- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Fungal Plant Pathogen Control
- Plant Pathogenic Bacteria Studies
- Plant pathogens and resistance mechanisms
- Mycotoxins in Agriculture and Food
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Management Techniques
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Insect Resistance and Genetics
- Banana Cultivation and Research
- Bacterial biofilms and quorum sensing
- Forest Insect Ecology and Management
- Essential Oils and Antimicrobial Activity
- Wheat and Barley Genetics and Pathology
- Allelopathy and phytotoxic interactions
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Genetics and Plant Breeding
- Powdery Mildew Fungal Diseases
- Entomopathogenic Microorganisms in Pest Control
Nanjing Agricultural University
2018-2022
University of Agriculture Faisalabad
2016-2022
Islamia University of Bahawalpur
2021
Nanjing Institute of Agricultural Mechanization
2019
University of Faisalabad
2017
To develop an effective biological agent to control Sclerotinia sclerotiorum, three endophytic Bacillus spp. strains with high antagonistic activity were isolated from maize seed and characterized. In vitro assays revealed that the endophytes could produce volatile organic compounds (VOC) reduced sclerotial production inhibited mycelial growth of S. sclerotiorum. Gas chromatography–mass spectrometry selected produced 16 detectable VOC. Eight VOC exhibited negative effects on while a further...
Fusarium graminearum is a notorious pathogen that causes head blight (FHB) in cereal crops. It produces secondary metabolites, such as deoxynivalenol, diminishing grain quality and leading to lesser crop yield. Many strategies have been developed combat this pathogenic fungus; however, considering the lack of resistant cultivars likelihood environmental hazards upon using chemical pesticides, efforts shifted toward biocontrol plant diseases, which sustainable eco-friendly approach. Fengycin,...
Lipopeptides from Bacillus species exhibit promising biological control activity against plant pathogens. This study aimed to explore the potential of purified fengycin induce systemic resistance in tomato Sclerotinia sclerotiorum. amyloliquefaciens FZB42, its mutant AK1S, and their corresponding metabolites showed vitro inhibition S. sclerotiorum mycelium. Fengycin derived an AK1S was identified through HPLC MALDI-TOF-MS, respectively. Scanning electron microscopy (SEM) transmission (TEM)...
Abiotic stress in plants pose a major threat to cereal crop production worldwide and cold is also notorious for causing decrease plant growth yield wheat. The present study was designed alleviate on by inoculating psychrophilic PGPR bacteria belonging Bacillus genera isolated from extreme rhizospheric environments of Qinghai-Tibetan plateau. genetic screening spp. CJCL2, RJGP41 temperate B. velezensis FZB42 revealed presence features corresponding response, membrane transport, signal...
Bacillus volatiles to control plant nematodes is a topic of great interest among researchers due its safe and environmentally friendly nature. strain GBSC56 isolated from the Tibet region China showed high nematicidal activity against M. incognita, with 90% mortality as compared in partition plate experiment. Pure produced by were identified through gas chromatography mass spectrometry (GC-MS). Among 10 volatile organic compounds (VOCs), 3 volatiles, i.e., dimethyl disulfide (DMDS), methyl...
The potential of endophytic Bacillus strains to improve plant growth and yield was evaluated.Endophytic altitudinis HNH7 velezensis HNH9 were evaluated for their growth-promoting traits. In an in vitro plate assay, exhibited proteolytic, amylolytic, lipolytic cellulolytic activity. able solubilize iron by producing siderophores but unable insoluble phosphate. PCR confirmed the presence four genes viz. pvd, budA, asbA satA genome HNH7, while also possessed same except budA. a greenhouse...
Verticillium wilt caused by
ABSTRACTBeing sessile organisms, plants are continuously challenged by phytopathogenic fungi, contributing the largest share in loss due to plant disease. Plants naturally possess a well-developed and programmed protein-based defense system, capable of producing antimicrobial cationic peptides ward off pathogen attack. Numerous genes encoding antifungal proteins have been isolated, cloned, sequenced transgenically expressed against multiple fungi successfully. Genetic engineering technology...
Due to its topographical position and climatic conditions, the Qinghai-Tibet Plateau possesses abundant microorganism resources. The extremophilic strain KKD1 isolated from Hoh Xil strong stress tolerance, enabling it propagate under high salinity (13%) alkalinity (pH 10.0) conditions. In addition, exhibits promising biocontrol activity against plant pathogens. To further explore these traits at genomic level, we performed whole-genome sequencing analysis. taxonomic identification according...
Sclerotinia sclerotiorum is a devastating necrotrophic pathogen that infects multiple crops, and its control an unremitting challenge. In this work, we attempted to gain insights into the pivotal role of lipopeptides (LPs) in antifungal activity Bacillus amyloliquefaciens EZ1509. comparative study involving five strains, B. EZ1509 harboring four LPs biosynthetic genes (viz. surfactin, iturin, fengycin, bacilysin) exhibited promising against S. dual-culture assay. Our data demonstrated...
This study elaborates inter-kingdom signaling mechanisms, presenting a sustainable and eco-friendly approach to combat biotic as well abiotic stress in wheat. Fusarium graminearum is devastating pathogen causing head seedling blight wheat, leading huge yield economic losses. Psychrophilic Bacillus atrophaeus strain TS1 was found potential biocontrol agent for suppression of F. under low temperature by carrying out extensive biochemical molecular studies comparison with temperate model...
Fusarium oxysporum f. sp. lycopersici induces wilt disease in tomato (Solanum lycopersicum L.), causing large loss yield quality and quantity. In vivo vitro studies were conducted to evaluate effects of natural, cheaply available cow, horse, chicken, or sheep manure the antagonistic isolates Trichoderma viride T. harzianum against F. on cv. Money Maker. The combination cow performed best controlling incidence tomato. Both evaluated individually combination. mix produced pathogen inhibition....
In vitro antifungal potential of Trichoderma isolates, selective botanical extracts and fungicides against A. solani was evaluated. i.e. T. harzianum, viride hamatum, were tested for their effect by dual culture technique at 48, 96, 144 172 hrs. hamatum produced the highest inhibition in vitro, followed hazianum after Methanolic leaf Elettaria cardamomum, Syzygium aromaticum, Curcuma longa root extract Parthenium hysterophorus showed up to 100% solani, compared control, while methanolic stem...
Many species of plant-pathogenic gram-negative bacteria deploy the type III (T3) secretion system to secrete virulence components, which are mostly characteristic protein effectors targeting cytosol plant cell following secretion. Xanthomonas oryzae pv. (Xoo), a rice pathogen causing bacterial blight disease, uses T3 accessory HrpE assemble pilus pathway, in turn secretes transcription activator-like (TAL) effectors. The hrpE gene can execute extensive physiological and pathological...