A5103273647- Ferroelectric and Piezoelectric Materials
- Microbial Metabolites in Food Biotechnology
- Microwave Dielectric Ceramics Synthesis
- Gut microbiota and health
- Probiotics and Fermented Foods
- Plant tissue culture and regeneration
- Marine and coastal plant biology
- Plant Reproductive Biology
- Multiferroics and related materials
- Photosynthetic Processes and Mechanisms
- Microbial Natural Products and Biosynthesis
- Enzyme Catalysis and Immobilization
- Avian ecology and behavior
- Enzyme Production and Characterization
- Plant Parasitism and Resistance
- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Bacterial biofilms and quorum sensing
- Steroid Chemistry and Biochemistry
- Fungal Biology and Applications
- Biofuel production and bioconversion
- Pharmacogenetics and Drug Metabolism
- Biocrusts and Microbial Ecology
- Light effects on plants
- Antimicrobial Resistance in Staphylococcus
Fujita Health University
2023-2025
Fujita Health University Hospital
2024
Japan Food Research Laboratories
2018-2023
Toyama Prefectural University
1999-2022
Institute of Food Science & Technology
2022
Sapporo Science Center
2011
The University of Tokyo
1955-2009
Nutrasource
2004-2007
Kanagawa Prefectural Museum of Natural History
2007
Toyo University
1997-2006
Journal Article Presence of a Na+-activated ATPase in the Plasma Membrane Marine Raphidophycean Heterosigma akashiwo Get access Masato Wada, Wada 1The University Tsukuba, Institute Biological SciencesTsukuba City, Ibaraki, 305 Japan Search for other works by this author on: Oxford Academic Google Scholar Shinobu Satoh, Satoh Kunihiro Kasamo, Kasamo 2Department Cell Biology, National Agrobiological ResourcesTsukuba Tadashi Fujii Plant and Physiology, Volume 30, Issue 6, September 1989, Pages...
Butyrate produced by gut microbiota has multiple beneficial effects on host health, and oligosaccharides derived from diets glycans originating mucus are major sources of its production. A significant reduction butyrate-producing bacteria been reported in patients with inflammatory bowel diseases colorectal cancers. Although butyrate levels important for oligosaccharide metabolic properties producers poorly characterized. We studied the fructooligosaccharides (FOSs) other prebiotic (i.e....
Despite the well-known potential health benefits of prebiotics and non-viable probiotics (paraprobiotics) in various animal species, research regarding their use penguins is scarce. Our study aimed to investigate impact a combined administration paraprobiotics (referred here as "parasynbiotics") on gut microbiome overall Magellanic (Spheniscus magellanicus). The parasynbiotics consisted 1-kestose, which fructooligosaccharide comprising sucrose fructose, heat-killed Lactiplantibacillus...
Feline atopic skin syndrome (FASS) is a chronic inflammatory disease characterized by itching and dermatitis. While the relationship between gut microbiota dermatitis (AD) has been highlighted, exploring gut-targeted therapies for FASS remain limited. This study aimed to evaluate effects of parasynbiotic containing 1-kestose heat-killed Lactobacillus plantarum FM8 on clinical symptoms in cats with FASS. Eleven were treated (400 mg/day) (2.0 × 1010 CFU/day) 8 weeks. Clinical assessed using...
A constitutively produced beta-lactamase was purified from Flavobacterium odoratum GN14053. The enzyme gave a single protein band on polyacrylamide gel electrophoresis. isoelectric point 5.8, and the molecular weight estimated to be about 26,000. activity inhibited by EDTA, iodine, p-chloromercuribenzoate, HgCl2, CuSO4 but not clavulanic acid, sulbactam, imipenem, cephamycin derivatives. showed broad substrate profile, hydrolyzing oxyiminocephalosporins, cephamycins, some penicillins.
1 In 4-day-old etiolated rice seedlings, 3 mm of the coleoptile tip did mainly perceive photostimulus to cause phytochrome-dependent inhibition elongation. At this age, cell elongation occurred most in middle portion coleoptiles dark, and was reversibly controlled by a brief exposure red far-red light. Thus, photoperceptive site evidently separated from growing zone intact coleoptiles. 2 The red-light-induced nullified removal followed exogenous application IAA. sensitivity thus treated IAA...
Abstract We demonstrated high free dielectric permittivity ( ε 33 T / 0 ) > 8000 and piezoelectric coefficient d 1950 pC/N of 72Pb(Mg 1/3 Nb 2/3 )O 3 -28PbTiO (PMN–28PT) single crystals (SCs) obtained by low-voltage AC poling (LV ACP) 1.5 to 3.5 kV RMS cm −1 at a temperature (HT) 80 °C in an air atmosphere. The minimum standard deviation σ 170 was similar conventional DC (DCP) average 8800 confirmed with 2.0 k V ACP for five samples. voltages were less than 50% compared previous studies...
The enzyme involved in the reduction of Δ1-piperideine-6-carboxylate (P6C) to L-pipecolic acid (L-PA) has never been identified. We found that Escherichia coli JM109 transformed with lat gene encoding L-lysine 6-aminotransferase (LAT) converted (L-Lys) L-PA. This suggested there is a "P6C reductase" catalyzes P6C L-PA genome E. coli. complementation experiment proC32 RK4904 for production clearly shows expression both and proC essential biotransformation L-Lys Further, showed LAT...
