A5049550241- Plant Molecular Biology Research
- RNA Research and Splicing
- Plant Virus Research Studies
- RNA regulation and disease
- interferon and immune responses
- RNA Interference and Gene Delivery
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Photosynthetic Processes and Mechanisms
- Nitrogen and Sulfur Effects on Brassica
- Chromosomal and Genetic Variations
- Silkworms and Sericulture Research
- Plant Disease Resistance and Genetics
- Phytoplasmas and Hemiptera pathogens
- Cancer-related molecular mechanisms research
Architecture et Réactivité de l'arN
2018-2021
Université de Strasbourg
2016-2020
Centre National de la Recherche Scientifique
2013-2020
Institut de Biologie Moléculaire et Cellulaire
2020
Institut de Biologie Moléculaire des Plantes
2013-2018
The antiviral innate immune response mainly involves type I interferon (IFN) in mammalian cells. contribution of the RNA silencing machinery remains to be established, but several recent studies indicate that ribonuclease DICER can generate viral siRNAs specific conditions. It has also been proposed IFN and could mutually exclusive responses. In order decipher implication during infection human cells with alphaviruses such as Sindbis virus Semliki forest virus, we determined its interactome...
Article19 August 2020Open Access Prevention of dsRNA-induced interferon signaling by AGO1x is linked to breast cancer cell proliferation Souvik Ghosh orcid.org/0000-0003-4152-4460 Computational and Systems Biology, Biozentrum, University Basel, Switzerland Search for more papers this author Joao C Guimaraes Corresponding Author [email protected] orcid.org/0000-0002-1664-472X Manuela Lanzafame Institute Pathology, Hospital Alexander Schmidt Proteomics Core Facility, Afzal Pasha Syed Beatrice...
RNA silencing is a natural defence mechanism against viruses in plants, and transgenes expressing viral RNA-derived sequences were previously shown to confer silencing-based enhanced resistance the cognate virus several species. However, was dysfunction at low temperatures species, questioning relevance of this strategy perennial plants such as grapevines, which are often exposed during winter season. Here, we show that inverted-repeat (IR) constructs trigger highly efficient reaction all...
In plants, several dsRNA-binding proteins (DRBs) have been shown to play important roles in various RNA silencing pathways, mostly by promoting the efficiency and/or accuracy of Dicer-like (DCL)-mediated small production. Among DRBs encoded Arabidopsis genome, we recently identified DRB7.2 whose function was unknown. Here, show that is specifically involved siRNA production from endogenous inverted-repeat (endoIR) loci. This requires its interacting partner DRB4, main cofactor DCL4 and...
Summary In the model plant Arabidopsis thaliana , four Dicer‐like proteins ( DCL 1–4) mediate production of various classes small RNA s sRNA s). Among these proteins, 4 is by far most versatile RN ase III ‐like enzyme, and previously identified dcl4 missense alleles were shown to uncouple 4‐dependent s. Yet little known about molecular mechanism behind this uncoupling. Here, studying subcellular localization, interactome binding precursors three distinct alleles, we simultaneously highlight...
Double-stranded RNA binding (DRB) proteins are generally considered as promoting cofactors of Dicer or Dicer-like (DCL) that ensure efficient and precise production small RNAs, the sequence-specificity guide silencing processes in both plants animals. However, characterization a new clade DRB Arabidopsis has recently challenged this view by showing DRBs can also act potent inhibitors DCL processing. This is achieved through sequestration specific class precursors, endogenous inverted-repeat...
Abstract The antiviral innate immune response mainly involves type I interferon (IFN) in mammalian cells. contribution of the RNA silencing machinery remains to be established, but several recent studies indicate that ribonuclease DICER can generate viral siRNAs specific conditions. It has also been proposed IFN and could mutually exclusive responses. In order decipher implication during infection human cells with alphaviruses such as Sindbis virus Semliki forest virus, we determined its...
RNA silencing is a small based mechanism regulating gene expression and involved in many biological processes most eukaryotes. In plants, nematodes arthropods, this participates to antiviral defense. mammals, although the machinery present needed for microRNA pathway, its importance as an defense still debated. recent years, several studies have attempted answer question of whether pathway retained mammals. However, these did not provide clear yet. review, we will arguments against relevant...