Rafiqul Hyder

ORCID: 0000-0002-1554-8840
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About
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Research Areas
  • Plant and Fungal Interactions Research
  • Plant Virus Research Studies
  • SARS-CoV-2 detection and testing
  • Plant Disease Resistance and Genetics
  • Biosensors and Analytical Detection
  • Advanced biosensing and bioanalysis techniques
  • Viral gastroenteritis research and epidemiology
  • Forest Insect Ecology and Management
  • Respiratory viral infections research
  • Antibiotic Resistance in Bacteria
  • SARS-CoV-2 and COVID-19 Research
  • Image Processing and 3D Reconstruction
  • Bacterial Identification and Susceptibility Testing
  • Antibiotic Use and Resistance
  • Pharmaceutical and Antibiotic Environmental Impacts
  • Historical and Archaeological Studies
  • Microbial Community Ecology and Physiology
  • Marine and coastal ecosystems
  • Aquatic Ecosystems and Phytoplankton Dynamics
  • Antibiotics Pharmacokinetics and Efficacy
  • Research in Social Sciences
  • Mycorrhizal Fungi and Plant Interactions

Tampere University
2023-2025

Natural Resources Institute Finland
2017-2021

Finnish Environment Institute
2011

University of Helsinki
2006

Around the world, influenza A virus has caused severe pandemics, and risk of future pandemics remains high. Currently, surveillance is based on clinical diagnosis reporting disease cases. In this study, we apply wastewater-based to monitor amount RNA at population level. We report levels in 10 wastewater treatment plant catchment areas covering 40% Finnish population. Altogether, 251 monthly composite influent samples (collected between February 2021 2023) were analysed from supernatant...

10.1016/j.watres.2024.121650 article EN cc-by Water Research 2024-04-21

Wastewater comprises multiple pathogens and offers a potential for wastewater-based surveillance (WBS) to track the prevalence of communicable diseases. The Finnish WastPan project aimed establish pandemic preparedness (viruses, bacteria, parasites, fungi), including antimicrobial resistance (AMR). This article outlines WastPan's experiences in this project, criteria target selection, sampling locations, frequency, analysis methods results communication. Target selection relied on...

10.1016/j.scitotenv.2024.171401 article EN cc-by-nc The Science of The Total Environment 2024-03-11

Abstract Background Antimicrobial resistance (AMR) is a critical threat to human health. Escherichia coli and Klebsiella pneumoniae are clinically the most important species associated with AMR common carbapenemase-producing (CP) Enterobacterales detected in specimens Finland. Wastewater surveillance has emerged as potential approach for population-level of AMR, wastewater could offer reflection from larger population one sample minimal recognized ethical issues. In this study, we...

10.1186/s13756-024-01370-z article EN cc-by Antimicrobial Resistance and Infection Control 2024-01-30

The fungal genus Heterobasidion includes some of the most devastating conifer pathogens in boreal forest region. In this study, we showed that alphapartitivirus partitivirus 13 from annosum (HetPV13-an1) is main causal agent severe phenotypic debilitation host fungus. Based on RNA sequencing using isogenic virus-infected and cured strains, HetPV13-an1 affected transcription 683 genes, which 60% were downregulated 40% upregulated. Alterations observed carbohydrate amino acid metabolism...

10.1128/jvi.01744-17 article EN Journal of Virology 2017-12-13

Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants may have different characteristics, e.g., in transmission, mortality, and the effectiveness of vaccines, indicating importance variant detection at population level. Wastewater-based surveillance SARS-CoV-2 RNA fragments has been shown to be an effective way monitor COVID-19 pandemic Wastewater is a complex sample matrix affected by environmental factors PCR inhibitors, causing insufficient coverage sequencing,...

10.1038/s41598-024-58113-8 article EN cc-by Scientific Reports 2024-04-02

Abstract Around the world, influenza A virus has caused severe pandemics, and risk of future pandemics remains high. Currently, surveillance is based on clinical diagnosis reporting disease cases. In this study, we apply wastewater-based to monitor incidence at population level. We report data in 10 wastewater treatment plant catchment areas covering 40% Finnish population. Altogether, 141 monthly composite influent samples (collected between April 2020 May 2021) were analysed from...

10.1101/2023.08.28.23294723 preprint EN medRxiv (Cold Spring Harbor Laboratory) 2023-08-28

The purpose of this study was to evaluate the potential wastewater-based surveillance in monitoring epidemics at national level Finland. 2021–2022 respiratory syncytial virus epidemic Finland analyzed from wastewater and Finnish National Infectious Diseases Register. performed using 150 samples that were collected monthly May 2021 July 2022 ten treatment plants cover 40% population. Respiratory detection 24 h composite influent reverse-transcription quantitative polymerase chain reaction...

10.1021/acsestwater.3c00752 article EN cc-by-nc-nd ACS ES&T Water 2024-05-09

Background Many countries have applied the wastewater surveillance of COVID-19 pandemic to their national public health monitoring measures. The most used methods for detecting SARS-CoV-2 in are quantitative reverse transcriptase–polymerase chain reaction (RT-qPCR) and transcriptase–droplet digital polymerase (RT-ddPCR). Previous comparison studies produced conflicting results, thus more research on subject is required. Objective This study aims compare RT-qPCR RT-ddPCR wastewater. It also...

10.2196/53175 article EN cc-by JMIR Public Health and Surveillance 2024-08-19

Abstract The spatial and temporal changes of the COVID-19 pandemic have been monitored with wastewater-based surveillance, which many countries applied to their national public health monitoring measures. most commonly used methods for detection SARS-CoV-2 in wastewater are RT-qPCR RT-ddPCR. Previous comparisons two produced conflicting results; some found RT-ddPCR be more sensitive, one others them equal sensitivity. This research was conducted further study these as well different RNA...

10.1101/2023.09.07.23295183 preprint EN medRxiv (Cold Spring Harbor Laboratory) 2023-09-08

The fungal genus Heterobasidion includes forest pathogenic species hosting a diverse group of partitiviruses. They include the host debilitating partitivirus 13 strain an1 (HetPV13-an1), which was originally observed in slowly growing H. annosum 94233. In this study, relatively fast-growing sector 94233-RC3 isolated from highly debilitated mycelial culture 94233, and its gene expression virus transcript quantities as well genomic sequence HetPV13-an1 were examined. genome identical to that...

10.3389/fmicb.2021.661554 article EN cc-by Frontiers in Microbiology 2021-10-14

Abstract The purpose of this study was to evaluate the potential wastewater-based surveillance in monitoring epidemics at national level Finland. To discover correlation wastewater data and register data, 2021–2022 respiratory syncytial virus (RSV) epidemic Finland analyzed from Finnish National Infectious Diseases Register. performed using samples that were collected monthly May 2021 July 2022 ten treatment plants cover 40% population. Respiratory detection 24-h composite influent RT-qPCR....

10.1101/2023.09.04.23295011 preprint EN medRxiv (Cold Spring Harbor Laboratory) 2023-09-05

<sec> <title>BACKGROUND</title> Many countries have applied the wastewater surveillance of COVID-19 pandemic to their national public health monitoring measures. The most used methods for detecting SARS-CoV-2 in are quantitative reverse transcriptase–polymerase chain reaction (RT-qPCR) and transcriptase–droplet digital polymerase (RT-ddPCR). Previous comparison studies produced conflicting results, thus more research on subject is required. </sec> <title>OBJECTIVE</title> This study aims...

10.2196/preprints.53175 preprint EN 2023-09-28
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