Histone acetylation is modulated through the action of histone acetyltransferases and deacetylases, which play key roles in regulation eukaryotic gene expression. Previously, we have identified a yeast deacetylase REDUCED POTASSIUM DEPENDENCY3 (RPD3) homolog, HISTONE DEACETYLASE19 (HDA19) (AtRPD3A), Arabidopsis thaliana. Here, report further study expression function HDA19. Analysis plants containing HDA19:beta-glucuronidase fusion revealed that HDA19 was expressed throughout life plant most...

The microRNA miR393 has been shown to play a role in plant development and the stress response by targeting mRNAs that code for auxin receptors Arabidopsis. In this study, we verified two rice receptor gene homologs (OsTIR1 OsAFB2) could be targeted OsmiR393 (Os Oryza sativa). Two new phenotypes (increased tillers early flowering) previously observed (reduced tolerance salt drought hyposensitivity auxin) were OsmiR393-overexpressing plants. demonstrated synthetic auxin-analog treatments....

The molecular mechanism of how the histone deacetylase HDA6 participates in maintaining transposable element (TE) silencing Arabidopsis (Arabidopsis thaliana) is not yet defined. In this study, we show that a subset TEs was transcriptionally reactivated and TE reactivation associated with elevated H3 H4 acetylation as well increased H3K4Me3 H3K4Me2 hda6 mutants. Decreased DNA methylation also detected mutants, suggesting silences by regulating status TEs. Similarly, transcripts some these...

Paphiopedilum wardii Sumerh. is an endangered tropical terrestrial orchid threatened with extinction due to over-collection and loss of suitable habitats. Asymbiotic germination provides a useful way re-establish plants in the wild (conservation) for commercial propagation. The seeds collected from 180 days after pollination (DAP) were optimum culture vitro, seed reached 65.33% on half-strength MS (1/2 macro- micro-nutrients) medium containing 0.5 mg l−1 α-naphthaleneacetic acid (NAA), 10%...

Abstract Background Cymbidium sinense belongs to the Orchidaceae, which is one of most abundant angiosperm families. C. , a high-grade traditional potted flower, prevalent in China and some Southeast Asian countries. The control flowering time major bottleneck industrialized development . Little known about mechanisms responsible for floral this orchid. Moreover, genome references entire transcriptome sequences do not currently exist Thus, expression profiling data species are needed as an...

Abstract Background Histone acetyltransferases (HATs) play an important role in eukaryotic transcription. Eight HATs identified rice (OsHATs) can be organized into four families, namely the CBP (OsHAC701, OsHAC703, and OsHAC704), TAF II 250 (OsHAF701), GNAT (OsHAG702, OsHAG703, OsHAG704), MYST (OsHAM701) families. The biological functions of remain unknown, so a comprehensive protein sequence analysis HAT families was conducted to investigate their potential functions. In addition,...

Histone acetylation is one of the vital reversible modifications chromatin structure that regulates gene expression in eukaryotes. acetyltransferases (HATs) and histone deacetylases (HDACs) maintain homeostasis acetylation. Studies Arabidopsis have revealed HATs are involved plant responses to various stresses including light, temperature, salt ABA. Drought stress, a very common environmental could cause range physiological biochemical plants involving HATs. Eight four different families...

s: The histone deacetylases play important roles in the regulation of gene expression and subsequent control a number biological processes, including those involved response to environmental stress. A specific group deacetylase genes, HD2, is present plants. In Arabidopsis, HD2s include HD2A, HD2B, HD2C HD2D. Previous research showed that HD2B are more related terms function, but not this report, we studied different aspects AtHD2D Arabidopsis with respect plant drought other abiotic...

Abstract Plants have evolved to express some members of the nitrate transporter 1/peptide family ( NPF ) uptake and transport nitrate. However, little is known physiological functional roles this in rice Oryza sativa L.). Here, we characterized vascular specific OsNPF2.2. Functional analysis using cDNA-injected Xenopus laevis oocytes revealed that OsNPF2.2 a low-affinity, pH-dependent transporter. Use green fluorescent protein tagged showed located plasma membrane protoplast. Expression...

