asteraceae, tribe Gnaphalieae) species are widely used in Argentina, Brazil and Uruguay as popular medicinal aromatic plants. <i>Achyrocline flaccida</i>, <i>A. satureioides</i>, alata</i>, crassiuscula</i> distributed popularly known ‘marcelas’. In order to characterize them, we performed chromosome counts, compared the karyotypes, mapped 5S 45S rDNA sites by fluorescent situ hybridization, estimated their DNA content....
Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal regenerating peripheral nerves. Previously, we have shown that transfer is dependent on F-actin cytoskeleton Myosin Va. Here, explored the contribution microtubules to newly synthesized transport cell nuclei up nodal microvilli sciatic Results using immunohistochemistry quantitative confocal FRET analysis indicate cell-derived co-localize with cytoplasm. Additionally, nocodazole colchicine sensitive...
SORB (selected observed residual breakpoints) induced by ionizing radiation or endonucleases are often non-randomly distributed in mammalian chromosomes. However, the role played chromatin structure localization of chromosome is not well understood. Anti-topoisomerase drugs such as etoposide potent clastogens and unlike radiation, induce DNA double-strand breaks (DSB) an indirect mechanism. Topoisomerase II (Topo II) a main component nuclear matrix scaffold. Since leads to DSB influencing...
The restriction endonucleases (REs) Alu I and Bam HI were electroporated into Chinese hamster ovary (CHO) cells during S phase of the cell cycle breakpoints in G-banded metaphases mapped to Giemsa-light ordark bands or band junctions. majority I- HI-induced located bands. Both REs induced similar distributions breakpoint clusters. localization pattern phase-induced CHO is G 1-induced reported earlier. These data show that for both independent stage (G 1 S) which aberrations are give further...
Trithrinax campestris (Burmeist.) Drude & Griseb., a neotropical member of tribe Cryosophileae, subfamily Coryphoideae (Arecaceae), forms small populations in the southern most extreme distribution for tribe. To provide genetic information to assist its conservation, we performed detailed karyotype analysis samples from Uruguay. The species displayed with 2n = 36, genome size (2C 17.15 ± 0.07 pg) exceptionally high palm tree, and an elevated heterochromatin amount, represented by eight...
Purpose: Non-random occurrence of induced chromosome breakpoints (BP) has been repeatedly reported. DNA synthesis and chromatin remodeling may influence BP localization. The CHO9 X exhibits an early replicating short euchromatic arm (Xpe) a late long heterochromatic (Xqh). We investigated the role played by replication related processes on distribution in eu/heterochromatin using as model.