This paper summarises the current knowledge concerning cryptic species of plant-parasitic nematode and briefly reviews different methods available for their detection characterisation. Cryptic represent an important component biodiversity, such speciation being common among nematodes occurring in diverse groups with life history traits, including spiral, virus vector, root-lesion false root-knot nematodes. are a number reasons, food security, quarantine, non-chemical management technologies...

The genus Longidorus includes a remarkable group of invertebrate animals the phylum Nematoda comprising polyphagous root-ectoparasites numerous plants including several agricultural crops and trees. Damage is caused by direct feeding on root cells as well transmitting nepoviruses that cause disease those crops. Thus, correct identification species essential to establish appropriate control measures. We provide first detailed information diversity distribution infesting wild cultivated olive...

The phylum Nematoda includes the genus Longidorus, a remarkable group of invertebrates that are polyphagous root-ectoparasites many plants including various agricultural crops and trees. Damage is caused by direct feeding on root cells as well transmitting nepoviruses. Species discrimination in Longidorus complicated phenotypic plasticity (intraspecific variability minor interspecific differences) leading to potential misidentification. We conducted nematode surveys cultivated natural...

The Xiphinema americanum-group constitutes a complex of about 55 species polyphagous plant-ectoparasitic nematodes with worldwide distribution. This group plant-parasitic is one the most difficult dagger nematode complexes for diagnosis because morphology very conservative and morphometric characters often overlap. We conducted surveys in cultivated wild olives southern Spain from 2012 to 2014, which we identified 16 populations X. americanum-group, five were tentatively as belonging three...

Gutiérrez-Gutiérrez, C., Palomares-Rius, J.E., Cantalapiedra-Navarrete, Landa, B.B., Esmenjaud, D. & Castillo, P. (2010). Molecular analysis and comparative morphology to resolve a complex of cryptic Xiphinema species. —Zoologica Scripta, 39, 483–498. During nematode surveys in cultivated natural environments southern Spain nine populations parthenogenic species tentatively identified as cf. pyrenaicum one population morphologically close turcicum were detected. Surveys France also...

Root-knot nematodes of the genus Meloidogyne are recognised worldwide as a major production constraint in crops primary economic importance. Knowledge their diversity and prevalence, well environmental agronomical cues for understanding distribution specific areas is vital importance designing control measures to reduce significant damage. We provide first detailed information on diversity, levels species infecting wild cultivated olive soils wide-region southern Spain that included 499...

Abstract Background Entomopathogenic nematodes (EPNs) represent a guild of soil-inhabiting that rapidly infect and kill insects live in, on or near the ground surface. They are considered an attractive emerging alternative to chemical insecticides, particularly against soil-borne pests like Ceratitis capitata Wiedemann (Diptera: Tephritidae). The virulence different EPN strains from same species can vary according their geographical origin. For this reason, exploring EPNs testing...

The genus Xiphinema constitutes a large group of about 260 species plant-ectoparasitic nematodes. is polyphagous and distributed almost worldwide. Some the this damage agricultural crops by direct feeding on root cells as well transmitting nepoviruses. Species discrimination in complicated phenotypic plasticity leading to potential misidentification. We conducted nematode surveys cultivated natural environments Spain from 2009 2012, which we identified 20 populations morphologically close...

Stunt nematodes are characterized by phenotypic plasticity, with overlapping morphology and morphometry leading to potential misidentification. Consequently, the application of integrative taxonomic approaches is useful species delimitation based on a combination different perspectives, e.g. DNA sequences. We conducted nematode surveys in cultivated natural environments Spain USA, from which we identified 18 known family Telotylenchidae two new taxa within studied samples. These were...

The traditional identification of plant-parasitic nematode species by morphology and morphometric studies is very difficult because high morphological variability that can lead to considerable overlap many characteristics their ambiguous interpretation. For this reason, it essential implement approaches ensure accurate identification. DNA barcoding aids in advances discovery. This study sought unravel the use mitochondrial marker cytochrome c oxidase subunit 1 (coxI) as barcode for...

The dagger nematodes Xiphinema pachtaicum and X. index are two of the most widespread frequently occurring spp. co-infesting vineyards other crops natural habitats worldwide. Sexual reproduction is rare in these species. primary objective this study was to determine genetic structure populations using eight seven populations, respectively, from different "wine denomination origin (D.O.) zones" Spain Sardinia (Italy), by studying mitochondrial (cytochrome oxidase c subunit 1 or COI) nuclear...

Abstract Morphological and molecular analyses of a stem nematode causing severe disease on infected sowthistle ( Sonchus bulbosus ) plants, involving the formation gall-like structures leaves stems, have led to description new species named Ditylenchus oncogenus n. sp. Morphologically, is characterized by medium large body size (all adults more than 1 mm in length); delicate stylet (9.0–11.0 μm long) with minute, rounded knobs; long post-vulval uterine sac c . 65% vulva–anus distance); six...

Longidorid nematodes comprise more than 500 species, and Longidorus Xiphinema are the most diversified, prevalent, cosmopolitan genera within plant-parasitic nematodes. This increases risk of species misidentification. We conducted an integrative morphometric genetic study on two longidorid to elucidate existence new cases cryptic speciation Xiphinema. Detailed morphological, morphometrical, multivariate, studies were carried out, mitochondrial nuclear haploweb analyses used differentiate L....

Bacterial endosymbionts have been detected in some groups of plant-parasitic nematodes, but few cases reported compared to other the phylum Nematoda, such as animal-parasitic or free-living nematodes. This study was performed on a wide variety nematode families and species from different host plants populations. A total 124 populations (previously identified morphologically molecularly) were screened for presence potential bacterial using partial 16S rRNA gene fluorescence situ hybridization...

The genus Xiphinema includes a remarkable group of invertebrates the phylum Nematoda comprising ectoparasitic animals many wild and cultivated plants. Damage is caused by direct feeding on root cells vectoring nepoviruses that cause diseases several crops. Precise identification species critical for launching appropriate control measures. We make available first detailed information diversity distribution infesting olive in wide-region southern Spain included 211 locations from which 453...

The genus Longidorus constitutes a large group of approximately 170 species plant-ectoparasitic nematodes that are polyphagous and distributed almost worldwide. Some the this vectors plant viruses. Species discrimination in is difficult because morphology very conservative, morphometric characters often overlap, leading to potential misidentification. Integrative taxonomy, based on combination molecular analyses with morphology, useful necessary approach identification. In Spain from 2014...

Summary The present study is part of a survey for the identification plant-parasitic nematodes in rhizosphere cultivated and wild olive trees Crete, Greece. Sixteen species corresponding to 13 genera are added 20 belonging 8 genera, previously reported survey. Seven nematode species, Filenchus ditissimus , vulgaris Ogma civellae Pratylenchoides crenicauda Psilenchus hilarulus Tylenchus elegans Zygotylenchus guevarai recorded first time

This study delves into the diagnosis of pin nematodes (Paratylenchus spp.) in Spain based on integrative taxonomical approaches using 24 isolates from diverse natural and cultivated environments. Eighteen species were identified females, males (when available) juveniles with detailed morphology-morphometry molecular markers (D2-D3, ITS COI). Molecular obtained same individuals used for morphological morphometric analyses. The cryptic diversity an approach Paratylenchus straeleni-species...