Light is a major external factor in regulating seed germination. Photoreceptor phytochrome B (PHYB) plays predominant role promoting germination the initial phase after imbibition, partially by repressing phytochrome-interacting factor1 (PIF1). However, mechanism underlying PHYB-PIF1-mediated transcription regulation remains largely unclear. Here, we identified that histone deacetylase15 (HDA15) negative component of PHYB-dependent Overexpression HDA15 Arabidopsis inhibits germination,...

Terpene synthase (TPS) is a critical enzyme responsible for the biosynthesis of terpenes, which possess diverse roles in plant growth and development. Although many terpenes have been reported orchids, limited information available regarding genome-wide identification characterization TPS family orchid, Dendrobium officinale. By integrating D. officinale genome transcriptional data, 34 genes were found These divided into four subfamilies (TPS-a, TPS-b, TPS-c, TPS-e/f). Distinct tempospatial...

Abstract Oolong tea is a semi-fermented Chinese traditional that dates back centuries and now its unique characteristics are attracting more consumers worldwide. The formation of tea's special quality attributed to the proper processing fresh leaf. can be evaluated by aroma, flavor, color, appearance with aroma flavor being two most important indices. distinct depends largely on decomposition lipids carotenoids. However, other compounds hydrolyzed and/or oxidized also contribute aroma....

With the wide use of double-stranded RNA interference (RNAi) for analysis gene function in plants, a high-throughput system making hairpin (hpRNA) constructs is great demand. Here, we describe novel restriction-ligation approach that provides simple but efficient construction intron-containing hpRNA (ihpRNA) vectors. The takes advantage type IIs restriction enzyme BsaI and our new plant RNAi vector pRNAi-GG based on Golden Gate (GG) cloning. This method requires only single PCR product...

Dendrobium officinale is one of the most important Chinese medicinal herbs. Polysaccharides are main active ingredients D. officinale. To identify genes that maybe related to polysaccharides synthesis, two cDNA libraries were prepared from juvenile and adult officinale, named Dendrobium-1 Dendrobium-2, respectively. Illumina sequencing for generated 102 million high quality reads assembled into 93,881 unigenes with an average sequence length 790 base pairs. The Dendrobium-2 86 114,098 695...

Abstract Santalum album L. (Indian sandalwood) is an economically important plant species because of its ability to produce highly valued perfume oils. Little known about the mechanisms by which S. adapts low temperatures. In this study, we obtained 100,445,724 raw reads paired-end sequencing from leaves. Physiological and transcriptomic changes in sandalwood seedlings exposed 4 °C for 0–48 h were characterized. Cold stress induced accumulation malondialdehyde, proline soluble carbohydrates,...

Dendrobium officinale Kimura et Migo is a precious traditional Chinese medicine. Despite D. displaying good salt-tolerance level, the yield and growth of were impaired drastically by increasing soil secondary salinization. The molecular mechanisms plants’ adaptation to salt stress are not well documented. Therefore, in present study, plants treated with 250 mM NaCl. Transcriptome analysis showed that significantly altered various metabolic pathways, including phenylalanine metabolism,...

Reversible histone acetylation and deacetylation at the N-terminus of tails play a crucial role in regulating eukaryotic gene activity. Acetylation core histones is associated with activation, whereas often correlated repression. The level antagonistically catalyzed by acetyltransferases (HATs) deacetylases (HDACs). In this work, we examined subcellular localization, expression pattern function HDT701, member plant-specific HD2-type deacetylase rice. HDT701 localized nucleus. Histochemical...

Santalum album (sandalwood) is one of the economically important plant species in Santalaceae for its production highly valued perfume oils. Sandalwood also a hemiparasitic tree that obtains some water and simple nutrients by tapping into other plants through haustoria which are specialized organs parasitic angiosperms. However, an understanding molecular mechanisms involved haustorium development limited. In this study, RNA sequencing (RNA-seq) analyses were performed to identify changes...

Dendrobium officinale is a precious traditional Chinese medicinal plant because of its abundant polysaccharides found in stems. We determined the composition water-soluble and starch content D. The extracted polysaccharide was as high 35% (w/w). Analysis monosaccharides showed that were dominated by mannose, to lesser extent glucose, small amount galactose, molar ratio 223:48:1. Although also found, less than 10%. This result indicated major stems non-starch polysaccharides, which might be...