A5103206105- Angiogenesis and VEGF in Cancer
- Spine and Intervertebral Disc Pathology
- Magnetic and Electromagnetic Effects
- Musculoskeletal pain and rehabilitation
- Microbial Inactivation Methods
- Sarcoma Diagnosis and Treatment
- Circular RNAs in diseases
- Bone health and treatments
- Proteoglycans and glycosaminoglycans research
- Bone health and osteoporosis research
- TGF-β signaling in diseases
- Tendon Structure and Treatment
- Probiotics and Fermented Foods
- Spinal Fractures and Fixation Techniques
- Medical Imaging and Analysis
- Bone Metabolism and Diseases
- Identification and Quantification in Food
- Osteoarthritis Treatment and Mechanisms
- Cancer Cells and Metastasis
- Cervical and Thoracic Myelopathy
- Meat and Animal Product Quality
- Medical Research and Treatments
- Food Allergy and Anaphylaxis Research
- Advanced Chemical Sensor Technologies
- Digestive system and related health
University of Yamanashi
2008-2021
University of Yamanashi Hospital
2015
Vanderbilt University Medical Center
2014
ABSTRACT Osteosarcoma is the most common primary malignant tumor of bone and accounts for around 50% all skeletal malignancies. In addition to novel chemotherapies, there a need adjuvant therapies designed inhibit osteosarcoma proliferation tumor-induced osteolysis attenuate expansion metastasis. As such, studies on efficacy bisphosphonates human are planned after feasibility determined that bisphosphonate zoledronic acid (ZOL) can be safely combined with conventional chemotherapy. However,...
Abstract Osteosarcoma is the most common primary bone malignancy and accounts for more than half of skeletal malignancies in children young adults. Although vascular endothelial growth factor (VEGF) expression osteosarcoma has been associated with poor outcome, its role pathogenesis remains controversial. Here, VEGF VEGFR1 both human murine cells increasing malignant potential. Autocrine VEGF/VEGFR1 signaling resulted constitutive activation highly aggressive cells. In addition, survival...
Osteoarthritis (OA) is a group of common, chronic, and painful inflammatory joint diseases. One important finding in OA patients remarkable decrease the molecular weight hyaluronic acid (HA) synovial fluid affected joints. Therapeutic HA available to most parts world as viscosupplementation product for treatment OA. Previous clinical reports show that high (HMWHA) more effectively relieves pain than low (LMWHA). However, mechanism behind this remains unclear. In study, we investigated...
Radiographic imaging plays a crucial role in the diagnosis of osteosarcoma. Currently, computed-tomography (CT) is used to measure tumor-induced osteolysis as marker for tumor growth by monitoring bone fractional volume. As most tumors primarily induce osteolysis, lower volume has been found correlate with aggressiveness. However, osteosarcoma an exception it induces and produces mineralized osteoid simultaneously. Given that competent highly anisotropic (systematic variance its...
Metabolome analysis and physicochemical analyses were executed with cell extracts of a Lactococcus lactis subspecies cremoris strain treated by moderate pulsed electric field (PEF) to elucidate the mechanism enhanced production exopolysaccharide (EPS) treatment. capillary electrophoresis time flight mass spectrometry annotated 224 metabolites from cytoplasmic extract strain, which, however, showed no significant changes in related EPS production. Electron microscopic observation chemical...
Tree nuts comprise a category of food allergens that must be included in the labels several countries. We developed polymerase chain reaction (PCR) method using eight specific primer pairs to detect representative tree (almond, Brazil nut, cashew, hazelnut, macadamia pecan, pistachio, and walnut) under same experimental conditions. The specificity was confirmed by PCR testing against variety plant animal samples. detection limit ranged from 1 fg pg DNA individual nuts. detected nut processed...
<p>PDF - 1009K, S3. Chemotherapy-naive (Osteosarcoma 2) or adjuvant treated 3-10) human 25 osteosarcoma tissue was stained by immunofluorescence for VEGF (green) VEGFR1 (red). 26 Merged images (yellow) demonstrate co-expression of VEGFR and in a subpopulation 27 cells (magnification x200; scale bar, 100microm).</p>
<p>PDF - 80K, Supplementary Figure Legends</p>
<p>S2. (A) K7M3 cells (1x105 cell/well) treated with anti-VEGF164 antibody or VEGFR1 kinase inhibitor were seeded in the upper layer of a tranwell incubation chamber without 10% FBS added to lower and incubated for 24 hours. Non-migrating removed from filter was fixed stained crystal violet (magnification x100, scale bar indicates 500 microm). (B) Absorbance quantified at 590nm (OD 590). Data represent mean (plus minus) SD two independent experiments (*, P < 0.01).</p>
<p>PDF - 1009K, S3. Chemotherapy-naive (Osteosarcoma 2) or adjuvant treated 3-10) human 25 osteosarcoma tissue was stained by immunofluorescence for VEGF (green) VEGFR1 (red). 26 Merged images (yellow) demonstrate co-expression of VEGFR and in a subpopulation 27 cells (magnification x200; scale bar, 100microm).</p>
<p>PDF - 80K, Supplementary Figure Legends</p>
<div>Abstract<p>Osteosarcoma is the most common primary bone malignancy and accounts for more than half of skeletal malignancies in children young adults. Although vascular endothelial growth factor (VEGF) expression osteosarcoma has been associated with poor outcome, its role pathogenesis remains controversial. Here, VEGF VEGFR1 both human murine cells increasing malignant potential. Autocrine VEGF/VEGFR1 signaling resulted constitutive activation highly aggressive cells. In...
In past research, we have developed a telescopic rod system that expands and contracts inside the human body can be used for spinal fusion. The expanded contracted as wearer grows changes in symptoms by applying ultrasonic vibration. consists of instruments actual fusion, special metal rods, nuts. tightening torque locking cap secures to spine movement system. this paper, show verification effect on rod. Verification was performed water using an probe. From experimental results, outside...