A5027529308- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Fungal Biology and Applications
- Traditional and Medicinal Uses of Annonaceae
- Plant Pathogens and Fungal Diseases
- Microbial Metabolism and Applications
- Natural product bioactivities and synthesis
- Alkaloids: synthesis and pharmacology
- Chemical Synthesis and Analysis
- Genomics and Phylogenetic Studies
- Phytochemistry and Bioactive Compounds
- Asymmetric Synthesis and Catalysis
- Phytochemistry and Biological Activities
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Protein Degradation and Inhibitors
- Analytical Chemistry and Chromatography
- Essential Oils and Antimicrobial Activity
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Environmental Quality and Pollution
- Inorganic and Organometallic Chemistry
- Phytochemical compounds biological activities
- Single-cell and spatial transcriptomics
- Immune cells in cancer
Zhejiang University
2020-2025
Zhejiang Ocean University
2020-2025
Second Affiliated Hospital of Zhejiang University
2023-2024
Sir Run Run Shaw Hospital
2024
Artificial Intelligence in Medicine (Canada)
2024
Huashan Hospital
2024
Institute of Dermatology
2024
Fudan University
2024
Advanced Neural Dynamics (United States)
2019
Zhejiang Gongshang University
2017-2018
ABSTRACT Chemical investigation of the marine‐derived fungus Trichoderma harzianum ZN‐4 led to isolation and identification seven new secondary metabolites, harzianolides K–Q ( 1 – 7 ). The complete structures compounds were determined by high‐resolution mass spectrometry nuclear magnetic resonance spectroscopic analyses coupled with electronic circular dichroism calculations. isolates inactive as antibacterial antitumor agents. However, K ), L 2 M 3 ) O 5 exhibited weak moderate anti‐...
Two unusual alkaloids with furanopyrrolidine skeleton, harzianopyrrolidones A-B (1-2), four new compounds, harzianopyridone B (3), harzianopyrone (4), (R)-1-(3,5-dihydroxy-4-methylphenyl)-1-hydroxyhept-5E-en-2-one (5), and harzianopyrrolidone C (6), together two known compounds (7-8) were isolated from a marine-derived fungus Trichoderma harzianum ZN-4. Structures of the elucidated based on their HRESIMS data, extensive NMR spectroscopic analyses, calculation in combination DP4+, ECD...
Five new unusual citrinin-derived alkaloids with a tetracyclic core, citrinidines A-E (1-5), two amide alkaloids, methyl (2S,8E)-1'-(2-methyl-3-oxodec-8-enamido) butanoate (6) and (2S,8E)-2-methyl-3-oxodec-8-enamide (7), citrinin trimer, tricitrinol C (8), acetal-ketal derivative, citrininol (9), together four known monomers (10-13), three dimers (14-16), were isolated from the fermentation of hydrothermal vent-associated fungus Penicillium citrinum TW132-59. Their structures unambiguously...
Natural product discovery is hindered by the lack of tools that integrate untargeted nuclear magnetic resonance and mass spectrometry data on a library scale. This article describes first application innovative NMR/MS-based machine learning tool, "Structure-Oriented Fractions Screening Platform (SFSP)", enabling functional-group-guided fractionation accelerating characterization undescribed natural products. The concept was applied to extract marine fungus known be prolific producer diverse...
Abstract Peptide–drug conjugates (PDCs) have emerged as a promising class of targeted therapeutics with substantial pharmaceutical advantages and market potentials, which is combination peptide (selective to the disease-relevant target), linker (stable in circulation but cleavable at target site) cytotoxic/radioactive drug (efficacious/traceable for disease). Among existing PDCs, those based on radiopharmaceuticals (a.k.a. radioactive drugs) are valued due their accurate imaging destruction...
We report the discovery of talaropeptins A (1) and B (2), tripeptides with an unusual 5/6/5 heterocyclic scaffold N-trans-cinnamoyl moiety, which were identified from marine-derived fungus Talaromyces purpureogenus CX11. bioinformatic analysis genome T. CX11 gene inactivation revealed that biosynthesis involves a nonribosomal peptide synthase cluster. Their chemical structures elucidated using combination 1D 2D NMR spectroscopy mass spectrometry. The absolute configurations 1 2 established...
Abstract Large-scale studies of single-cell sequencing and biological experiments have successfully revealed expression patterns that distinguish different cell types in tissues, emphasizing the importance studying cellular heterogeneity accurately annotating types. Analysis gene profiles these provides two essential data for type annotation: annotated references canonical markers. In this study, first comprehensive database transcriptomic annotation resource (CellSTAR) was thus developed....
Introduction: One new levantilide D (2) with a 20-membered macrolide, along one known C (1), was isolated from rare marine actinomycetes Micromonospora sp. (FIMYZ52). Method: The structural elucidation of the compounds based on integrated analyses involving 1D and 2D NMR spectroscopy HR-ESI-MS. Result: relative absolute configurations 1 2 were ascertained by integrating multispectral ROESY NOESY information analogs in conjunction theoretical TDDFT-ECD calculations. Levantilide exhibited...
In nature, secondary metabolites have been proven to be the essential communication media between co-occurring microorganisms and influence their relationship with each other. this study, we conducted a metabolomics survey of an artificial co-culture related hydrothermal vent fungal-bacterial community comprising Aspergillus sclerotiorum Streptomyces reciprocal relationship. The fungal strain was found increase secretion notoamides compound cyclo(Pro-Trp) produced by actinomycetes discovered...
In this study, chemical profiles for two co-existing deep-sea-derived Penicillium fungal strains were thoroughly investigated. Two new compounds and 11 known identified from sp. LXY140-R, while one compound 12 isolated LXY140-3. Their structures elucidated by extensive 1D 2D NMR experiments, which supported HR-ESI-MS data. The antiproliferative activities of all isolates against HCT-116, A549 Bel-7402 cell lines also evaluated. Compounds 2, 5, 6, 10 13 showed potent activity. To reveal the...
Chemical investigation of the aerial part Illigera aromatic S.Z.Huang & S.L. Mo has resulted in isolation and characterization one new aporphine alkaloid, illigerine B (1), along with three known analogues laurodionine (2), N-formyl-laurolitsine (3) A (4).Their structures were established by spectrometric means physico-chemical properties.The vitro cytotoxic activities compound 1 against Hela, SMMC7721, Bcap37 cell lines evaluated.Compound exhibited moderate activity tumor types, IC50 values...
The measurement of cell-based molecular bioactivity (CMB) is critical for almost every step drug development. With the booming application AI in biomedicine, it essential to have CMB data promote learning patterns guiding modern discovery, but no database providing such information has been constructed yet. In this study, we introduce MolBiC, a knowledge base designed describe valuable on measured within cellular context. MolBiC features 550 093 experimentally validated CMBs, encompassing...
Background: Marine fungi have been recognized as new sources for the discovery of valuable secondary metabolites, providing lead compounds drug and development. Objective: The objective this study was to isolate identify metabolites from marine-derived fungus Trichoderma harzianum ZN-4 evaluate their antifungal activity. Method: Fungus strain initially fermentated using a PDB liquid medium. Subsequently, extensive chromatographic methods were applied fermentation cultures ZN-4, chemical...
Marine actinomycetes are known for their production of remarkable organic molecules, particularly those featuring polyoxygenated long-chain backbones. Determining the absolute configurations these compounds remains a challenging task even today. In this study, we successfully established planar structures and two highly flexible amide alkaloids from Streptomyces sp. WU20: kueishanamides A (1) B (2). These possess C13 linear backbone each contains five stereogenic carbon centers. Our approach...