A5019160578- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Cellular transport and secretion
- Sphingolipid Metabolism and Signaling
- Photosynthetic Processes and Mechanisms
- Adenosine and Purinergic Signaling
- Innovative Teaching and Learning Methods
- Endoplasmic Reticulum Stress and Disease
- Education and Critical Thinking Development
- Mitochondrial Function and Pathology
- Technology-Enhanced Education Studies
- Amyotrophic Lateral Sclerosis Research
- Plant Surface Properties and Treatments
- Biochemical Acid Research Studies
- Experimental Learning in Engineering
- Plant Molecular Biology Research
- E-Learning and Knowledge Management
- Educational Innovations and Challenges
- Erythrocyte Function and Pathophysiology
- Muscle Physiology and Disorders
- Alzheimer's disease research and treatments
- Foreign Language Teaching Methods
- Innovative Teaching Methods
University of Calgary
2015-2024
Délégation Provence et Corse
2007
Actively maintained close appositions between organelle membranes, also known as contact sites, enable the efficient transfer of biomolecules cellular compartments. Several such sites have been described well their tethering machineries. Despite these advances we are still far from a comprehensive understanding function and regulation most sites. To systematically characterize site proteomes, established high-throughput screening approach in Saccharomyces cerevisiae based on co-localization...
Phagocytosis of filamentous bacteria occurs through tubular phagocytic cups (tPCs) and takes many minutes to engulf these filaments into phagosomes. Contravening the canonical pathway, tPCs mature by fusing with endosomes. Using this model, we observed sequential recruitment early late endolysosomal markers elongating tPCs. Surprisingly, regulatory endosomal lipid phosphatidylinositol-3-phosphate (PtdIns(3)P) persists on as long their luminal pH remains neutral. Interestingly, manipulating...
Abstract Fundamental changes in the composition and distribution of lipids within brain are believed to contribute cognitive decline associated with Alzheimer’s disease (AD). The mechanisms by which these lipid affect cellular function ultimately cognition not well understood. Although “candidate gene” approaches can provide insight into effects dysregulated metabolism they require a preexisting understanding molecular targets individual species. In this report we combine unbiased gene...
Diacylglycerol (DAG) is a key signaling lipid and intermediate in metabolism. Our knowledge of DAG distribution dynamics cell membranes limited. Using live‐cell fluorescence microscopy we investigated the localization yeast cytosolic‐facing pools response to conditions where homeostasis levels were known be altered. Two main monitored over time using sensors. One pool was associated with vacuolar other localized sites polarized growth. Dynamic changes observed during resumption growth from...
Abstract Actively maintained close appositions, or contact sites, between organelle membranes, enable the efficient transfer of biomolecules various cellular compartments. Several such sites have been described together with their tethering machinery. Despite these advances we are still far from a comprehensive understanding function and regulation most sites. To systematically characterize proteome support discovery new tethers functional molecules, established high throughput screening...
Growth resumption from stationary phase in Saccharomyces cerevisiae, is characterized by lipid droplet (LD) consumption and channeling of precursors toward synthesis membranes. We have previously determined that triacylglycerol lipolysis contributes to a pool diacylglycerol (DAG) associated with the yeast vacuole enriched structures are close proximity LDs. In this study we monitored these using DAG sensor fused GFP during isolation A unique fraction containing sensor, low presence LDs, was...
Diacylglycerol (DAG) was the first lipid second messenger to be discovered in canonical activation pathway of protein kinase C (PKC). The informational outcomes DAG seem intimately connected with its spatial distribution within cell necessitating methods track different cellular pools. We have previously developed a fluorescent probe based on C1 domain PKCδ monitor cytoplasmic facing pools yeast using fluorescence live microscopy. Two were identified both vacuole and at sites polarized...
The synthetic lysophosphatidylcholine (LPC) analogue edelfosine and the naturally occurring platelet activating factor (PAF) are structurally related lipids. Edelfosine is a potent antitumor drug, while PAF neurotoxic lipid that has been implicated in Alzheimer's disease. These LPC analogues also toxic to budding yeast, which can be used as an excellent model system investigate their mode of action. induce alterations membrane organization, metabolism signaling yeast. Phosphatidic acid (PA)...
<p>Phagocytosis of filamentous bacteria occurs through tubular phagocytic cups (tPCs) and takes many minutes to engulf these filaments into phagosomes. Contravening the canonical pathway, tPCs mature by fusing with endosomes. Using this model, we observed sequential recruitment early late endolysosomal markers elongating tPCs. Surprisingly, regulatory endosomal lipid phosphatidylinositol-3-phosphate (PtdIns(3)P) Persists on as long their luminal pH remains neutral. Interestingly,...
<p>Phagocytosis of filamentous bacteria occurs through tubular phagocytic cups (tPCs) and takes many minutes to engulf these filaments into phagosomes. Contravening the canonical pathway, tPCs mature by fusing with endosomes. Using this model, we observed sequential recruitment early late endolysosomal markers elongating tPCs. Surprisingly, regulatory endosomal lipid phosphatidylinositol-3-phosphate (PtdIns(3)P) Persists on as long their luminal pH remains neutral. Interestingly,...
Many diacylglycerol (DAG) sensing probes have been developed to monitor DAG signalling in mammalian cells. Several C1 domains fused fluorescent proteins that can sense cellular membranes. The domain of PKCδ is very well characterized and known bind with high affinity, has successfully used as a probe GFP order live We cloned the (C1δ) into centromeric yeast expression vector under constitutive promoter for vivo visualization pools. Localization C1δ‐GFP was first monitored different wild type...
Mutant superoxide dismutase 1 (mtSOD1) causes dominantly inherited amyotrophic lateral sclerosis (ALS). The mechanism for mtSOD1 toxicity remains unknown. Two main hypotheses are the impairment of proteasomal function and chaperone depletion by misfolded mtSOD1. Here, we employed FRET/FLIM biosensor imaging to quantitatively localize ubiquitination as well binding in single cells, assess their effect on protein folding activities. We found large differences interaction levels wild-type (wt)...
The location of lipids and their distributions across cellular membranes have critical biological consequences, particularly for involved in signaling. In this study, diacylglycerol pools were monitored budding yeast under conditions where lipid homeostasis was altered. Two predominant visualized using the C1 domain mammalian PKCδ fused to GFP. One pool associated with vacuolar other localized sites polarized growth. Upon growth resumption, appeared more dynamic than those phosphatidic acid...
Diacylglycerol (DAG) is a key signaling lipid and intermediate in metabolism. Stress nutritional conditions that induce DAG synthesis also promote triacylglycerol (TAG) production biogenesis of droplets (LDs) budding yeast. Compared to our knowledge on the spatiotemporal details leading channeling into TAG LD emergence, we know significantly less how produced from lipolysis directed towards phospholipids for membrane proliferation. Upon re‐entry growth stationary phase mainly supported by...
Effective oral and written communication skills are key assets for success in outside of academia. However, students completing undergraduate degrees often not equipped with these skills. Among the challenges that contribute to this problem is lack interest by teachers conduct in‐class activities provide opportunities nurture It can be very time consuming a single instructor construct implement multiple active learning activities, designed encourage give chance develop important...