- Plant tissue culture and regeneration
- Plant and animal studies
- Plant Molecular Biology Research
- Biological and pharmacological studies of plants
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Plant and Fungal Species Descriptions
- Seed Germination and Physiology
- Plant Genetic and Mutation Studies
- Fern and Epiphyte Biology
- Flowering Plant Growth and Cultivation
- Plant Parasitism and Resistance
- Plant biochemistry and biosynthesis
- Ecology and Conservation Studies
- Plant Reproductive Biology
- Plant Diversity and Evolution
- Ecology and Vegetation Dynamics Studies
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Light effects on plants
- Polysaccharides and Plant Cell Walls
- Lignin and Wood Chemistry
- Biochemical and biochemical processes
- Photosynthetic Processes and Mechanisms
- Botany, Ecology, and Taxonomy Studies
Southern Medical University
2024-2025
Nanfang Hospital
2024-2025
South China Botanical Garden
2015-2024
University of Chinese Academy of Sciences
2008-2024
Chinese Academy of Sciences
2015-2024
Beijing Botanical Garden
2014-2023
China National Botanical Garden
2023
Planta
2023
Guangdong Academy of Agricultural Sciences
2012
Kagawa University
2011
This review provides an informative and broad overview of orchid biotechnology, addressing several important aspects such as molecular systematics, modern breeding, in vitro morphogenesis, protoplast culture, flowering control, flower color, somaclonal variation, mycorrhiza, pathogen resistance, virus diagnosis production virus-free plants, functional genomics, genetic transformation, conservation biotechnology pharmaceutical biotechnology. resource will provide valuable insight to...
Paphiopedilum wardii Sumerh. is an endangered tropical terrestrial orchid threatened with extinction due to over-collection and loss of suitable habitats. Asymbiotic germination provides a useful way re-establish plants in the wild (conservation) for commercial propagation. The seeds collected from 180 days after pollination (DAP) were optimum culture vitro, seed reached 65.33% on half-strength MS (1/2 macro- micro-nutrients) medium containing 0.5 mg l−1 α-naphthaleneacetic acid (NAA), 10%...
Abstract Background Cymbidium sinense belongs to the Orchidaceae, which is one of most abundant angiosperm families. C. , a high-grade traditional potted flower, prevalent in China and some Southeast Asian countries. The control flowering time major bottleneck industrialized development . Little known about mechanisms responsible for floral this orchid. Moreover, genome references entire transcriptome sequences do not currently exist Thus, expression profiling data species are needed as an...
The successful ex vitro establishment of Dendrobium plantlets raised in determines the quality end product (cut flowers or potted plants) commercial production for economic gain. When are transplanted from culture room to greenhouse conditions, they may desiccate wilt rapidly and can die as a result changes environment, unless substantial precautions taken adapt new environment. acclimatization vitro-grown an environment by gradually weaning them towards ambient relative humidity light...
Abstract Background The orchids of the subtribe Coelogyninae are among most morphologically diverse and economically important groups within subfamily Epidendroideae. Previous molecular studies have revealed that is an unambiguously monophyletic group. However, intergeneric infrageneric relationships largely unresolved. There has been long controversy over classification genera subtribe. Results complete chloroplast (cp.) genomes 15 species in were newly sequenced assembled. Together with...
Dendrobium officinale is one of the most important Chinese medicinal herbs. Polysaccharides are main active ingredients D. officinale. To identify genes that maybe related to polysaccharides synthesis, two cDNA libraries were prepared from juvenile and adult officinale, named Dendrobium-1 Dendrobium-2, respectively. Illumina sequencing for generated 102 million high quality reads assembled into 93,881 unigenes with an average sequence length 790 base pairs. The Dendrobium-2 86 114,098 695...
Abstract Santalum album L. (Indian sandalwood) is an economically important plant species because of its ability to produce highly valued perfume oils. Little known about the mechanisms by which S. adapts low temperatures. In this study, we obtained 100,445,724 raw reads paired-end sequencing from leaves. Physiological and transcriptomic changes in sandalwood seedlings exposed 4 °C for 0–48 h were characterized. Cold stress induced accumulation malondialdehyde, proline soluble carbohydrates,...
