Abstract This study demonstrates that IL-23 stimulates the differentiation of human osteoclasts from peripheral blood mononuclear cells (PBMC). Furthermore, in vivo blockade endogenous activity by treatment with anti-IL-23 antibody attenuates collagen-induced arthritis rats preventing both inflammation and bone destruction. induced osteoclastogenesis cultures PBMC absence osteoblasts or exogenous soluble-receptor activator NF-kappaB ligand (RANKL). IL-23-induced was inhibited...

IL-17 is a proinflammatory cytokine crucial for osteoclastic bone resorption in the presence of osteoblasts or synoviocytes rheumatoid arthritis. However, role osteoclastogenesis from human monocytes alone remains unclear. Here, we investigated and direct effect infliximab on induced by IL-17. Human peripheral blood mononuclear cells (PBMC) were cultured 3 days with M-CSF. After non-adherent removed, was added either osteoprotegerin (OPG). Seven later, adherent stained vitronectin receptor....

The current study explored our hypothesis that IFN-gamma-producing human T cells inhibit osteoclast formation. Activated derived from PBMC were divided into (IFN-gamma(+) cells) and IFN-gamma-non-producing (IFN-gamma(-) cells). IFN-gamma(+) cultured with monocytes in the presence of macrophage-CSF alone. concentration soluble receptor activator NF-kappaB ligand (RANKL) IFN-gamma, amount membrane type RANKL expressed on cells, measured by ELISA. In patients early rheumatoid arthritis (RA)...

Objective: To investigate the effectiveness of influenza vaccination in patients with rheumatoid arthritis (RA) from a large practice-based cohort.Method: Patients RA enrolled Institute Rheumatology, Rheumatoid Arthritis (IORRA) cohort completed self-administered questionnaires as part April IORRA surveys 2001, 2002, 2003, and 2007, which included their status occurrence an actual attack. Vaccine coverage rate attack rates were calculated each season. Relative risks (RRs) for evaluated risk...

Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by the destruction of articular cartilage and bone with elevated levels proinflammatory cytokines. It has been reported that IL-17 Th17 cells play important roles in pathogenesis RA. Recently, plasticity helper T demonstrated; can convert to Th1 cells. remains be elucidated whether this conversion occurs early phase Here, we tried identify cells, cell-derived (CD161(+)Th1 cells) peripheral blood early-onset RA...

Objective: Th17 cells, which mainly produce interleukin (IL)-17, have been suggested to play a critical role in the pathogenesis of autoimmune diseases. The plasticity these cells shift Th1 phenotype presence IL-12, has recently reported. However, IL-17 Sjögren's syndrome (SS) and Mikulicz's disease (MD) currently remains unknown. Patients Methods: submandibular salivary gland lymph node MD patient glands 15 SS patients were collected. IFN-γ+ IL-17+ IFN-γ+IL-17+ detected by...

We report a case of Mikulicz's disease, IgG4-related plasmacytic syndrome, successfully treated with corticosteroid and mizoribine, followed by mizoribine alone. A 56-year-old woman experienced persistent bilateral swelling in the eyelids submandibular region accompanied lymphadenopathy without pain. The patient was diagnosed as having disease since her lacrimal glands showed biopsy specimens lymph node diffuse infiltration lymphocytes plasma cells. Symptoms laboratory data were improved...

IL-35 is known as a regulatory cytokine produced by T cells. It has also been reported that suppresses the proliferation of Th17 cells, which involved in pathogenesis many autoimmune diseases. However, rheumatoid arthritis patients, role controversial, and bone metabolism not clarified. We investigated effect on human osteoclast differentiation activation. first evaluated rhIL-35 osteoclastogenesis from monocytes cultured alone, induced soluble-RANKL. examined bone-resorption function mature...

Rheumatoid arthritis (RA) appears as inflammation of synovial tissue and joint destruction. Receptor activator NF- κ B (RANK) is a member the TNF receptor superfamily for RANK ligand (RANKL). In this study, we examined expression of<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msup><mml:mrow><mml:mi mathvariant="normal">R</mml:mi><mml:mi mathvariant="normal">A</mml:mi><mml:mi mathvariant="normal">N</mml:mi><mml:mi...

A teenage girl with systemic lupus erythematosus (SLE) was admitted fever, dry cough, and dyspnea on exertion. Chest computed tomography revealed bilateral diffuse infiltration swelling of the mediastinal lymph nodes. The bronchoalveolar lavage (BAL) fluid light red, suggesting alveolar hemorrhage (DAH). Therefore, glucocorticoid pulse therapy initiated. However, blood BAL cultures showed growth Cryptococcus neoformans. patient diagnosed disseminated cryptococcosis. treated liposomal...

A 34-year-old man who had a history of ulcerative colitis (UC) was admitted to our hospital with complaints arthralgia, erythema nodosum, recurrent oral aphthous ulcers and bloody stools. colonoscopy revealed multiple on his cecum colon also transmural ulcer rectum consistent diagnosis UC. The patient HLA-B51 positive. Based clinical evidence [recurrent ulcers, skin lesions (erythema nodosum), positivity for pathergy test] this diagnosed as having Behçet's disease gastrointestinal...

Rheumatoid arthritis (RA) is a systemic autoimmune disease with chronic joint inflammation characterized by activated T cells. IL-17 and Th17 cells play important roles in the pathogenesis of RA. Recently, plasticity helper has been demonstrated; can convert to Th1 However, it remains be elucidated whether this conversion occurs early phase Here, we validated methods Human Immunology Project using only cell-surface marker through measuring actual expression IFNγ. We also evaluated CD161...

Only a few cases of renal vein thrombosis (RVT) occurring in patients with vasculitis have been reported. RVT associated and hemolytic anemia has not reported yet. We describe here patient complicated by pulmonary embolism, autoimmune anemia, eosinophilic granulomatous polyangiitis. A 69-year-old Japanese man who had treated corticosteroids was referred to our department for severe proteinuria (4.32 g/gCr). Abdominal ultrasonography showed bilateral RVT, contrast-enhanced computed tomography...

Objectives. Drug repositioning or drug reprofiling (DR) has recently been growing in importance. DR a significant advantage over traditional development because the repositioned already passed toxicity tests; its safety is known, and risk of adverse toxicology reduced. In current study, we investigated role rebamipide, mucosa-protecting agent, with reported anti-inflammatory function, human osteoclastogenesis. Methods. Peripheral blood mononuclear cells (PBMCs) were cultured presence M-CSF...

We report a case of Chlamydia-associated arthritis in 40-year-old man. The patient experienced four episodes Chlamydia trachomatis urtethritis within few years. During the present episode, polyarthritis developed days after urethritis was noted. diagnosed as having arthritis. Loxoprofen sodium and azithromycin were started. Antibiotics induced clinical improvement urethritis, although persisted for 3 months. HLA-B27 negative, but both HLA-B35 B40 positive. Thus, we speculate that positivity...

Bucillamine (Bc) is a cysteine derivative with two SH groups, and homolog of d-penicillamine, disease-modifying antirheumatic drug (DMARD) widely used in Japan. However, it remains unclear whether Bc repairs bone erosion patients RA. Here, we treated three RA who subsequently showed radiographic repair erosions cysts.