Background and Aim Mucopolysaccharidosis IVA (MPS IVA) leads to skeletal dysplasia through excessive storage of chondroitin-6-sulfate keratan sulfate (KS). KS is synthesized mainly in cartilage released into circulation, making it a critical|biomarker for MPS evaluate clinical course effectiveness therapies. Therefore, an accurate sensitive method required measure levels. Material Methods Using sandwich ELISA liquid chromatography tandem mass spectrometry (LC/MS/MS) assays, we measured...
It has become clear that the intestinal microbiota plays a role in food allergies. The objective of this study was to assess allergy-preventive effects combined intake short fructan (1-kestose [Kes]) and long (inulin ([Inu]) an ovalbumin (OVA)-induced allergy mouse model.Oral administration fructans lowered allergenic symptom score alleviated decreases rectal temperature total IgA levels increases OVA-specific IgE induced by high-dose OVA challenge, particular, Kes Inu significantly...
Our biotransformation using Escherichia coli expressing a cytochrome P450 (CYP) belonging to the CYP153A family from Acinetobacter sp. OC4 produced great amount of 1-octanol (2,250 mg per liter) n-octane after 24 h incubation. This level production is equivalent maximum previously achieved in experiments alkanes. In addition, initial rate was maintained throughout entire incubation period. These results indicate that we have functional and stable expression CYP E. for first time. Further,...
Tempeh is a traditional Indonesian soybean-fermented food produced by filamentous fungi, Rhizopus sp. and Fusarium We isolated sequenced the genomic gene cDNA clone encoding novel protease (FP) from BLB. The was 856 bp in length contained two introns. An encoded protein of 250 amino acids. predicted acid sequence FP showed highest homology, 76%, with that trypsin oxysporum. hydrolysis activity toward synthetic peptide higher than any other tested, including Nattokinases. Furthermore,...
Butyrate producing bacteria are one of the major components human gut microbiota. Their metabolite, butyrate, has several beneficial properties for host health. Fructooligosaccharides (FOSs) well documented prebiotics and hydrolyzed by intracellular glycoside hydrolase family 32 (GH32) enzyme in butyrate producers, whereas producers Anaerostipes hadrus butyraticus possess extracellular GH32 enzymes. The present study characterized enzymes organisms to consider possible cross-feeding FOSs...
Colorectal cancer (CRC) is a leading cause of deaths, closely linked to the intestinal microbiota and bile acid metabolism. Secondary acids, like deoxycholic lithocholic acid, are associated with increased CRC risk due their disruption vital cellular functions. In contrast, isoallolithocholic (isoalloLCA) shows potential health benefits, highlighting complex role acids in CRC. A specific primer set was previously developed amplify homologs 5α-reductase gene (
(1) Background: Proglucagon-derived peptides (PDGPs) including glucagon (Gcg), GLP-1, and GLP-2 regulate lipid metabolism in the liver, adipocytes, intestine. However, mechanism by which PGDPs participate alterations induced high-fat diet (HFD) feeding has not been elucidated. (2) Methods: Mice deficient PGDP (GCGKO) control mice were fed HFD for 7 days analyzed, differences adipose tissue, duodenum investigated. (3) Results: GCGKO under showed lower expression levels of genes involved free...
Introduction. Hepatocellular carcinoma (HCC) is one of the deadliest cancers worldwide. Gap statement. Monitoring HCC and predicting its immunotherapy responses are challenging. Aim. This study explored potential gut microbiome for monitoring responses. Methods. DNA samples were collected from faeces 22 patients with treated atezolizumab/bevacizumab (Atz/Bev) 85 healthy controls. The was analysed using 16S rRNA next-generation sequencing quantitative PCR (qPCR). Results. microbiomes...
Biotransformation using alkane-oxidizing bacteria or their alkane hydroxylase (AH) systems have been little studied at the molecular level. We cloned and sequenced genes from Gordonia sp. TF6 encoding an AH system, alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 rubB (rubredoxin reductase). When expressed in Escherichia coli, these allowed construction of biotransformation for various alkanes. Normal alkanes with 5 to 13 carbons were good substrates this biotransformation, oxidized...
For the Japan Atomic Energy Agency sodium-cooled fast reactor, an experimental study on fluctuating pressure of hot legs was carried out with tests in a 1/3-scale model. The total resistance coefficient is consistent published data, and, additionally, our research has given data up to Reynolds number 8.0×106. flow visualization and velocity measurement confirmed independence number. Pressures pipe wall were statistically examined predict characteristics pressures legs. It reveals that...
We report here some efficient biotransformations using Escherichia coli strains with disruptions for the AcrAB-TolC efflux pump system. Biotransformations of compactin into pravastatin (6alpha-hydroxy-iso-compactin) were performed E. tolC and/or acrAB mutations expressing a cytochrome P450 (P450) gene. The production levels acrAB, tolC, and increased by 3.7-, 7.0-, 7.1-fold, respectively. Likewise, 25-hydroxy vitamin D3 4-cholesten 3-one mutant an individual gene 2.2- 16-fold, enhancement...