It is well known that aromatic essential oils extracted from the heartwood of Santalum album L. have wide economic value. However, little about role terpenoids in response to various adverse environmental stresses as other plants do form signals during plant-environment interactions.In this study, trace amounts volatiles consisting α-santalene, epi-β-santalene, β-santalene, α-santalol, β-santalol, (E)-α-bergamotene, (E)-β-farnesene and β-bisabolene were found leaves mature S. trees. We...
Dendrobium officinale is a precious traditional Chinese medicinal plant because of its abundant polysaccharides found in stems. We determined the composition water-soluble and starch content D. The extracted polysaccharide was as high 35% (w/w). Analysis monosaccharides showed that were dominated by mannose, to lesser extent glucose, small amount galactose, molar ratio 223:48:1. Although also found, less than 10%. This result indicated major stems non-starch polysaccharides, which might be...
Abstract An efficient regeneration system via shoot organogenesis and somatic embryogenesis from in vitro leaf root explants was established for Scaevola sericea the first time. The highest axillary proliferation coefficient (4.8) obtained on Murashige Skoog (MS) medium supplemented with 1.0 mg/L 6-benzyladenine (BA) 0.1 α -naphthaleneacetic acid (NAA) every 45 days. Young leaves roots, which were used as explants, cultured onto different plant growth regulators. Our results showed that only...
Delayed radiation-induced brain injury (RIBI) characterized by progressive cognitive decline significantly impacts patient outcomes after radiotherapy. The activation of NLRP3 inflammasome within microglia radiation is involved in the progression RIBI mediating inflammatory responses. We have previously shown that sulfonylurea receptor 1-transient potential M4 (SUR1-TRPM4) mediates microglial NLRP3-related inflammation following global ischemia. However, role SUR1-TRPM4 remains unclear....
Renanthera imschootiana Rolfe is an endangered tropical epiphytic orchid that threatened with extinction due to over-collection and the loss of suitable habitats. In vitro propagation a useful way mass produce plants for re-establishment in wild commercial propagation. Seeds collected 150 days after pollination (DAP) were optimum stage culture. Seed germination reached 93.1% on quarter-strength MS (i.e., containing quarter macro- micronutrients) medium 0.5 mg l−1 α-naphthaleneacetic acid...
Abstract This paper documents the key anatomical features during development of P. armeniacum zygotic embryos and their ability to germinate asymbiotically in vitro . study also examines effect media seed pretreatments on germination subsequent seedling growth. Seeds collected from pods 45 days after pollination (DAP) did not while 95 DAP seeds displayed highest percentage (96.2%). Most seedlings (50%) developed stage 5 110 whose compact testa had yet fully formed. Suspensor cells were...
The genus Thuniopsis was recently proposed for a rare orchid species T. cleistogama formerly classified in the Thunia. relationships between and its related genera have not yet been conclusively resolved. Recognition of provides new perspective to illustrate morphological diversity plastome evolution within Coelogyninae. In this study, we sequenced assembled complete chloroplast (cp) genomes three accessions two Thunia alba. A total 135 genes were annotated each cp genome, including 89...
This study successfully established an efficient in vitro propagation system for Paphiopedilum SCBG Huihuang90 via protocorm-like body (PLB) formation from seed-derived calluses, PLB proliferation and differentiation, root induction greenhouse acclimatization. Furthermore, 1/2 Murashige Skoog (MS) + 0.025 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) was suitable the of PLBs, 1/2MS 10% coconut water (CW, v/v) 0.5 g/L activated carbon (AC) differentiation. PLBs at different developmental stages...
The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the transition from vegetative to reproductive phase. To understand molecular mechanism of reproduction, three homologous FT genes were isolated and characterized Cymbidium sinense “Qi Jian Bai Mo”, goeringii ensifolium “Jin Si Ma Wei”. contained 618-bp nucleotides with a 531-bp open reading frame (ORF) encoding 176 amino acids (AAs). Alignment AA sequences revealed that CsFT, CgFT CeFT contain conserved domain, which is...