A5088935363- Advancements in Transdermal Drug Delivery
- Cancer Cells and Metastasis
- Glioma Diagnosis and Treatment
- Bacillus and Francisella bacterial research
- Synthesis and biological activity
- RNA Interference and Gene Delivery
- Dermatology and Skin Diseases
- Drug Solubulity and Delivery Systems
- Nanoplatforms for cancer theranostics
- Synthesis and Biological Evaluation
- Advanced Drug Delivery Systems
- Microbial Inactivation Methods
- Lipid Membrane Structure and Behavior
- Immunotherapy and Immune Responses
- Contact Dermatitis and Allergies
- Bacterial Genetics and Biotechnology
- Poxvirus research and outbreaks
- Cancer-related Molecular Pathways
- FOXO transcription factor regulation
- Analytical Methods in Pharmaceuticals
- Immune cells in cancer
- 3D Printing in Biomedical Research
- Hedgehog Signaling Pathway Studies
- Monoclonal and Polyclonal Antibodies Research
- Bioactive Compounds and Antitumor Agents
ESIC Hospital
2025
Datta Meghe Institute of Medical Sciences
2025
Manipal Academy of Higher Education
2015-2024
All India Institute of Medical Sciences
2013-2023
All India Institute of Medical Sciences Bhopal
2013-2023
All India Institute of Medical Sciences Raipur
2013-2023
Indira Gandhi Institute of Child Health
2021
Pennsylvania State University
2020
Helix Biopharma (Canada)
2013-2017
Dr. Ram Manohar Lohia Institute of Medical Sciences
2017
Glioblastoma multiforme (GBM) is paradigmatic for the investigation of cancer stem cells (CSC) in solid tumors. Growing evidence suggests that different types CSC lead to formation GBM. This has prompted present comparison gene expression profiles between 17 GBM lines and their putative founder cells. Using a newly derived 24-gene signature, we can now distinguish two subgroups GBM: Type I display "proneural" signature genes resemble fetal neural cell (fNSC) lines, whereas type II show...
Epilepsy affects ∼60 million people worldwide. Most antiseizure medications in the market act on voltage-gated sodium or calcium channels, indirectly modulating neurotransmitter GABA glutamate levels multiple targets. Earlier studies made significant efforts to directly deliver into brain with varied success. Herein, we have hypothesized exogenous epilepsy through extracellular vesicles (EVs) from human GABA-producing cells and their progenitors as EVs largely mimic parent cell composition.
Localized scleroderma (morphea and linear scleroderma) is a connective tissue disease, accompanied by excessive proliferation deposition of collagen within the skin, inflammation, vasculopathy deranged immune system. Interferon gamma (IFNgamma), an inhibitor synthesis immunomodulator, could be potential therapeutic agent if it delivered into or expressed locally in affected skin non-invasive manner. In this study, feasibility topical delivery IFNgamma gene expression were investigated...
Immune cell infiltration varies widely between different glioblastomas (GBMs). The underlying mechanism, however, remains unknown. Here we show that TGF-beta regulates proliferation, migration, and tumorigenicity of mesenchymal GBM cancer stem cells (CSCs) in vivo vitro. In contrast, proneural CSCs resisted due to TGFR2 deficiency. vivo, a substantially increased immune was observed GBMs, while infiltrates were rare GBMs. On functional level, CSC lines caused significantly stronger...
This review outlines the chemistry and anticancer cytotoxic properties of certain Mannich bases. It is divided into four parts. The first section summarizes briefly various investigations undertaken by other researchers chemical pharmacological a number bases against neoplasms. remaining portions this are summaries work in laboratory. second part presents following aspects namely differences antileukemic activity between conjugated styryl ketones related bases, intramolecular cyclization...
Viral infections are the predominant cause of acute hemorrhagic conjunctivitis (AHC) across globe. From July to August 2023, a large-scale outbreak was witnessed in India, affecting large number individuals. The present study initiated identify causative agent responsible for AHC Kalburgi district Karnataka state.
Paracetamol, also known as acetaminophen, is a widely used non-opioid analgesic and antipyretic agent. As an easily accessible over-the-counter (OTC) medication, the risk of exceeding maximum daily dosage resulting in overdose significant. Here, we present case paracetamol 24-year-old male who consumed 15 tablets (9.75 g) given OTC to relieve his chest pain. The patient was treated with N-acetylcysteine (NAC) managed symptomatically. This involves from patient’s inadequate comprehension term...
