A5012344248- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Neurological diseases and metabolism
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- RNA regulation and disease
California Baptist University
2023-2024
University of California, Riverside
2003-2020
Abstract Cyt1Aa is the one of four crystalline protoxins produced by mosquitocidal bacterium Bacillus thuringiensis israelensis ( Bti ) that has been shown to delay evolution insect resistance in field. Limiting our understanding efficacy and path improved toxicity spectrum ignorance how crystallizes vivo its mechanism toxicity. Here, we use serial femtosecond crystallography determine protoxin structure from sub-micron-sized crystals . Structures determined under various pH/redox conditions...
The Lysinibacillus sphaericus proteins Tpp49Aa1 and Cry48Aa1 can together act as a toxin toward the mosquito Culex quinquefasciatus have potential use in biocontrol. Given that with sequence homology to individual activity alone against other insect species, structure of was solved order understand this protein more fully inform design improved biopesticides. is naturally expressed crystalline inclusion within host bacterium, MHz serial femtosecond crystallography using novel nanofocus...
Mosquitoes are the most notorious arthropod vectors of viral and parasitic diseases for which approximately half world’s population, ~4,000,000,000, is at risk. Integrated pest management programs (IPMPs) have achieved some success in mitigating regional transmission persistence these diseases. However, as many vector-borne remain pervasive, it obvious that IPMP successes not been absolute eradicating threat imposed by mosquitoes. Moreover, expanding mosquito geographic ranges caused factors...
The three most important commercial bacterial insecticides are all derived from subspecies of Bacillus thuringiensis (Bt). Specifically, Bt subsp. kurstaki (Btk) and aizawai (Bta) used to control larval lepidopteran pests. third, israelensis (Bti), is primarily mosquito blackfly larvae. All produce a parasporal body (PB) during sporulation. PB composed insecticidal proteins that damage the midgut epithelium, initiating complex process results in death insect. Among these Bt, Bti unique as it...