A5016785196- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- interferon and immune responses
- RNA Research and Splicing
- RNA regulation and disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related Molecular Pathways
- Extracellular vesicles in disease
Instituto de Investigaciones Biológicas Clemente Estable
2013-2024
Ministerio de Educación y Cultura
2024
Universidad de la República
2024
Introduction: Astrocytes are the glial cells responsible for brain homeostasis, but if injured, they could damage neural even deadly. Genetic damage, DNA response (DDR), and its downstream cascades dramatic events poorly studied in astrocytes. Hypothesis methods: We propose that 1 h of 400 mmol/L ethanol and/or μmol/L corticosterone exposure cultured hippocampal astrocytes damages DNA, activating DDR eliciting functional changes. Immunolabeling against γH2AX (chromatin sites), cyclin D1...
In eukaryotes, a cascade of events named DNA damage response (DDR) has evolved to handle lesions. DDR engages the recruitment signaling, checkpoint control, repair and chromatin remodeling protein complexes, allowing cell cycle delay, or induction apoptosis. An early event involves phosphorylation histone variant γH2AX on serine 139 (H2AX139 phosphorylation) originating so-called γH2AX. DDR-related H2AX139 have been extensively studied in interphase nuclei. More recently, signals mitotic...
Astrocytes maintain CNS homeostasis but also critically contribute to neurological and psychiatric disorders. Such functional diversity implies an extensive signaling repertoire including extracellular vesicles (EVs) nanotubes (NTs) that could be involved in protection or damage, as widely shown various experimental paradigms. However, there is no information associating primary damage the astrocyte genome, DNA response (DDR), EV NT repertoire. Furthermore, similar studies were not performed...