A5040345301- RNA Interference and Gene Delivery
- Advancements in Transdermal Drug Delivery
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Advanced Drug Delivery Systems
- Immune Response and Inflammation
- Retinal Development and Disorders
- Transgenic Plants and Applications
- Surfactants and Colloidal Systems
- Nanoparticle-Based Drug Delivery
- Carbon Nanotubes in Composites
- Dermatology and Skin Diseases
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Nerve injury and regeneration
- Graphene and Nanomaterials Applications
- Toxin Mechanisms and Immunotoxins
- DNA and Nucleic Acid Chemistry
- Nanoparticles: synthesis and applications
- Nanotechnology research and applications
- Glaucoma and retinal disorders
- Microbial Inactivation Methods
University of Waterloo
2016-2025
Andover Eye Associates
2018
Pharmaceutical Biotechnology (Czechia)
2018
Emmanuel Bible College
2018
Regional Municipality of Waterloo
2015
Institute for Biotechnology and Bioengineering
2015
Institute of Nanotechnology
2015
Nebraska Medical Center
2014
University of Saskatchewan
2000-2012
Max Planck Institute for Dynamics and Self-Organization
2008
AbstractThe fate of liposomes and the encapsulated drug was studied after topical application on skin. Lidocaine applied forearm human volunteers produced greater local anaesthetic effect in liposomal form than cream (p≤ 0.001 1h application). Autoradiography demonstrated higher concentration (p≤001) 14C-lidocaine epidermis dermis guinea pigs treated with liposome-encapsulated lidocaine as opposed to Dermabase® cream. Electron microscopic observations, using colloidal iron an electrondense...
The outermost layer of the skin, known as stratum corneum (SC), is composed dead corneocytes embedded in an intercellular lipid matrix consisting ceramides, free fatty acids, and cholesterol. high level organization within this protects body by limiting permeation most compounds through skin. While essential for its protective functions, SC poses a significant barrier delivery topically applied pharmaceutical agents. Chemical enhancers (CPEs) can increase small drug into skin interacting...
Localized scleroderma (morphea and linear scleroderma) is a connective tissue disease, accompanied by excessive proliferation deposition of collagen within the skin, inflammation, vasculopathy deranged immune system. Interferon gamma (IFNgamma), an inhibitor synthesis immunomodulator, could be potential therapeutic agent if it delivered into or expressed locally in affected skin non-invasive manner. In this study, feasibility topical delivery IFNgamma gene expression were investigated...
Abstract Background Increases in DNA transfection efficiencies for non‐viral vectors can be achieved through rational design of novel cationic building blocks. Based on previous results examining condensation by polyamines, gemini surfactants have been designed that incorporate aza or imino substituents within the spacer group order to increase interactions with and potentially improve their ability. Methods Transfection cell toxicity nanoparticles constructed from plasmid DNA, surfactant, a...
Cationic gemini surfactants, N,N-bis(dimethylalkyl)-α,ω-alkanediammonium dibromide [CmH2m+1(CH3)2N+(CH2)sN+(CH3)2CmH2m+1·2Br−, or m-s-m], have proven to be effective synthetic vectors for gene delivery (transfection). Complexes (lipoplexes) of compounds, where m = 12, s 3, 12 and 18 : 1(oleyl), 2, 6, with DNA been investigated using isothermal titration calorimetry (ITC), dynamic light scattering (DLS), zeta potential, atomic force microscopy (AFM) circular dichroism (CD) techniques. The...
Electroporation has been shown to increase the potency of DNA vaccines that have demonstrated significant potential in mice. However, there is a need develop noninvasive or minimally invasive vaccination methods. In pigs, vivo gene expression was assessed compare intradermal needle injection needle-free dermal BioJect as means delivery plasmids. Each administration method further tested with and without surface electroporation. Experiments plasmid encoding luciferase results higher levels...
Background: Carbon nanotubes (CNTs) are novel materials with considerable potential in many areas related to nanomedicine. However, a major limitation the development of CNT-based therapeutic nanomaterials is lack reliable and reproducible data describing their chemical structural composition. Knowledge properties including purity, quality, dispersion state, concentration essential before CNTs see widespread use vitro vivo experiments. In this work, we describe characterization several...
This study aimed to develop a more effective non-viral gene delivery system for keratinocyte transfection. To this end, gemini nanoparticles were formulated from plasmid DNA, the lipid DOPE (dioleoylphosphatidylethanolamine) and surfactants, where surfactant components are novel pH-sensitive derivatives based on m-7-m (alkyl chain–spacer–alkyl chain, m-s-m) unsubstituted base structure. The resultant 1,9-bis(alkyl)-1,1,9,9-tetramethyl-5-amino-1,9-nonanediammonium dibromide m-7NH-m, m = 12,...
Gene based therapy represents an important advance in the treatment of diseases that heretofore have had either no or cure. To capitalize on true potential gene therapy, there is a need to develop better delivery systems can protect these therapeutic biomolecules and deliver them safely target sites. Recently, we designed developed series novel amino acid-substituted gemini surfactants with general chemical formula C12H25(CH3)2N+- (CH2)3-N(AA)-(CH2)3-N+(CH3)2-C12H25 (AA= glycine, lysine,...