A5004498216- RNA modifications and cancer
- RNA Research and Splicing
- MicroRNA in disease regulation
- Microtubule and mitosis dynamics
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Microfluidic and Capillary Electrophoresis Applications
- Genomics and Chromatin Dynamics
- Gene Regulatory Network Analysis
- Photosynthetic Processes and Mechanisms
- Microfluidic and Bio-sensing Technologies
- Lung Cancer Treatments and Mutations
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrostatics and Colloid Interactions
- Cellular transport and secretion
- HER2/EGFR in Cancer Research
- Chromatin Remodeling and Cancer
- Glycosylation and Glycoproteins Research
- Mechanisms of cancer metastasis
- Education Practices and Challenges
Middle East Technical University
2013-2023
Ondokuz Mayıs University
2023
University of Cambridge
2023
Jewish General Hospital
2023
Inonu University
2010
University of Michigan–Ann Arbor
2001-2003
Medical University of Vienna
2001
Institut Curie
2001
Centre National de la Recherche Scientifique
2001
Radiation Effects Research Foundation
2001
Non-syndromic low frequency sensorineural hearing loss (LFSNHL) affecting only 2000 Hz and below is an unusual type of that worsens over time without progressing to profound deafness. This LFSNHL may be associated with mild tinnitus but not vertigo. We have previously reported two families autosomal dominant linked adjacent non-overlapping loci on 4p16, DFNA6 DFNA14. However, further study revealed individual in the family who had a recombination event excluded DFNA14 candidate region was...
MicroRNAs (miRNAs), are ∼22 nucleotides long, non-coding RNAs that control gene expression post-transcriptionally by binding to their target mRNA's 3′UTRs (untranslated regions). Due roles in various important regulatory processes and pathways, miRNAs have been implicated disease mechanisms such as tumorigenesis when is deregulated. To date, a significant number of messenger (mRNAs) identified verified. It generally accepted can potentially bind many mRNAs, which brings the requirement...
3'-Untranslated region (UTR) shortening of mRNAs via alternative polyadenylation (APA) has important ramifications for gene expression. By using proximal APA sites and switching to shorter 3'-UTRs, proliferating cells avoid miRNA-mediated repression. Such 3'-UTR events may explain the basis some proto-oncogene activation cases observed in cancer cells. In this study, we investigated whether 17 β-estradiol (E2), a potent proliferation signal, induces activate proto-oncogenes estrogen receptor...
Abstract The endosomal system is a highly dynamic multifunctional organelle, whose complexity regulated in part by reversible ubiquitylation. Despite the wide-ranging influence of ubiquitin processes, relatively few enzymes utilizing have been described to control endosome integrity and function. Here we reveal deubiquitylating enzyme (DUB) ubiquitin-specific protease 32 (USP32) as powerful player this context. Loss USP32 inhibits late (LE) transport recycling LE cargos, resulting dispersion...
Alternative polyadenylation (APA) plays a role in gene expression regulation generally by shortening of 3'UTRs (untranslated regions) upon proliferative signals and relieving microRNA-mediated repression. Owing to high indices triple negative breast cancers (TNBCs), we hypothesized APA cause 3'UTR length changes this aggressive subgroup cancers. Our probe-based meta-analysis approach identified alterations where the significant majority was events (∼70%, 113 165) mostly proliferation-related...
Mounting evidence suggests involvement of deregulated microRNA (miRNA) expression during the complex events tumorigenesis. Among such miRNAs in cancer, miR-125b is reported to be consistently low breast cancers. In this study, we screened a panel cancer cell lines (BCCLs) for and detected decreased 14 19 BCCLs. Due heterogeneity cancers, MCF7 cells were chosen as model system ERBB2 independent cancers restore (MCF7-125b) investigate phenotypical related functional changes. Earlier, was shown...
Aurora Kinase A (AURKA) is an oncogenic kinase with major roles in mitosis, but also exerts cell cycle- and kinase-independent functions linked to cancer. Therefore, control of its expression, as well activity, crucial. short a long 3'UTR isoform exist for AURKA mRNA, resulting from alternative polyadenylation (APA). We initially observed that triple-negative breast cancer, where typically overexpressed, the predominant this correlates faster relapse times patients. The characterized by...
There is substantial interest in implementing technologies that allow comparisons of whole genomes individuals and tissues cell populations. Restriction landmark genome scanning (RLGS) a highly resolving gel-based technique which several thousand fragments genomic digests are visualized simultaneously quantitatively analyzed. The widespread use RLGS has been hampered by difficulty deriving sequence information for displayed lack whole-genome sequence-based framework interpreting patterns. We...
Abstract Cell division is a highly regulated process. Checkpoints can halt cell‐cycle progression due to adverse conditions such as misalignment of chromosomes prevent missegregation. The search for new regulators the cell cycle revealed mitotic checkpoint gene CHFR (checkpoint with forkhead‐associated and ring finger). coordinates an early phase by delaying chromosome condensation in response stress. Because aneuploidy instability are common malignant breast tumors, we screened 24 cancer...
Cellular differentiation in the gut is vital maintaining cellular and functional specialization of epithelial layer. MicroRNAs (miRNAs) have recently emerged as one key players orchestrating process gut. Using spontaneously differentiating Caco‐2 cell line, we observed an increased expression miR‐146a but not miR‐146b course differentiation. Bioinformatic analyses revealed that membrane type matrix metalloprotease 16 (MMP16, MT3‐MMP) was a predicted target decrease mRNA protein MMP16...
Certain aspects of diagnosis, prognosis, and treatment cancer patients are still important challenges to be addressed. Therefore, we propose a pipeline uncover patterns alternative polyadenylation (APA), hidden complexity in transcriptomes, further accelerate efforts discover novel genes pathways. Here, analyzed expression data for 1045 found significant shift usage poly(A) signals common tumor types (breast, colon, lung, prostate, gastric, ovarian) compared normal tissues. Using...
17823 is a frequent site of gene amplification in breast cancer. Several lines evidence suggest the presence multiple amplicons on 17823. To characterize distinct and localize putative oncogenes, we screened genes expressed sequence tags (ESTs) existing physical radiation hybrid maps for overexpression cancer cell by semiquantitative duplex PCR, RT-PCR, Southern blot, Northern blot analyses. We identified two 17823, one including TBX2 another proximal region RPS6KB1 (PS6K) MUL. In addition...
A new dual channel micro-electrophoresis system for rapid mutation detection based on heteroduplex analysis was designed and implemented. Mutation successfully achieved in a total separation length of 250 microm less than 3 min 590 bp DNA sample harboring causing an amino acid change. Parylene-C used as the structural material fabricating micro-channels it provides conformal deposition, transparency, biocompatibility, low background fluorescence without any surface treatment. architecture...