A5016985163- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Viral gastroenteritis research and epidemiology
- Meat and Animal Product Quality
- Food Safety and Hygiene
- Phytochemicals and Antioxidant Activities
- Veterinary medicine and infectious diseases
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Phytochemical and Pharmacological Studies
- Antimicrobial Resistance in Staphylococcus
- Spectroscopy and Chemometric Analyses
- Essential Oils and Antimicrobial Activity
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
- Food Quality and Safety Studies
- Trace Elements in Health
- Nutritional Studies and Diet
- Animal Disease Management and Epidemiology
- Bacterial Infections and Vaccines
- Bacterial biofilms and quorum sensing
- Microbial Inactivation Methods
- Advanced Chemical Sensor Technologies
- Botanical Research and Applications
Estonian University of Life Sciences
2014-2024
Veterinary and Food Board
2012-2019
Abstract In response to the EFSA call New approaches in identifying and characterizing microbial chemical hazards, project INNUENDO (https://sites.google.com/site/theinnuendoproject/) aimed design an analytical platform standard procedures for use of whole-genome sequencing surveillance outbreak investigation food-borne pathogens. The firstly attempted identify existing flaws needs, then provide applicable cross-sectorial solutions. focused developing a small countries with limited...
Since 2005 campylobacteriosis has been the most commonly reported gastrointestinal infection in humans European Union with more than 200,000 cases annually. Also Campylobacter is one of frequent cause food-borne outbreaks 319 to EFSA, involving 1,254 disease and 125 hospitalizations EU 2019. Importantly poultry meat common source for sporadic infections strong-evidence EU. In present study, 429 fresh broiler chicken samples Estonian, Latvian, Lithuanian origin were collected from Estonian...
The decreased costs of genome sequencing have increased the capability to apply whole-genome epidemiological surveillance zoonotic
Genotypes of Campylobacter jejuni in Baltic fresh broiler chicken meat and Estonian human origin samples were determined by using MLST. We observed associations between clonal complexes, sequence types, antibiotic resistance C. isolates. The detection high proportions fluoroquinolone among Latvian (100.0%) Lithuanian (84.6%) isolates, found this study, suggests the wide use these antibiotics poultry countries. MLST 48 isolates revealed a total 22 types belonging to 13 complexes. From...
Summary The resistance patterns of Campylobacter spp. isolated from retail broiler chicken meat originating either Estonia, Lithuania or Latvia collected in Estonia were determined. Additionally, collaboration with the laboratories several Estonian hospitals, antimicrobial susceptibility determined for isolates patients severe enteric infections. identified at species level by PCR method. Respectively, 88.8% C. jejuni, and 11.2% coli . In total, 126 human origin tested minimal inhibitory...
The reservoir and source of human campylobacteriosis is primarily considered to be poultry, but also other such as ruminants, pets environmental sources are related with infection burden. Multilocus sequence typing often used for Campylobacter epidemiological studies determine potential infections. collection 420 jejuni isolates assigned MLST genotype from poultry (n = 139), cattle 48) wild birds 101) were in attribution analysis. Asymmetric island model accurate congruent self-attribution...
Poultry meat is considered the most important source of Campylobacter spp. Because rising antimicrobial resistance in spp., this study investigated isolates from fresh broiler chicken originating Baltic countries sold Estonian retail settings. Additionally, human clinical obtained patients with enteritis Estonia were analysed. The aim was to investigate susceptibility nalidixic acid, ciprofloxacin, tetracycline, streptomycin, erythromycin and gentamicin. broth microdilution method EUCAMP2...
Plant pomaces in suitable forms (powders, extracts) can be used foods of animal origin to increase the nutritional value and safety these foods. In present study, water extracts apple, black currant, rhubarb tomato were fish marinade solutions evaluate their effect on growth dynamics microorganisms potential Listeria monocytogenes by challenge testing. The results showed that mesophilic aerobic microorganisms, Pseudomonas spp., yeasts moulds remained at acceptable levels throughout...
