A5008403593- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Photodynamic Therapy Research Studies
- Colorectal Cancer Treatments and Studies
- Skin Protection and Aging
- Neutropenia and Cancer Infections
- Cell Adhesion Molecules Research
- Streptococcal Infections and Treatments
- Parasites and Host Interactions
- Biosimilars and Bioanalytical Methods
- Probiotics and Fermented Foods
- Immune Response and Inflammation
- melanin and skin pigmentation
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Asthma and respiratory diseases
- Plant-Microbe Interactions and Immunity
- Blood disorders and treatments
- Antimicrobial Resistance in Staphylococcus
- Cancer Immunotherapy and Biomarkers
- Sepsis Diagnosis and Treatment
Massachusetts General Hospital
2019-2024
Boston College
2021
Purpose Coronavirus disease-2019 (COVID-19) is associated with a wide spectrum of clinical symptoms including acute respiratory failure. Biomarkers that can predict outcomes in patients COVID-19 assist patient management. The aim this study to evaluate whether procalcitonin (PCT) outcome and bacterial superinfection infected severe syndrome coronavirus-2 (SARS-CoV-2). Methods Adult diagnosed SARS-CoV-2 by nasopharyngeal PCR who were admitted tertiary care center Boston, MA infection between...
Neutrophils are recognized to represent significant immune cell mediators for the clearance and elimination of human-pathogenic fungal pathogen Candida . The sensing fungi by innate cells is performed, in part, through lectin receptor recognition wall components downstream cellular activation signaling components, including spleen tyrosine kinase (Syk). While essential role Syk macrophages dendritic clear, there remains uncertainty with respect its contribution neutrophils. In this study, we...
Neutrophils are the most abundant white blood cell in body and key participants defense against fungal infections. Fungal infections occur often patients with cirrhosis associated increased 30‐day 90‐day mortality. Previous studies have shown that specific neutrophil functions abnormal cirrhosis, although extent of dysfunction is not well understood. We tested ability neutrophils from 21 hospitalized 23 healthy control to kill Candida albicans , a common pathogen cirrhosis. Using an assay,...
Abstract Background Solid organ transplant (SOT) and stem cell (SCT) recipients are at increased risk of invasive fungal disease despite normal neutrophil counts. Here, we measure anti-Candida activity. Methods Twenty-one SOT 19 SCT were enrolled 2–4 months posttransplant compared to 23 healthy control patients (HC). Neutrophils coincubated with Candida albicans, percentage killing swarming responses measured. Results from had decreased fungicidal capacity HC (42%, 43%, 72% for SCT, SOT, HC,...
The use of mature neutrophil (granulocyte) transfusions for the treatment neutropenic patients with invasive fungal infections (IFIs) has been focus multiple clinical trials. Despite these efforts, transfusion neutrophils resulted in limited benefit, likely owing to problems insufficient numbers and very short lifespan donor cells. In this report, we employed a system conditionally immortalized murine progenitors that are capable continuous expansion, allowing generation unlimited homogenous...
Melanin nanoparticles are known to be biologically benign human cells for a wide range of concentrations in high glucose culture nutrition. Here, we show cytotoxic behavior at nanoparticle and low concentrations, as well concentration under exposure (nonionizing) visible radiation. To study these effects detail, developed highly monodispersed melanin (both uncoated glucose-coated). In order the effect significant cellular uptake nanoparticles, employed three cancer cell lines: VM-M3, A375...
Hyphae formation is a key step for fungal penetration into epithelial cells and escaping from macrophages or neutrophils. We found that 405 nm light-induced catalase deactivation results in the inhibition of hyphae growth Candida albicans. The treatment capable inhibiting across multiple hyphae-producing species. Metabolic studies on light-treated C. albicans reveal light strong reduction both lipid protein metabolism. A significant decrease unsaturated saturated fatty acids was detected...
Necrotizing fasciitis is a potentially fatal soft tissue infection that requires prompt clinical suspicion, pharmacological and surgical interventions. Bacterial pathogens, such as beta-hemolytic streptococcus Staphylococcus aureus, are the main etiology of necrotizing fasciitis, however, rare cases caused by fungal Candida albicans, have been reported following trauma. Here, we present first case C. albicans an elective procedure in immunocompetent adult.
Abstract An impaired neutrophil response to pathogenic fungi puts patients at risk for fungal infections with a high of morbidity and mortality. Acquired dysfunction in the setting iatrogenic immune modulators can include inhibition critical kinases such as spleen tyrosine kinase (Syk). In this study, we used an established system conditionally immortalized mouse progenitors investigate ability augment Syk-deficient function against Candida albicans TLR agonist signaling. LPS, known...
ABSTRACT Neutrophils communicate with one another and amplify their destructive power through swarming, a collective process that synchronizes the activities of multiple neutrophils against target. The sequence contributing to swarming clusters fungi has been recently uncovered. However, molecular signals controlling neutrophils’ during are just emerging. Here, we report spleen tyrosine kinase (SYK) inhibitors severely impair neutrophil responses, resulting in complete loss fungal...
ABSTRACT Once perceived to be homogenous effector cells, neutrophils have since been shown exhibit population heterogeneity. Here, we established an experimental model of clonal neutrophil heterogeneity using conditionally immortalized granulocyte monocyte progenitors (GMPs) and their mature progeny. Transcriptional epigenetic profiling showed conserved genome-wide signatures transcription chromatin accessibility that were specific individual GMP clones paired progeny, suggesting clone...
Abstract Background Solid-organ (SOT) and stem cell transplant (SCT) recipients are at increased risk of invasive fungal disease despite normal neutrophil counts in peripheral blood. However, the neutrophils function against fungi has not been completely defined. In this study, we measure human anti-candida activity SOT SCT recipients. Methods patients were identified consented from September 2018 until April 2019. Healthy control (HC) primary care clinics. EDTA-anticoagulated blood was...