A5007355528- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Listeria monocytogenes in Food Safety
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Botulinum Toxin and Related Neurological Disorders
- Hereditary Neurological Disorders
- Microbial Inactivation Methods
- Enterobacteriaceae and Cronobacter Research
- Antibiotic Resistance in Bacteria
- Essential Oils and Antimicrobial Activity
- Fungal and yeast genetics research
- Consumer Attitudes and Food Labeling
- Potato Plant Research
- Enzyme-mediated dye degradation
- Phytoestrogen effects and research
- Neurological disorders and treatments
- Clostridium difficile and Clostridium perfringens research
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Food Security and Health in Diverse Populations
- Bacterial biofilms and quorum sensing
- Child Nutrition and Water Access
- Plant-based Medicinal Research
- Trace Elements in Health
Istituto Superiore di Sanità
2004-2024
European Union
2015
Sapienza University of Rome
2001
Shiga toxin (Stx)-producing Escherichia coli (STEC) are pathogenic E. causing diarrhea, hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). STEC characterized by a constellation of virulence factors additional to Stx have long been regarded as capable cause HC HUS when possessing the ability inducing attaching effacing (A/E) lesion enterocyte, although strains isolated from such severe infections sometimes lack this feature. Interestingly, capability A/E is shared with another...
Plasmids that encode certain subtypes of the botulinum neurotoxin type B have recently been detected in some Clostridium strains. The objective present study was to investigate frequency with which plasmid carriage gene (bont/B) occurs strains C. B, Ab, and A(B), whether is bont/B subtype-related.PCR-Restriction fragment length polymorphism employed identify gene. Pulsed-field gel electrophoresis Southern blot hybridization specific probes were performed analyze genomic location subtype...
SUMMARY We describe a foodborne outbreak in Italy caused by enteroinvasive Escherichia coli (EIEC), an enteric pathogen uncommon industrialized countries. On 14 April 2012 number of employees the city Milan Fire Brigade (FB) were admitted to hospital with severe diarrhoea after attending their canteen. Thirty-two patients hospitalized and total 109 cases identified. A case-control study conducted on 83 32 controls canteen without having symptoms identified cooked vegetables be significantly...
In summer 2013, an excess of paediatric cases haemolytic uraemic syndrome (HUS) in a southern region Italy prompted the investigation community-wide outbreak Shiga toxin 2-producing Escherichia coli (STEC) O26:H11 infections. Case finding was based on testing patients with HUS or bloody diarrhoea for STEC infection by microbiological and serological methods. A case–control study conducted to identify source outbreak. O26 identified 20 children (median age 17 months) HUS, two whom reported...
Internalin A (InlA), a cell wall-bound protein of Listeria monocytogenes, is among the major components involved in adhesion to and invasion host cells expressing specific forms E-cadherin. Some L. monocytogenes strains secrete truncated non-functional InlA. The purpose this study compare biofilm-forming abilities from clinical sources InlA proteins different forms. total 70 were examined using SDS-PAGE, Western blot, DNA sequencing, microtitre plate biofilm formation assays. We found that 8...
We describe a strategy to identify the clusters of genes encoding components botulinum toxin type A (boNT/A) complexes in 57 strains Clostridium types A, Ab, and A(B) isolated Italy United States from different sources. Specifically, we combined results PCR for detecting ha33 and/or p47 with those boNT/A PCR-restriction fragment length polymorphism analysis. Three gene were revealed; A1 was predominant among States, whereas A2 predominated Italian strains, suggesting geographic distinction...
Purpose. Shiga toxin-producing Escherichia coli (STEC) represents a major issue for public health because of the severity associated illnesses, including haemolytic uraemic syndrome (HUS). In 2015, investigation case HUS revealed an outbreak toxin-2-producing E. O26 : H11 infection in nursery Italy. The showed that was transmitted to cases' contacts via person person.Methods. finding performed by testing STEC stool samples case's within family and nursery. isolates were characterized whole...
The partial nucleotide sequence ( approximately 10 kb) of the cluster genes encoding botulinum neurotoxin complex in Clostridium type A strain Mascarpone was determined. analysis revealed six ORFs (orfs), which were organized as A2 and A3 gene clusters strains Kyoto-F NCTC 2916, respectively. While orfs at proximal distal ends (orfX2 bont/A genes) shared a high level similarity with corresponding sequences Kyoto-F, segment encompassing orfX1 botR/A within exhibited higher degree homology to...
Enteroaggregative Haemorrhagic E. coli (EAHEC) is a new pathogenic group of characterized by the presence vtx2-phage integrated in genomic backbone (EAggEC). So far, four distinct EAHEC serotypes have been described that caused, beside large outbreak infection occurred Germany 2011, small and six sporadic cases HUS time span 1992–2012. In present work we determined whole genome sequence vtx2-phage, termed Phi-191, first O111:H2 isolated France 1992 compared it with those vtx-phages whose...
We analyzed 27 Listeria monocytogenes strains of serotypes 1/2b and 4b, from invasive gastroenteric listeriosis, for molecular experimental virulence. Molecular virulence was tested by PCR the presence 8 major virulence-associated genes genetic polymorphisms through restriction enzyme analysis; genomic DNA typing using pulsed-field gel electrophoresis also performed. Experimental evaluated intra-peritoneal intra-gastric mouse assays. Our results showed no significant differences in...
Shiga toxin-producing Escherichia coli (STEC) O157:H7 was isolated from 30 (4%) of 744 cattle hide swab samples collected at Estonian slaughterhouses within a 3-year monitoring program zoonotic pathogens. The isolates were characterized by determining the presence STEC main virulence factors, antimicrobial resistance profiles, and genetic relatedness pulsed-field gel electrophoresis (PFGE). Thirteen strains carried stx2 gene alone 17 both stx1 genes. most frequently detected stx subtype...