A novel immunoconjugate (L-DOS47) was developed and characterized as a therapeutic agent for tumors expressing CEACAM6. The single domain antibody AFAIKL2, which targets CEACAM6, expressed in the Escherichia coli BL21 (DE3) pT7-7 system. High purity urease (HPU) extracted purified from Jack bean meal. AFAIKL2 activated using N-succinimidyl [4-iodoacetyl] aminobenzoate (SIAB) cross-linker then conjugated to urease. activation conjugation reactions were controlled by altering pH. Under these...
The current standard therapy for GBM includes maximal surgical resection, followed by concurrent radiotherapy and oral chemotherapy; however, the median post-diagnosis survival period is only 15–18 months. Nanoparticles (NPs) their composite drug delivery systems (DDSs)—particularly those with liposomes polymeric nanoparticles (PNPs)—have become an increasingly popular route therapeutic in glioblastoma multiforme (GBM). These also have added benefit of enabling combinational therapies, which...
Noninvasive transdermal insulin delivery could provide diabetic patients with sustained physiological levels of basal in a pain-free manner. We have developed novel lipid-based system (Biphasix) suitable for macromolecule across the skin. The objective this study was to evaluate pharmacological effects Biphasix-insulin rat model. Transdermal patches (one per animal) containing formulation (10 mg recombinant human dose) were applied shaved abdominal skin streptozotocin-induced rats 48 h....
Noninvasive delivery of macromolecules across intact skin is challenging but would allow for needle-free administration many pharmaceuticals. Biphasic vesicles, a novel lipid-based topical system, have been shown to deliver into the skin. Investigation mechanism interferon alpha (IFN α), as model protein, by biphasic vesicles could improve understanding molecular transport through stratum corneum and design more effective systems. The interaction with human isolated membrane was investigated...
Background and aim: Glioblastoma (GBM) is one of the most common aggressive brain tumors with a median survival 12-14 months. The aim present study was to evaluate gene expression profile stem cell markers Nanog CD24 in GBM determine its relationship outcome terms treatment response overall survival. Material methods: This retrospective as well which included 51 histologically confirmed cases GBM. Expression CD24, evaluated by RT-PCR. Control tissue debrided from open head injury cases. All...
The anthrax edema toxin comprises two proteins: protective antigen and factor. Anthrax binds to the receptors on surface of target cells facilitates entry factor into these cells. Edema (EF) is an adenylate cyclase that catalyzes synthesis cyclic AMP (cAMP) in cytosol host In this study, we examined requirement extracellular calcium for toxin-induced toxicity cAMP response generated by was analyzed a variety cells, including CHO, macrophage-like RAW264.7, human neutrophils, lymphocytes. Our...
Purpose: Topical biphasic vesicle delivery system encapsulating interferon alpha (IFN α) was developed as an alternative to injections used treat human papillomavirus (HPV) infections. Methods: Biphasic lipid vesicles increasing doses of IFN α (biphasic were characterized for encapsulation efficiency, size, zeta potential and structure by centrifugation, dynamic light scattering, confocal microscopy small-angle x-ray scattering. IFNα into skin in vivo topical efficacy patients with genital...
Glioblastoma multiforme (GBM) cancer stem cells (GSCs) are one of the strongest contributing factors to treatment resistance in GBM. Identification biomarkers capable directly affecting these within bulk tumor is a major challenge associated with development new targeting strategies. In this study, we focus on understanding potential multifunctional extraordinaire survivin as biomarker for GSCs. We analyzed expression profiles gene using various publicly available datasets understand its...
The structural gene for anthrax edema factor (EF) was expressed in Escherichia coli under the control of a powerful T5 promoter to yield 89-kDa recombinant protein that reacted with anti-EF antibodies. Recombinant EF purified homogeneity by two-step procedure involving metal chelate affinity chromatography and cation-exchange chromatography. From 1 liter culture, 2.5 mg biologically active easily purified. This is first report purification from E. coli. functionally as its Bacillus anthracis...
Background Small animal models of human diseases are an indispensable aspect pre-clinical research. Being dynamic, most pathologies demand extensive longitudinal monitoring to understand disease mechanisms, drug efficacy and side effects. These considerations often the concomitant development systems with sufficient temporal spatial resolution. Methodology Results This study attempts configure optimize a clinical 3 Tesla magnetic resonance scanner facilitate imaging small central nervous...