A5072457786- Yersinia bacterium, plague, ectoparasites research
- Zoonotic diseases and public health
- Salmonella and Campylobacter epidemiology
- Bacillus and Francisella bacterial research
- Vector-borne infectious diseases
- Animal Disease Management and Epidemiology
- Identification and Quantification in Food
- Clostridium difficile and Clostridium perfringens research
- Pharmacological Effects of Natural Compounds
- Microbial infections and disease research
- Meat and Animal Product Quality
- Food Safety and Hygiene
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Leptospirosis research and findings
- Animal Behavior and Welfare Studies
- Bee Products Chemical Analysis
- Plant-based Medicinal Research
- Listeria monocytogenes in Food Safety
- Food Supply Chain Traceability
- Vibrio bacteria research studies
- Research in Social Sciences
- Viral Infections and Vectors
- Plant and fungal interactions
- Bacterial Identification and Susceptibility Testing
University of Helsinki
2016-2025
Finnish Food Authority
2018-2021
Ministry of Economic Affairs and Employment
2012
Ministère de la Santé et de l'Hygiène Publique
2012
Significance Our past understanding of pathogen evolution has been fragmented because tendencies to study human clinical isolates. To understand the evolutionary trends pathogenic bacteria though, we need context their nonpathogenic relatives. unique and detailed dataset allows description parallel two key pathogens: causative agents plague Yersinia diarrhea. The analysis reveals an emerging pattern where few virulence-related functions are found in all lineages, representing “foothold”...
Abstract In response to the EFSA call New approaches in identifying and characterizing microbial chemical hazards, project INNUENDO (https://sites.google.com/site/theinnuendoproject/) aimed design an analytical platform standard procedures for use of whole-genome sequencing surveillance outbreak investigation food-borne pathogens. The firstly attempted identify existing flaws needs, then provide applicable cross-sectorial solutions. focused developing a small countries with limited...
Multilocus sequence analysis of 417 strains Yersinia pseudotuberculosis revealed that it is a complex four populations, three which have been previously assigned species status [Y. sensu stricto (s.s.), pestis and similis] fourth population, we refer to as the Korean group, may be in process speciation. We detected clear signs recombination within Y. s.s. well imports from similis group. The sources genetic diversification were approximately equally divided between mutation, whereas has not...
Pigs are considered as a major reservoir of human pathogenic Yersinia enterocolitica and source yersiniosis. However, the transmission route Y. from farm to pork is still unclear. The pigs carcasses pluck sets was investigated by collecting samples 364 individual ear-tagged on at slaughterhouse. In addition, isolated strains were analyzed, using pulsed-field gel electrophoresis. Isolation similar genotypes 4/O:3 in animals slaughterhouse shows that carcass contamination originates pig...
The transmission of Yersinia pseudotuberculosis in the pork production chain was followed from farm to slaughterhouse by studying same 364 pigs different systems at and levels. In all, 1,785 samples were collected, isolated Y. strains analyzed pulsed-field gel electrophoresis. results microbial sampling combined with data an on-farm observation questionnaire study elucidate associations between factors prevalence pseudotuberculosis. Following slaughterhouse, we able show similar genotypes...
The aim of this study was to evaluate the efficiency four isolation methods for detection pathogenic Yersinia enterocolitica from pig intestinal content.The comprised 15 steps using selective enrichments (irgasan-ticarcillin-potassium chlorate and modified Rappaport broth) mildly at 4 or 25 degrees C. Salmonella-Shigella-desoxycholate-calcium chloride agar, cefsulodin-irgasan-novobiocin agar were used as plating media. most sensitive method detected 78% (53/68) positive samples. Individual...
A multiplex real‐time polymerase chain reaction (PCR) assay was developed for the detection of Yersinia pestis and pseudotuberculosis . The includes four primer pairs, two which are specific Y. , one bacteriophage λ; latter used as an internal amplification control. ‐specific target genes in were ypo2088 a gene coding putative methyltransferase, pla plasminogen activator. In addition, wzz to specifically identify both closely related group. probe sets described different can be either single...
To study the origin and spread of Yersinia enterocolitica among pigs, fecal blood samples were repeatedly taken on a fattening farm. A few piglets found to be already infected breeding farms. After mixed, infection through whole unit. Eventually, all pigs excreted pathogen.
SUMMARY A survey of 788 pigs from 120 farms was conducted to determine the within-farm prevalence pathogenic Yersinia enterocolitica and a questionnaire management conditions mailed afterwards. univariate statistical analysis with carriage shedding as outcomes random-effects logistic regression farm clustering factor. Variables P value <0·15 were included into respective multivariate model. The use municipal water discovered be protective factor against faecal pathogen. Organic production...
Abstract Enteropathogenic Yersinia comprising pathogenic enterocolitica and pseudotuberculosis , are zoonotic pathogens causing foodborne intestinal illness in humans. Y. is common pork production associated with infections Therefore, there a need to reduce the occurrence spread of these within chain. It would be most effective control enteropathogenic at farm level. However, present, feasible intervention methods lacking more research needed. The way prevent buy piglets from enterocolitica‐...
ABSTRACT Sporadic and epidemiologically linked Yersinia enterocolitica strains ( n = 379) isolated from fecal samples human patients, tonsil or pigs collected at slaughterhouses, pork meat stores were genotyped using multiple-locus variable-number tandem-repeat analysis (MLVA) with six loci, i.e., V2A, V4, V5, V6, V7, V9. In total, 312 different MLVA types found. Similar detected (i) in patients over 2 to 3 consecutive years, (ii) humans pigs, (iii) that originated the same farms. Among...
Yersinia enterocolitica and pseudotuberculosis are important zoonotic bacteria causing human enteric yersiniosis commonly reported in Europe. All Y. strains considered pathogenic, while include both pathogenic nonpathogenic which can be divided into six biotypes (1A, 1B, 2-5) about 30 serotypes. The most common types Europe bioserotypes 4/O:3 2/O:9. Strains belonging to biotype 1A as because they missing virulence genes like the attachment-invasion-locus (ail) gene chromosome plasmid. role...
Hunting is currently a very popular activity, and interest in game meat increasing. However, only limited research available on the bacterial quality safety of moose (Alces alces) white-tailed deer (Odocoileus virginianus) harvested by hunters. Poor hunting hygiene can spread bacteria onto carcasses, inadequate chilling carcasses may increase load carcass surface. We studied contamination level 100 shot southern Finland. Hunters eviscerated field skinned them small slaughter facilities....
The overall objectives of meat inspection are to contribute food safety, animal welfare, and health. In the European Union (EU), there is a request for modernised system that addresses these in more valid, feasible cost-effective way than does traditional system. One part modernisation deals with coding register findings. Although unified standards set at EU level judgement criteria regarding fitness consumption, different national systems force. question extent differences whether basis...
Campylobacter is an important foodborne pathogen as it associated with significant disease burden across Europe. Among various sources, infections in humans are often related to the consumption of undercooked poultry meat or improper handling meat. Many European countries have implemented measures reduce human exposure from broiler In this paper, surveillance programs some summarized. Our findings reveal that many test neck skin samples for per Process Hygiene Criteria (PHC) set by...
Abstract The post-mortem inspection of domestic pigs within the European Union was revised in 2014, primarily to include visual meat each carcase and offal. Palpations incisions were removed from routine procedures, as they are mostly used detect pathological lesions caused by organisms irrelevant for public health, instead can cause cross-contamination carcases with foodborne pathogens. However, examination all external surfaces organs, declaration patho-physiological unfit human...