Listeria monocytogenes sequence type 1247 clonal complex 8 caused a prolonged multicountry outbreak in five EU countries: Denmark, Estonia, Finland, France, and Sweden. A total of 22 disease cases were identified with onset symptoms between July 2014 February 2019. Five patients died due to, or with, the disease. The retrospective analysis L. isolate VLTRLM2013 revealed presence an outbreak-related strain (cgMLST L2-SL8-ST1247-CT4158) ready-to-eat fish product more than year prior to first...
Increasing antimicrobial resistance (AMR) challenges conventional antibiotics, prompting the search for alternatives. Extracellular vesicles (EVs) from pasteurised cattle milk offer promise, due to their unique properties. This study investigates efficacy against five pathogenic bacteria, including Staphylococcus aureus ATCC 25923, aiming combat AMR and develop new therapies. EVs were characterised tested using various methods. Co-culture experiments with S. showed significant growth...
Consumer interest in healthier meat products has grown recent years. Therefore, the use of plant powders as natural preservatives composition pork and beef could be an alternative to traditional products. This study aimed assess effect different powders, such blackcurrant, chokeberry, rowan berries, apple, tomato, garlic, rhubarb, on microbial growth dynamics minced during refrigerated storage. The total counts aerobic microorganisms, Pseudomonas spp., yeasts, molds were examined according...
Plant powders with antimicrobial properties can be used in food manufacturing and must comply the demands of consumers regarding microbiological safety, nutritional value, sensory foods. The present study aimed to assess microbial growth inhibitory ability different plant powders, including by-products horticultural primary processing (e.g., pomace) raw cooked minced pork. total counts aerobic mesophilic bacteria, pseudomonads, yeasts, moulds were studied dynamics meat samples. Additionally,...
Pigs are known as the main Campylobacter coli reservoirs. Campylobacteriosis, most commonly reported gastrointestinal disease in humans, is mainly caused by consumption of poultry meat, and little about role pork. often associated with C. coli, including antimicrobial-resistant isolates. Therefore, entire pork production chain must be considered an important source coli. This study aimed to determine antimicrobial resistance spp. isolated from caecal samples fattening pigs at Estonian...
Background: Salmonella enterica represents a considerable public concern worldwide, with farm animals often recognised as an important reservoir. This study gives overview of the prevalence and serotype diversity over 5-year period in meat production chain Estonia. Data on human salmonellosis same are provided. Methods: surveillance data from 2016 to 2020 were analysed. Results: The at level was 27.7%, 3.3% 0.1% for fattening pigs, cattle poultry, respectively. S. Derby most prevalent pigs...
Shiga toxin-producing Escherichia coli (STEC) O157:H7 was isolated from 30 (4%) of 744 cattle hide swab samples collected at Estonian slaughterhouses within a 3-year monitoring program zoonotic pathogens. The isolates were characterized by determining the presence STEC main virulence factors, antimicrobial resistance profiles, and genetic relatedness pulsed-field gel electrophoresis (PFGE). Thirteen strains carried stx2 gene alone 17 both stx1 genes. most frequently detected stx subtype...
We sequenced the genome of a multicountry outbreak-related Listeria monocytogenes sequence type 1247 strain (VLTRLM2013) that was isolated from vacuum-packaged sliced salted salmon product an Estonian fish-processing company obtained retail outlet in 2013.
Abstract The decreased costs of genome sequencing have increased capability to apply whole-genome sequence on epidemiological surveillance zoonotic Campylobacter jejuni . However, knowledge about how genetically similar epidemiologically linked isolates can be is vital for correct application this methodology. To address issue in C. we investigated the spatial and temporal signals genomes a major clonal complex generalist lineage, ST-45 CC, by exploiting population structure genealogy...
Broiler chicken meat is considered as the main source of foodborne Campylobacter infection. jejuni multilocus sequence type ST-5 has been previously identified predominant among Latvian and Lithuanian broiler products often isolated from patients with enteric The market share origin fresh sold at Estonian retail estimated close to 50%. In this study, we used whole-genome sequences C. isolates obtained retail. Applied genome-by-genome MLST revealed high similarity investigated 1589 shared...